Occurrence and antimicrobial susceptibility of enteric rods and pseudomonads isolated from the dental prostheses biofilm

Abstract

Aspiration of oral bacteria leads to cardiac and respiratory infectious diseases and dentures can act as a reservoir for pathogenic microorganisms.Objective:To determine the occurrence and the in vitro antimicrobial susceptibility of enteric rods and pseudomonads from the denture biofilm of 52 subjects at the Center for Dental Specialties of Sobral/ Ceara, Brazil.Material and Methods:Denture biofilm was collected and samples plated on MacConkey agar. The isolated bacterial colonies were identified using the BBL Crystal enteric/non-fermenter system. Antibiotic bacterial susceptibility was assessed by the disc diffusion method of amoxicillin, amoxicillin/clavulanic acid, doxycycline, tetracycline, tobramycin, imipenem, cefotaxime, and ciprofloxacin. The Minimum Inhibitory Concentration (MIC) of cefotaxime, tobramycin, doxycycline, imipenem, and ciprofloxacin was determined for 40 species by E-test.Results:34 subjects (65.4%) harbored enteric rods in their prostheses. Klebsiella pneumoniae (26.5%), Escherichia coli (23.5%), and Enterobacter aerogenes (23.5%) were the most prevalent species. All organisms were susceptible to ciprofloxacin and most species were resistant to amoxicillin or amoxicillin/clavulanic acid, demonstrating variable sensitivity patterns to other antimicrobials. However, the MIC showed the emergence of strains with reduced sensitivity to ciprofloxacin (MIC90≥3 μg/ mL) and cefotaxime (MIC90≥2 μg/mL).Conclusion:The findings show high prevalence of nosocomial diseases-related bacterial species and low susceptibility to antimicrobial drugs. Therefore, these results imply caution against the indiscriminate use of broad spectrum antibiotics in dental practice.

Antimicrobial and Mechanical Properties of Orthodontic Acrylic Resin Containing Zinc Oxide and Titanium Dioxide Nanoparticles Supported on 4A Zeolite

“…In this study, we tested three bacterial strains: Escherichia coli , Klebsiella pneumonia , and Streptococcus mutans . Previous studies performed on denture bases typically made of heat-curing polymethyl methacrylate have shown that the most colonized microorganisms on acrylic bases are Klebsiella pneumonia and Escherichia coli [ 22 ]. In the population of denture users, bad breath is due to the metabolic reactions of the proliferation of Gram-negative bacteria, including the Enterobacteriaceae family [ 23 ].…”

Isolation and identification of nonoral pathogenic bacteria in the oral cavity of patients with removable dentures

“…Unusual oral cavity microorganisms have been previously isolated from dentures and were reported by other researchers. [ 33 34 35 36 37 ] Umeda et al . reported Helicobacter pylori in the oral cavity of a patient with periodontal pockets that existed even after extirpation of the bacterium from the stomach.…”

Ill Effects and Complications Associated to Removable Dentures With Improper Use and Poor Oral Hygiene: A Systematic Review

“…All organisms were susceptible to ciprofloxacin, and most species were resistant to amoxicillin and amoxicillin-clavulanate. Therefore, preventive programs for biofilm control are essential in avoiding colonization of dental prostheses and its resulting multidrug-resistant bacterial infections [ 21 ].…”