Impact of micronutrients supplementation on bone repair around implants: microCT and counter-torque analysis in rats

Abstract

The use of natural substances and micronutritional approaches has been suggested as a therapeutic alternative to benefit the bone healing associated with no side effects. Nevertheless, the influence of micronutritional interventions with therapeutic proprieties on the bone repair has yet to be intensely evaluated, and no evidence is available exploring the impact of micronutrient supplementation on the peri-implant bone healing. Objective This study investigated the effect of micronutrients supplementation on the bone repair around implants.Material and Methods One screw-shaped titanium implant was inserted in each tibia of each rat, which were assigned to: daily administration, for 30 d, of the placebo solution (Placebo group-n:18) or micronutrients supplementation (Micronutrients group-n:18), based on calcium, magnesium, zinc, and vitamin D3 intake. After, the animals were sacrificed. One of the implants was removed by applying a counter-torque force to evaluate the force to rupture the bone-implant interface. The other implant was evaluated by microcomputed tomography (CT) examination to determine the bone-to-implant contact (BIC) and the bone volume (BV/TV).Results No statistically significant differences were observed between the groups for both counter-torque values and microCT parameters (p>0.05).Conclusion Within the limits of this study, micronutrients supplementation did not provide additional benefits to the bone healing around dental implants.

Resveratrol reverses the negative effect of smoking on peri‐implant repair in the tibia of rats

“…However, more studies should be conducted to confirm these results, given that this is the first one investigating the role of resveratrol daily use in the bone repair around implants in tabagism conditions, examining in vivo peri‐implant bone biopsies. Additionally, despite the fact that models of implant in the tibia have been widely used to research hypotheses regarding peri‐implant bone repair (Casarin et al, ; Correa et al, ; Ribeiro et al, ; Ribeiro, Nociti, Sallum, & Casati, ; Pimentel et al, ), they may not faithfully reproduce events in intra‐oral bone tissues and further investigations should be considered using oral models of dental implants.…”

“…Additionally, despite the fact that models of implant in the tibia have been widely used to research hypotheses regarding peri-implant bone repair (Casarin et al, 2014;Correa et al, 2009;Ribeiro et al, 2006;Ribeiro, Nociti, Sallum, & Casati, 2009;Pimentel et al, 2016), they may not faithfully reproduce events in intra-oral bone tissues and further investigations should be considered using oral models of dental implants.…”

“…A wrench was adapted to the implant head to apply torque in the reverse direction of implant placement until complete rupture of the bone–implant interface was signaled by rotation of the implant. The torque force value achieved in N/cm was considered as the torque necessary for the breakdown of osseointegration (Pimentel et al, ).…”

Do Dietary Supplements and Nutraceuticals Have Effects on Dental Implant Osseointegration? A Scoping Review

“…Pimentel et al, in 2016 [44], evaluated the effects of a mixture of calcium, magnesium, zinc, and vitamin D on rats that received titanium implants. They found that there was no statistically significant difference among the counter-torque values for implant removal, bone volume, and BIC in the placebo group when compared to the micronutrient group.…”

Vitamin D and Fracture Healing: An Ongoing Puzzle

“…Here again, however, contrary results include those of Sun et al [52] who found vitamin D binding protein had no effect on enhancing healing in rat bone defects. Vitamin D can also increase the state of bone resorption, or raise the risk for further fractures or negative healing outcomes [22] , may not foster better implant healing [53] , and may well hamper the initial pro-inflammatory state that occurs postfracture as indicated by Wasnik et al [54] .…”