Detection of differentially methylated regions of irradiated fig tree selections

Rodrigues, Maria Gabriela Fontanetti; Soares, Murilo Racy; Gimenez, Daniele Fernanda Jovino; Fonseca, Larissa Fernanda Simielli; Torrieri, Erico; Ramos, Ester Silveira; Giuliatti, Silvana

doi:10.1590/1678-992x-2016-0195

Cited by 2 publications

(1 citation statement)

References 70 publications

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“…Among these, DNA methylation is important and affects gene expression in plants and animals [ 77 ]. DNA methylation occurs at cytosine base, within CpG dinucleotide or may occur at CpNpG (N = A, C or T) sites [ 78 ]. CpG rich regions are named as CpG islands and to classify a genome region as CpG island, three conditions must be fulfilled (i) GC content should be above 50% (ii) length of CpG/CpNpG region should be greater than 200 bp (iii) ratio of observed-to-expected CpG dinucleotide number should be above 0.6 [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

In-silico analysis of cis-acting regulatory elements of pathogenesis-related proteins of Arabidopsis thaliana and Oryza sativa

et al. 2017

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Pathogenesis related (PR) proteins are low molecular weight family of proteins induced in plants under various biotic and abiotic stresses. They play an important role in plant-defense mechanism. PRs have wide range of functions, acting as hydrolases, peroxidases, chitinases, anti-fungal, protease inhibitors etc. In the present study, an attempt has been made to analyze promoter regions of PR1, PR2, PR5, PR9, PR10 and PR12 of Arabidopsis thaliana and Oryza sativa. Analysis of cis-element distribution revealed the functional multiplicity of PRs and provides insight into the gene regulation. CpG islands are observed only in rice PRs, which indicates that monocot genome contains more GC rich motifs than dicots. Tandem repeats were also observed in 5’ UTR of PR genes. Thus, the present study provides an understanding of regulation of PR genes and their versatile roles in plants.

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Section: Resultsmentioning

confidence: 99%

In-silico analysis of cis-acting regulatory elements of pathogenesis-related proteins of Arabidopsis thaliana and Oryza sativa

et al. 2017

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Effect of Leptosphaeria maculans Infection on Promoter DNA Methylation of Defence Genes in Brassica napus

et al. 2020

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Canola (Brassica napus) is an important crop species grown worldwide for its seeds, which are crushed for edible oil. Canola production is threatened by blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, which can lead up to 100% yield loss. A plant’s disease resistance response depends highly on the regulation of the expression of defence genes. DNA methylation, an epigenetic mark, is one of the most important regulatory mechanisms in a plant’s defence system. Here, the DNA methylation pattern of promoters of defence genes has been investigated between leaves from control and infected plants with L. maculans of resistant and susceptible cultivars of B. napus. In total, over 12,000 promoters were found to be differentially methylated between leaves from control and infected plants in the two cultivars, of which 225 promoters were related to defence genes and differentially methylated between the resistant and susceptible cultivars. The majority of defence gene promoters were hypo- or hyper-methylated in the first and second true leaves, but not in the third and fourth leaves. The outcomes will assist in developing an insight into genome-wide DNA methylation patterns in canola cultivars, and ultimately help breeders to optimise the breeding programmes for enhancing resistance against blackleg disease.

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Detection of differentially methylated regions of irradiated fig tree selections

Cited by 2 publications

References 70 publications

In-silico analysis of cis-acting regulatory elements of pathogenesis-related proteins of Arabidopsis thaliana and Oryza sativa

In-silico analysis of cis-acting regulatory elements of pathogenesis-related proteins of Arabidopsis thaliana and Oryza sativa

Effect of Leptosphaeria maculans Infection on Promoter DNA Methylation of Defence Genes in Brassica napus

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