Antimicrobial activity of complete denture cleanser solutions based on sodium hypochlorite and Ricinus communis – a randomized clinical study

Abstract

To preserve oral health and to maintain the prosthetic devices, it is important not only to improve the properties of commonly known hygiene products, but also to investigate new materials with antimicrobial action. Objectives This study evaluated the antimicrobial activity of sodium hypochlorite (0.25% and 0.50%) and 10% Ricinus communis’ solutions against specific microorganisms.Material and Methods Sixty four maxillary complete denture wearers were instructed to brush their dentures three times a day and to soak them (20 min/day) in the solutions: SH1: 0.25% sodium hypochlorite; SH2: 0.5% sodium hypochlorite; RC: 10% R. communis oil; and C: 0.85% saline (control). The solutions were used for 7 days in a randomized sequence. Following each period of use, there was a 1-week washout period. Antimicrobial activity was determined by Colony Forming Units (CFU) counts of Streptococcus mutans, Candida spp., and gram-negative microorganisms. For collecting biofilm, the internal surface of maxillary dentures was brushed with saline solution, and biofilm suspension obtained. After dilutions (100 - 10-3), aliquots were seeded in Mitis salivarius, CHROMagar Candida®, and MacConkey agar for detecting S. mutans, Candida spp., or gram-negative microorganisms, respectively. After incubation, colonies were counted, and CFU/mL values were calculated. Then, transformation - log10 (CFU+1) - data were analyzed using the Friedman test (α=0.05). Results showed significant differences between the solutions (p<0.001).Results All three solutions showed antimicrobial activity against S. mutans. Against Candida spp., RC and SH1 solutions showed similar effect while SH2 showed superior activity. SH1 and SH2 solutions showed antimicrobial action against gram-negative microorganisms. The Candida species most frequently isolated was C. albicans, followed by C. tropicalis and C. glabrata.Conclusions The 0.5% sodium hypochlorite solution was the most effective and might be used to control denture biofilm. C. albicans was the most frequently isolated Candida sp.

Clinical trial for evaluation of Ricinus communis and sodium hypochlorite as denture cleanser

“…Correct denture hygiene is essential to reduce or eliminate pathogens 15 , 22 , 23 and establish an environment suitable for the growth of beneficial oral microbiota. Studies have shown that a combination of mechanical and chemical cleaning is efficient in the maintenance of denture hygiene 7 , 22 .…”

“…Studies have shown that a combination of mechanical and chemical cleaning is efficient in the maintenance of denture hygiene 7 , 22 .…”

Protez Temi̇zleyi̇ci̇ Solüsyonlarin Isi İle Poli̇meri̇ze Olan Akri̇li̇k Rezi̇ni̇n Mi̇krosertli̇ği̇ne Etki̇si̇

“…12 Sodyum hipoklorit protez temizliğinde rutin olarak tavsiye edilen kimyasal bir solüs-yondur. 11 Etkili bir dezenfektan ajan olmasına rağmen akrilik rezinde beyazlamaya ve protezin metal komponentlerinde korozyona neden olması kullanımlarını kısıtlar. [11][12][13][14] Tablet veya toz halinde ticari formları bulunan alkalin peroksitler oksidan, efervesan, yüzey gerilimi azaltıcı ve şelasyon ajanından oluşurlar ve su ile temasa geçince hidrojen peroksit solüsyonu haline gelirler.…”

Effect of Alkaline Peroxide-type Denture Cleaners to the Microbial Profile in Maxillary Complete Denture

“…Few microorganisms have been identified to contribute to the infection, namely Streptococcus, Staphylococcus, Candida, Neisseria, Lactobacillus, and Actinomyces. 3,5,7 Recent studies have reported coadhesion between Candida and Streptococcus species, which promotes oral colonization by yeast cells that leads to denture stomatitis. 3,6 Denture hygiene is important in reducing the amount of denture biofilm, as well as the incidence of denture stomatitis.…”

“…A combination of both methods has shown effective results in reducing the amount of microorganisms present. [2][3][4]7,8 Chemical methods may be classified into four groups, based on the mechanism of action or main ingredients of cleansers, namely sodium hypochlorites, alkaline peroxides, mouth rinses and enzymes. 3,4 Alkaline peroxide solutions are widely indicated for controlling biofilm; however, the efficacy of such agents remains inconclusive.…”