Genetic and symbiotic characterization of rhizobia nodulating legumes in a mining area in southeast Brazil

Lopez, Bruna Daniela Ortiz; Teixeira, Anita Fernanda dos Santos; Michel, Daniele Cabral; Guimarães, Amanda Azarias; Costa, Amanda Monique da; Costa, Juliana Santos; Pereira, Marcela de Souza; Duarte, Bárbara Luyse Marques; Moreira, Fátima Maria de Souza

doi:10.1590/1678-992x-2020-0238

Cited by 4 publications

(1 citation statement)

References 43 publications

(35 reference statements)

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“…For Basic Protocol 3, the beginning of symbiosis may be affected by the size of the seeds, because larger seeds have greater energy reserves and a longer time to cotyledon senescence, which can improve symbiosis (López et al., 2022). To ensure symbiosis, it is important to perform a second inoculation when the tap and secondary roots are already developed.…”

Section: Commentarymentioning

confidence: 99%

Biological Efficacy of Plant Growth–Promoting Bacteria and Arbuscular Mycorrhizae Fungi: Assessments in Laboratory and Greenhouse Conditions

2023

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Utilizing the interactions of microorganisms with plants offers a favorable path to increase crop production and replace the use of synthetic fertilizers. Different bacteria and fungi have been used as biofertilizers to improve agricultural production, yield, and sustainability. Beneficial microorganisms can act as free-living organisms, symbiotes, and endophytes. Soil bacteria called plant growth-promoting bacteria (PGPB) and fungi called arbuscular mycorrhizae fungi (AMF) stimulate the growth and health of plants by direct and indirect mechanisms including nitrogen fixation, phosphorus solubilization, phytohormone production, enzyme production, antibiotic synthesis, and induced systemic resistance. To use these microorganisms as a biofertilizer, it is necessary to assess their efficacy under laboratory and greenhouse conditions. Few reports detail the methods used to develop a test under different environmental conditions, and without these details it is difficult to develop suitable methodologies to evaluate microorganism-plant relationships. We describe four protocols that go from sample preparation to testing in vitro the efficacy of different biofertilizers. Each protocol can be used to test a different biofertilizer microorganism, focusing on bacteria such as Rhizobium sp., Azotobacter sp., Azospirillum sp., Bacillus sp. as well as AMF such as Glomus sp. These protocols can be used in several stages of biofertilizer development, including microorganism selection, microorganism characterization, and in vitro evaluation of efficacy for the registration process.

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Section: Commentarymentioning

confidence: 99%

Biological Efficacy of Plant Growth–Promoting Bacteria and Arbuscular Mycorrhizae Fungi: Assessments in Laboratory and Greenhouse Conditions

2023

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Nodule Formation and Nitrogen Fixation in Acacia holosericea Plants Grown in Soil Admixed with Iron Ore Tailings

You

et al. 2022

J Soil Sci Plant Nutr

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Genetic identification of potential nodulating bacteria and nodule-associated bacteria (NAB) within root nodules of Inga punctata trees in a Costa Rican Cloud Forest

Eaton,

Hamilton,

Chen

et al. 2023

Preprint

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Background and Aims Leguminous tree root nodules have N-fixing, nodulating bacteria important for soil C and N cycle recovery following forest disturbance, and nodule-associated bacteria (NAB), which often provide plant growth promoting (PGP) activities. However, composition and function of these microbiomes within tropical leguminous forest trees is understudied. Methods Root nodules were collected from 1, 2, 13-year-old and old growth Inga punctata trees within a tropical cloud forest. Nodule DNA was extracted, from which bacterial taxa were identified through Illumina DNA sequencing methods. Results Bradyrhizobium diazoefficiensDNA sequences represented 63% to 86% and NAB sequences represented 7% to 12% of the total bacterial taxa in the nodules. Collectively, 26 of the NAB taxa were identified with 9 PGP activities. The 1 year-old nodules had the greatest percentages of PGP NAB taxa, which decreased with tree age, though the percentage of total NAB DNA increased with tree age, as did the NAB community complexity. Conclusions Bradyrhizobium diazoefficiensappears to be the root nodule-forming bacteria, while the NAB genera with known PGP activities appear to provide important functions that benefit both the nodule microbiome and I. punctata. The greater percentage of PGP NAB in the youngest nodules suggests their importance in early growth and development of I. punctata and its root nodules, the great presence of NAB DNA in all nodules suggests the importance of NAB throughout the lifespan of I. punctata, and the increasing NAB community complexity with tree age suggests the microbiome undergoes parallel succession with the tree.

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Genetic and symbiotic characterization of rhizobia nodulating legumes in a mining area in southeast Brazil

Cited by 4 publications

References 43 publications

Biological Efficacy of Plant Growth–Promoting Bacteria and Arbuscular Mycorrhizae Fungi: Assessments in Laboratory and Greenhouse Conditions

Biological Efficacy of Plant Growth–Promoting Bacteria and Arbuscular Mycorrhizae Fungi: Assessments in Laboratory and Greenhouse Conditions

Nodule Formation and Nitrogen Fixation in Acacia holosericea Plants Grown in Soil Admixed with Iron Ore Tailings

Genetic identification of potential nodulating bacteria and nodule-associated bacteria (NAB) within root nodules of Inga punctata trees in a Costa Rican Cloud Forest

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