Antileishmanial activity and immunomodulatory effect of secosubamolide, a butanolide isolated from Nectandra oppositifolia (Lauraceae)

Costa-Silva, Thaís A.; Conserva, Geanne A. Alves; Galisteo, Andrés Jimenez; Tempone, André G.; Lago, João Henrique G.

doi:10.1590/1678-9199-jvatitd-2019-0008

Cited by 8 publications

(5 citation statements)

References 36 publications

(33 reference statements)

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“…As observed in the study by Costa-Silva et al. ( 36 ), isolates from natural products, despite presenting an SI less than 10, had immunomodulatory activity against L. infantum , reducing the production of Th2 profile cytokines, in addition to stimulating chemokines associated with a decrease in the parasite load.…”

Section: Discussionmentioning

confidence: 61%

“…Although the extracts from both A. platensis and D. tertiolecta studied here do not present an SI greater than 10 in assays with human cells, we do not exclude the immunomodulatory potential of these extracts. As observed in the study by Costa-Silva et al (36), isolates from natural products, despite presenting an SI less than 10, had immunomodulatory activity against L. infantum, reducing the production of Th2 profile cytokines, in addition to stimulating chemokines associated with a decrease in the parasite load.…”

Section: Discussionmentioning

confidence: 64%

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Therapeutic Potential of Photosynthetic Microorganisms for Visceral Leishmaniasis: An Immunological Analysis

et al. 2022

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New therapeutic strategies for visceral leishmaniasis (VL) have been studied, and the development of an immunotherapeutic agent that modulates the host’s immune response is necessary. The aim of this study was to evaluate in vitro the bioactive extracts of photosynthetic microorganisms (PMs) for their leishmanicidal/leishmanistatic and immunomodulatory potentials. Bioactive extracts from PMs (Arthrospira platensis and Dunaliella tertiolecta) were obtained by sonication. Reference drugs, miltefosine (MTF) and N-methylglucamine antimoniate (SbV), were also evaluated. The selectivity index (SI) of treatments was determined by assays of inhibitory concentration (IC50) in Leishmania infantum cells and cytotoxic concentrations (CC50) in human peripheral blood mononuclear cells by the MTT method. The immune response was evaluated in healthy human cells by the production of cytokines and nitric oxide (NO) and the gene expression of Tbx21, GATA3, RORc, and FOXP3, using four concentrations (CC50, ½ CC50, ¼ CC50, and IC50) for in-vitro stimulation. Based on the data obtained, we observed that the extracts of D. tertiolecta (SI = 4.7) and A. platensis (SI = 3.8) presented better results when compared to SbV (SI = 2.1). When analyzing the immune response results, we identified that the extracts of PMs stimulated the production of cytokines of the Th1 profile more than the reference drugs. The extracts also demonstrated the ability to stimulate NO synthesis. Regarding gene expression, in all concentrations of A. platensis extracts, we found a balance between the Th1/Th2 profile, with the average expression of the Tbx21 gene more than the GATA3 in the highest concentration (CC50). Regarding the extract of D. tertiolecta, we can observe that, in the lowest concentrations, a balance between all the genes was present, with the average expression of the GATA3 gene being lower than the others. The best result was found in the ½ CC50 concentration, stimulating a balanced positive expression between the Th1×Th17×Treg profiles, with a negative expression of GATA3. Thus, PM extracts showed promising results, presenting low toxicity, leishmanicidal/leishmanistatic activity, and induction of the immune response, which could be potential therapeutic candidates for VL.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 64%

Therapeutic Potential of Photosynthetic Microorganisms for Visceral Leishmaniasis: An Immunological Analysis

et al. 2022

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“…Interestingly, licarin A and related alkenyl butenolides, previously isolated from n- hexane extracts of N . oppositifolia [ 6 – 8 ], were not detected in the studied EtOH extracts.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, three related butenolides were isolated from the n -hexane extract of N . oppositifolia twigs, which displayed anti- Trypanosoma cruzi activity [ 7 ] and immunomodulatory effect against Leishmania infantum [ 8 ]. These trypanosomatids are etiological agents of Chagas disease and leishmaniasis, respectively [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolite profile of Nectandra oppositifolia Nees & Mart. and assessment of antitrypanosomal activity of bioactive compounds through efficiency analyses

