Repair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat model

Corsetti, Adriana; Bahuschewskyj, Claudia; Ponzoni, Deise; Langie, Renan Cavalheiro; Santos, Luís Alberto dos; Camassola, Melissa; Nardi, Nance Beyer; Puricelli, Edela

doi:10.1590/1678-77572016-0094

Cited by 16 publications

(11 citation statements)

References 16 publications

(20 reference statements)

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“…As for the osteodifferentiation capability of the two cell types, both promoted the formation of mineralized tissue in vitro, although the level of expression of osteocalcin was higher in the BFPSCs. This is consistent with the accumulating evidence supporting the usage of DPSCs [ 31 , 32 , 33 , 34 , 35 ] and ASCs [ 36 , 37 , 38 , 39 , 40 ] as promising tools for bone regeneration.…”

Section: Discussionsupporting

confidence: 91%

Isolation and Characterization of Buccal Fat Pad and Dental Pulp MSCs from the Same Donor

et al. 2021

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Mesenchymal stem cells (MSCs) can be harvested from different sites in the oral cavity, representing a reservoir of cells useful for regenerative purposes. As direct comparisons between at least two types of MSCs deriving from the same patient are surprisingly rare in scientific literature, we isolated and investigated the osteoinductive potential of dental pulp stem cells (DPSCs) and buccal fat pad stem cells (BFPSCs). MSCs were isolated from the third molar dental pulp and buccal fat pads of 12 patients. The number of viable cells was quantified through manual count. Proliferation and osteodifferentiation assays, flow cytometry analysis of cell phenotypes, and osteocalcin release in vitro were performed. The isolation of BFPSCs and DPSCs was successful in 7 out of 12 (58%) and 3 out of 12 (25%) of retrieved samples, respectively. The yield of cells expressing typical stem cell markers and the level of proliferation were higher in BFPSCs than in DPSCs. Both BFP-SCs and DPSCs differentiated into osteoblast-like cells and were able to release a mineralized matrix. The release of osteocalcin, albeit greater for BFPSCs, did not show any significant difference between BFPSCs and DPSCs. The yield of MSCs depends on their site of origin as well as on the protocol adopted for their isolation. Our data show that BFP is a valuable source for the derivation of MSCs that can be used for regenerative treatments.

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Section: Discussionsupporting

confidence: 91%

Isolation and Characterization of Buccal Fat Pad and Dental Pulp MSCs from the Same Donor

et al. 2021

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“…Thus, in animals of the study group, the newly formed bone trabeculae were more mature and more efficiently filled the defect area (100.0 vs 60% in control) (see Figure 8 (b) ). The present histological results are, to some extent, comparable with reports by others, although those were mostly related to acute aseptic experiments [ 13 , 16 , 17 , 24 , 26 ].…”

Section: Resultssupporting

confidence: 93%

“…The availability and relative abundance of adipose tissue MSCs, in contrast to bone marrow cells, promotes an increasing interest in their clinical application as a source of cellular material for bone regeneration [ 14 , 15 ]. In animal studies, sizable restoration of bone tissue from adipose tissue MSCs has been demonstrated [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Technology for Repairing Osteomyelitic Bone Defects Using Autologous Mesenchymal Stromal Cells on a Collagen Matrix in Experiment

