Probe-based confocal laser endomicroscopy of the airways: physiological and pathological characteristics of preneoplastic and neoplastic lesions

Trindade, J.; Figueiredo, Vivíane Rossi; Pêgo-Fernandes, Paulo Manuel; Terra, Ricardo Mingarini

doi:10.1590/1806-3713/e20180103

Cited by 2 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, animals can die during the surgery and the optical window can break or detach after successful implantation. These problems can be overcome by the use of optical fibres that provide access to sites deeper within the organ [ 141 , 142 ], or with micro-endoscopic probes employed to provide a less invasive observation mode of cellular-level features in colon [ 143 , 144 ], oesophagus [ 145 , 146 ], and trachea [ 147 ]. Fluorescent micro-endoscopy is a powerful technique for imaging the internal structure of a hollow organ; however, it can have a lower resolution and smaller field of view compared to high-resolution microscopes used in the techniques above.…”

Section: Biological Models For Fluorescence Imaging; From the Monolayer Culture To The Whole Organmentioning

confidence: 99%

Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations

Hickey

Ung

Bader

et al. 2021

Cells

View full text Add to dashboard Cite

Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell biology, but the complexities of fluorescence microscopy as an imaging tool can sometimes be overlooked or misunderstood. This review seeks to cover the three fundamental considerations when designing fluorescence microscopy experiments: (1) hardware availability; (2) amenability of biological models to fluorescence microscopy; and (3) suitability of imaging agents for intended applications. This review will help equip the reader to make judicious decisions when designing fluorescence microscopy experiments that deliver high-resolution and informative images for cell biology.

show abstract