et al. 2021

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EtOH extracts from the leaves and twigs of Nectandra oppositifolia Nees & Mart. shown activity against amastigote forms of Trypanosoma cruzi. These extracts were subjected to successive liquid-liquid partitioning to afford bioactive CH2Cl2 fractions. UHPLC-TOF-HRMS/MS and molecular networking were used to obtain an overview of the phytochemical composition of these active fractions. Aiming to isolate the active compounds, both CH2Cl2 fractions were subjected to fractionation using medium pressure chromatography combined with semi-preparative HPLC-UV. Using this approach, twelve compounds (1–12) were isolated and identified by NMR and HRMS analysis. Several isolated compounds displayed activity against the amastigote forms of T. cruzi, especially ethyl protocatechuate (7) with EC50 value of 18.1 μM, similar to positive control benznidazole (18.7 μM). Considering the potential of compound 7, protocatechuic acid and its respective methyl (7a), n-propyl (7b), n-butyl (7c), n-pentyl (7d), and n-hexyl (7e) esters were tested. Regarding antitrypanosomal activity, protocatechuic acid and compound 7a were inactive, while 7b-7e exhibited EC50 values from 20.4 to 11.7 μM, without cytotoxicity to mammalian cells. These results suggest that lipophilicity and molecular complexity play an important role in the activity while efficiency analysis indicates that the natural compound 7 is a promising prototype for further modifications to obtain compounds effective against the intracellular forms of T. cruzi.

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“…It is a member of the Lauraceae family. There are around 50 genera and 2500-3000 species in the Lauraceae family [3][4][5]. The avocado fruit, also known as the alligator pear or butter pear, has only one seed, which is covered in a hard shell and accounts for 16 percent of the total weight of the fruit [6].…”

Section: Introductionmentioning

confidence: 99%

Identification of Suitable Budwood Source through Quality Assessment of Avocado (Persea americana Mill.)

Idun

Osei

Tandoh

2022

JEAI

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Avocado (Persea americana Mill.) is gaining increasing global appeal and has received massive marketing and distribution due to its significant nutritional benefits for human health. There is little or no history on how the fruit was introduced into the country. However, raising suitable planting materials continues to be a challenge. Thus, the study sought to identify suitable budwood sources through fruit quality assessments. Mature, bruised-free fruits were harvested from five different trees at New Koforidua in the Ashanti Region of Ghana. The experimental design for this study was a Completely Randomized Design (CRD) with five replicates. Twenty-five fruits were selected from each tree for data collection and fruit analysis. The parameters studied were the physical properties (fruit weight, fruit firmness, seed weight, pulp weight, peel weight, fruit length and fruit diameter) and chemical properties [chemical composition, pH, total titratable acids (TTA) and total soluble solids (TSS)]. A sensory analysis was also conducted. The statistics were subjected to the Statistix version 10 and the means were separated using the LSD at an alpha level of 0.01. Significant differences were found in the seed weight, pulp weight, fruit length and diameter respectively, at an alpha value (p≤0.01) with “tree F” showing the highest recordings (92.03±5.57) for seed weight, (267.84±569) for pulp weight, (13.90±0.32) for fruit length and (8.44±0.12) for diameter, respectively. There was a progressive reduction in the fruit weight and fruit firmness over a period of seven and five days, respectively. There was a significant difference in the TSS and TTA at an alpha value of 0.01 with treatment J and treatment H recording the highest values of (0.36±0.02) and (1.18±0.07), respectively. The study showed that fruits of treatment F possess superior qualities than the rest of the varieties and can be a suitable budwood source.

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Antileishmanial activity and immunomodulatory effect of secosubamolide, a butanolide isolated from Nectandra oppositifolia (Lauraceae)

Cited by 8 publications

References 36 publications

Therapeutic Potential of Photosynthetic Microorganisms for Visceral Leishmaniasis: An Immunological Analysis

Therapeutic Potential of Photosynthetic Microorganisms for Visceral Leishmaniasis: An Immunological Analysis

Metabolite profile of Nectandra oppositifolia Nees & Mart. and assessment of antitrypanosomal activity of bioactive compounds through efficiency analyses

Identification of Suitable Budwood Source through Quality Assessment of Avocado (Persea americana Mill.)

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