Mitrofanov¹,

Живцов²,

Orlinskaya³

et al. 2021

Sovrem Tehnol Med

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The aim of the study was to develop a technology for repairing an osteomyelitic bone defect using autologous adipose tissue mesenchymal stromal cells (MSCs) bound to a collagen matrix and to test the efficacy of this technique. Materials and Methods. The study was carried out with 17 rabbits. A bone defect was created using a milling cutter applied to the proximal third of the leg. The wound (8.0×4.0 mm and a depth of 4.0 mm) involved the periosteum, cortical layer, and cancellous substance. Staphylococcus aureus strain was used as an infectious agent. After the development of chronic osteomyelitis, the animals underwent osteonecrectomy. In the study group, autologous MSCs in Collatamp EG collagen carrier were placed into the bone defect. MSCs were obtained from adipose tissue and cultured in the matrix for 5 days. In control, the defect was filled with the collagen matrix without cells. Results. On day 14 upon the initiation of chronic osteomyelitis, bacteriological examination of the discharge from the fistula showed the presence of mixed bacterial flora (Staphylococcus aureus and Escherichia coli) in all operated animals. Results of X-ray, laboratory, and histological tests confirmed the formation of a focus of chronic osteomyelitis. Two months after the treatment (collagen with or without MSCs) began, all animals of the study group showed mature bone tissue regenerated in the affected zone. In the control group, proliferation of osteoblasts on the surface of the bone trabeculae was also observed; however, mature osteoid tissue was more often detected in the study group (35.0 vs 20.0% in control). In the study group (MSCs + collagen matrix), there was a decrease in bone marrow fibrosis (50.0 vs 100.0% in control) and cartilage formation (30.0 and 66.7%, respectively). After full treatment, newly formed bone trabeculae were detected more often (100.0 vs 60.0% in control); they were more mature and filled the defect area more efficiently. Conclusion. Our results indicate that the use of a collagen matrix with autologous MSCs is a promising plastic material for repairing osteomyelitic defects following necrectomy. The MSCs were able to increase the density of the filling material in the bone cavity, significantly accelerate the formation of bone beams around the matrix, and increase the tissue volume around the implant. The presence of MSCs significantly decreased the interference of a connective tissue component with osteogenesis and chondrogenesis.

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“…However, polysaccharides too like chitosan [112–114] or cornstarch [65, 115] have been investigated for this purpose. In between the animal-derived polymers, ASCs have demonstrated to positively interact mainly with fibrin [116], collagen [117, 118], gelatin [111, 119, 120], and silk [69, 121, 122], through the recognition of specific domains present in polymers' structures. These polymers have a good biodegradability profile, and they can be degraded, modified, or adsorbed by the action of naturally occurring enzymes [123].…”

Section: Scaffolds and Adipose Stem Cellsmentioning

confidence: 99%

Adipose-Derived Stem Cells in Bone Tissue Engineering: Useful Tools with New Applications

Storti

Scioli

Kim

et al. 2019

Stem Cells International

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Adipose stem cells (ASCs) are a crucial element in bone tissue engineering (BTE). They are easy to harvest and isolate, and they are available in significative quantities, thus offering a feasible and valid alternative to other sources of mesenchymal stem cells (MSCs), like bone marrow. Together with an advantageous proliferative and differentiative profile, they also offer a high paracrine activity through the secretion of several bioactive molecules (such as growth factors and miRNAs) via a sustained exosomal release which can exert efficient conditioning on the surrounding microenvironment. BTE relies on three key elements: (1) scaffold, (2) osteoprogenitor cells, and (3) bioactive factors. These elements have been thoroughly investigated over the years. The use of ASCs has offered significative new advancements in the efficacy of each of these elements. Notably, the phenotypic study of ASCs allowed discovering cell subpopulations, which have enhanced osteogenic and vasculogenic capacity. ASCs favored a better vascularization and integration of the scaffolds, while improvements in scaffolds' materials and design tried to exploit the osteogenic features of ASCs, thus reducing the need for external bioactive factors. At the same time, ASCs proved to be an incredible source of bioactive, proosteogenic factors that are released through their abundant exosome secretion. ASC exosomes can exert significant paracrine effects in the surroundings, even in the absence of the primary cells. These paracrine signals recruit progenitor cells from the host tissues and enhance regeneration. In this review, we will focus on the recent discoveries which have involved the use of ASCs in BTE. In particular, we are going to analyze the different ASCs' subpopulations, the interaction between ASCs and scaffolds, and the bioactive factors which are secreted by ASCs or can induce their osteogenic commitment. All these advancements are ultimately intended for a faster translational and clinical application of BTE.

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Repair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat model

Cited by 16 publications

References 16 publications

Isolation and Characterization of Buccal Fat Pad and Dental Pulp MSCs from the Same Donor

Isolation and Characterization of Buccal Fat Pad and Dental Pulp MSCs from the Same Donor

Technology for Repairing Osteomyelitic Bone Defects Using Autologous Mesenchymal Stromal Cells on a Collagen Matrix in Experiment

Adipose-Derived Stem Cells in Bone Tissue Engineering: Useful Tools with New Applications

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