Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment

Arias, Marcela Paola Castro; Maliza, Amanda Garcia Alves; Midena, Raquel Zanin; Graeff, Márcia Sirlene Zardin; Duarte, Marco Antônio Húngaro; Andrade, Flaviana Bombarda de

doi:10.1590/1678-775720150553

Cited by 19 publications

(24 citation statements)

References 33 publications

(49 reference statements)

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“…4 On the basis of the results of previous studies, the microbial communities in primary endodontic infections are more diverse than those in secondary endodontic infections. 5,6,7 Moreover, variation in the microorganisms isolated in several studies is likely a result of differences in the sampling techniques, laboratory procedures, and diagnostic approaches.…”

Section: 3mentioning

confidence: 99%

“…4 Understanding the microbiota composition in the infected root canal is essential for improving the treatment strategies to control infections and eliminate the pathogenic agents, as well as to prevent reinfection and periapical lesions. 9 The type and frequency of microbial agents involved in the genesis of endodontic diseases depend on several factors, such as geographic location, socioeconomic status, dietary habits, and oral hygiene status.…”

Section: Declaration Of Interestmentioning

confidence: 99%

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Culture-dependent approaches to explore the prevalence of root canal pathogens from endodontic infections

Pourhajibagher

Ghorbanzadeh²,

Bahador

2017

Braz. oral res.

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Endodontic infections are considered to be caused by the presence of various microorganisms within the root canal system. Recognition of this microbiota contributes to the successful treatment of infected root canals. This study investigated the microorganisms associated with primary and secondary endodontic infections via culture methods, biochemical tests, and molecular approaches in an Iranian population. Microbial specimens were collected from 36 patients with primary endodontic infection and 14 patients with a history of root canal therapy. Advanced microbiological culture techniques were used to isolate microbiota; subsequently, biochemical tests and 16S ribosomal RNA gene sequencing were performed to identify the microorganisms. Within the total 218 cultivable isolates, Veillonella parvula (20.6%) was found to occur with the highest frequency in primary endodontic infection, followed by Porphyromonas gingivalis (14.1%), and Aggregatibacter actinomycetemcomitans (9.2%). Enterococcus faecalis (36.6%) was the most predominant microorganism in secondary endodontic infections, followed by Candida albicans, Propionibacterium acnes, and V. parvula with frequencies of 20%, 2%, and 2%, respectively. It was concluded that V. parvula and E. faecalis was most frequently found in primary and secondary endodontic infections, respectively.

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Section: 3mentioning

confidence: 99%

Section: Declaration Of Interestmentioning

confidence: 99%

Culture-dependent approaches to explore the prevalence of root canal pathogens from endodontic infections

Pourhajibagher

Ghorbanzadeh²,

Bahador

2017

Braz. oral res.

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“…In each third, two images were obtained near the canal (superficial area) and two images at a distance from the canal (deep area), totalling 64 confocal images per each group (Arias et al . ) (Fig. ).…”

Section: Methodsmentioning

confidence: 93%

“…To analyse the dentinal tubules 8 sequential images were obtained in the cervical and middle thirds of each dentine cylinder; thus, there were four images in the cervical third and four images in the middle third. In each third, two images were obtained near the canal (superficial area) and two images at a distance from the canal (deep area), totalling 64 confocal images per each group (Arias et al 2016) (Fig. 2).…”

Section: Confocal Laser Scanning Microscopy (Clsm) Analysismentioning

confidence: 99%

Antimicrobial action of photodynamic therapy in root canals using LED curing light, curcumin and carbopol gel

et al. 2019

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Aim To evaluate the capacity of carbopol gel to maintain the intensity of a LED curing light (blueLED) along the length of prepared root canals in bovine teeth, and to assess the antimicrobial capacity of curcumin photoactivated by a LED curing light in the presence of carbopol gel. Methodology Experiment 1: Eight straight roots of bovine incisors were standardized to a length of 15 mm, and the root canals instrumented up to a size 120 K‐file. The LED curing light was irradiated inside the root canals using an aluminium collimator (1.5 mm in diameter) placed at the orifice (n = 8). Initially, the irradiation was performed in empty root canals and then repeated with the root canals filled with carbopol gel. Simple standardized photographs of the roots were taken with a digital camera in the mesial perspective during the irradiation procedure and the images analysed in OriginLab software to verify the light intensity along the length of the root. Experiment 2: Twenty dentine blocks were obtained from the cervical third of bovine incisors using a trephine bur. Biofilms were induced for 21 days on the blocks using Enterococcus faecalis (ATCC 4083) at 109 cells mL−1. The blocks were treated according to the groups (n = 5): positive control; standard PDT (methylene blue + diode Laser); curcumin; LED curing light; and curcumin + LED curing light. After the treatment, the samples were dyed with Live/Dead BacLight Bacterial Viability solution and fluorescence images were obtained by Confocal Scanning Laser Microscopy (CSLM). Experiment 3: Thirty‐two roots of bovine incisors were prepared as described in experiment 1. Their dentinal tubules were contaminated and the root canals treated according to the groups (n = 8): positive control; standard PDT; curcumin + LED curing light; curcumin + carbopol gel + LED curing light. The specimens were sectioned longitudinally and the split roots were treated with the Live/Dead dye to obtain fluorescence images by CSLM. All images were processed using BioImageL software to measure the percentage of viable bacteria and the data analysed statistically using the nonparametric Kruskal‐Wallis test (α < 0.05). Results In Experiment 1, carbopol gel did not improve the intensity of LED light transmission along the root canal. In Experiment 2, a significant decrease (P < 0.05) in bacterial viability occurred in the following order: positive control < only LED curing light < only curcumin < curcumin + LED curing light = standard PDT; and in Experiment 3 positive control = curcumin + LED curing light ≤ curcumin + gel + LED curing light ≤ standard PDT. Conclusion Similar disinfection effectiveness was obtained using curcumin + LED curing light and methylene blue + 660 nm LASER (standard PDT). The use of carbopol gel did not favour a greater transmission of LED light along the root canal and also resulted in less bacterial killing when used in endodontic PDT.

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“…Esses dados revelaram que a ativação ultrassônica da pasta de Ca(OH)2 promoveu uma maior redução do metabolismo bacteriano quando comparada à pasta não ativada. Esses dados estão de acordo com um estudo prévio in vitro que mostrou uma boa atividade da pasta de Ca(OH)2 ativada por ultrassom sobre infecção intra-tubular (Arias et al, 2016)…”

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Efeito da ativação ultrassônica da pasta de hidróxido de cálcio na atividade metabólica de bactérias em canais radiculares: Estudo clínico randomizado

Cazares¹

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AGRADECIMENTOSÀ Professora Doutora Ericka Tavares Pinheiro você é um excelente exemplo de pessoa e professora, que soube inspirar-me na realização do presente e futuros trabalhos, admiro muito sua dedicação e paciência, agradeço por compartilhar-me todos os seus conhecimentos e pelos ensinamentos para a vida, um agradecimento do fundo do coração. Muito Obrigado! À Professora Doutora Marcia Marques que com esforço, trabalho e dedicação soube deixar nos corações dos equatorianos que participaram no Dinter, uma gratidão enorme deixando ânsias de conquistas maiores. Aos professores do departamento de Dentística que participaram do DinterRenata Bommarito e Bárbara Guedes que foram fundamentais no desenvolvimento desta pesquisa, (grandes triagens, coleta de amostras, avaliação da dor, preservação e laboratório) sem duvida alguma cada procedimento foi realizado com sucesso por cada um de vocês. As boas amizades que ficam no Brasil, Lau, Lu, Alê, Carlos, Eduardo, Thais agradeço por tê-los conhecido, esses bons momentos e risos que ficaram sempre nas lembranças de este tempo maravilhoso. Tenham presente que no Equador vocês sempre se sentiram na sua casa. Agradeço aos colegas de Equador que foram parte do Dinter, em especial ao Klever, Frank, Kathy, Fabricio, Marcelo e Inês obrigado pelo convívio e apoio, e pela amizade pela oportunidade e por fazer possível nosso crescimento na pesquisa e conhecimento. Aos todos os funcionários da Clinica de pós-graduação, Departamento de Dentística e secretaria de Pós-graduação pelo apoio e colaboração."Tenho a impressão de ter sido uma criança brincando à beira-mar, divertindo-me em descobrir uma pedrinha mais lisa ou uma concha mais bonita que as outras, enquanto o imenso oceano da verdade continua misterioso diante de meus olhos". Isaac Newton RESUMORomero Cazares RX. Efeito da ativação ultrassônica da pasta de hidróxido de cálcio na atividade metabólica de bactérias em canais radiculares: Estudo clínico randomizado. São Paulo: Universidade de São Paulo, Faculdade de Odontologia; 2018. Versão Corregida. Protocolos complementares de desinfecção estão sendo investigados com o objetivo de promover uma maior redução de bactérias viáveis após o preparo químico-cirúrgico dos canais radiculares. Este estudo clínico randomizado comparou o efeito da ativação ultrassônica da pasta de hidróxido de cálcio com o da pasta não ativada na redução da atividade metabólica de bactérias nos canais radiculares. Vinte pacientes com necrose pulpar e periodontite apical assintomática foram alocados de forma randomizada em dois grupos de acordo com o protocolo de medicação intracanal: Grupo UA -ativação ultrassônica da pasta de hidróxido de cálcio (n=10) e Grupo NA -não ativação da pasta (n=10). Amostras microbiológicas dos canais radiculares foram coletadas após os procedimentos de: cirurgia de acesso (S1), preparo químicocirúrgico (S2), irrigação ultrassônica passiva (PUI) (S3) e medicação intracanal entre sessões com pasta de hidróxido de cálcio, com ou sem ativação ultrassônica (S4). As amostras endodônt...

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Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment

Cited by 19 publications

References 33 publications

Culture-dependent approaches to explore the prevalence of root canal pathogens from endodontic infections

Culture-dependent approaches to explore the prevalence of root canal pathogens from endodontic infections

Antimicrobial action of photodynamic therapy in root canals using LED curing light, curcumin and carbopol gel

Efeito da ativação ultrassônica da pasta de hidróxido de cálcio na atividade metabólica de bactérias em canais radiculares: Estudo clínico randomizado

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