Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats

Tera, Tábata de Mello; Nascimento, Rodrigo Dias; Prado, Renata Falchete do; Santamaría, Mauro Pedrine; Jardini, Maria Aparecida Neves

doi:10.1590/1678-775720140190

Cited by 21 publications

(21 citation statements)

References 30 publications

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“…ON staining of osteocytes was not observed in the ovariectomized or nonovariectomized groups in our study, and the ON staining of osteoblasts was moderate. The results of studies by Tera et al [52] and Ishigaki et al [54] were similar to those of our study. In the literature, many immunohistochemical and histological studies have shown that rhBMP-2 is effective in increasing new bone formation [18,31,40,[55][56][57].…”

Section: Discussionsupporting

confidence: 93%

“…Ivanovski et al observed that OC staining of osteoblasts and osteocytes was strong in the graft group [53]. Another study showed that osteocyte staining was moderate in both the ovariectomized and nonovariectomized groups [54]. In the present study, OC staining of osteoblasts was moderate, and no staining of osteocytes was observed in either the ovariectomized or nonovariectomized groups.…”

Section: Discussionsupporting

confidence: 50%

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Evaluation of the effects of bone morphogenetic protein-2 on the healing of bone calvarial defects in ovariectomized rats

Kadiroğlu¹,

Karayürek²,

Akbalık³

2020

Turk J Vet Anim Sci

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Introduction Inadequate bone support for oral surgery and periodontal regeneration is a major problem for surgical applications. Additional graft materials are needed in dentistry to repair missing bone tissue. Currently, due to their osteogenic, osteoinductive, and osteoconductive effects, autogenous bone grafts are considered the gold standard under certain conditions [1]. However, other conditions, such as the presence of a second surgical site and the failure to obtain the desired amount of material, limit the use of autogenous bone grafts [2]. Because of this, many researchers have attempted to find an alternative to autografts. For instance, allografts have been used in dentistry for a long time. Allografts have osteoconductive and osteoinductive effects [3]. Regrettably, the devitalization process used to suppress the host immune response and prevent possible disease transmission in cadaveric allografts causes the loss of the mechanical, osteoinductive, and osteoconductive properties of the graft [4,5]. In addition, the maturation times of allografts are longer in defect regions than those of autografts [6,7]. Bone morphogenetic proteins (BMPs) are well known to induce osteogenic effects. Allografts typically contain BMPs that accelerate mesenchymal cell migration and attachment [3]. In both in vitro and in vivo studies of BMP-2, which is a type of BMP, the contribution of BMP-2 to ossification could be shown. BMP-2 promotes periodontal and bone regeneration in some animal models [8,9] and also induces differentiation of preosteoblast cells and thereby increases ossification [10,11]. Fundamentally, BMPs are types of growth factors and belong to the transforming growth factor (TGF-β) family [12]. There are variant BMPs that support bone regeneration, such as BMP-2,-4,-5,-6, and-7 [13]. Recombinant human bone morphogenetic protein-2 (rhBMP-2), generated by recombinant DNA technology from mammalian cells, is known to induce differentiation of osteoblasts at a cellular level and to induce osteogenesis in animal models [14]. Bone graft materials and osteoinductive agents have a synergistic effect [15]. The synergistic effect of BMPs and bone grafts on bone regeneration has been demonstrated in animal studies [11].

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 50%

Evaluation of the effects of bone morphogenetic protein-2 on the healing of bone calvarial defects in ovariectomized rats

Kadiroğlu¹,

Karayürek²,

Akbalık³

2020

Turk J Vet Anim Sci

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“…(animals with cranial defect with MSCs, ovariectomy group) and C2 (animals with cranial defect with MSCs, control group). Thus, estrogen deficiency may not influence the expression of bone markers, which is consistent with the findings of Tera Tde et al (36). According to the results of this study, more new bone formation was observed in defects treated with MSCs alone than was observed in animals with cranial defect only.…”

Section: Discussionsupporting

confidence: 92%

“…Research suggests it plays a role in attaching osteoclasts to the mineral matrix of bones and in the regulation of normal mineralization within the extracellular matrices of bones and teeth. Osteocalcin and osteonectin are not observed during initial crystal formation but are seen in the later stages of bone formation (36). Therefore, we chose these three bone markers to assess the bone formation activity.…”

Section: Discussionmentioning

confidence: 99%

Calvarial bone defects in ovariectomised rats treated with mesenchymal stem cells and demineralised freeze-dried bone allografts

et al. 2020

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The aim of the study was to investigate the ability of a combination of bone marrow mesenchymal stem cells (BM-MSCs) with and without demineralized freeze-dried bone allografts (DFDBAs) to induce bone regeneration in calvarial defects in ovariectomized rats.Critical size defects were filled with a combination of demineralized freeze-dried bone allografts and bone marrow mesenchymal stem cells (BM-MSCs) or BM-MSCs alone. Eight weeks after calvarial surgery, the rats were sacrificed. The samples were analyzed histologically and immunohistochemically. No difference was observed in vascularization between groups C1 (animals with cranial defect only, control group) and O1 (animals with cranial defect only, ovariectomy group). Intramembranous ossification was observed at a limited level in groups C2 (animals with cranial defect with MSCs, control group) and O2 (animals with cranial defect with MSCs, ovariectomy group) compared to C1 and O1. In group C3 (animals with demineralized freeze-dried bone allografts with MSCs, control group), the fibrous structures of the matrix became compact as a result of a bone graft having been placed in the cavity, but in group O3 (animals with demineralized freeze-dried bone allografts with MSCs, ovariectomy group), the fibrous tissue was poorly distributed between the bone grafts for the most parts. We conclude that the insertion of BM-MSCs enhances bone healing; however, the DFDBA/BM-MSC combination has little effect on overcoming impaired bone formation in ovariectomized rats. Key words: bone healing, bone marrow mesenchymal stem cells (BM-MSCs),demineralized freeze-dried bone allografts (DFDBAs), ovariectomy, calvarial defect

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“…The use of biomarkers and imaging techniques are important to evaluate the biological stages of bone repair (Hankenson, Zimmerman, & Marcucio, ). Among the used biomarkers are osteocalcin, a strong marker of bone formation, sialoprotein, a more intense marker in newly formed bone, VEGF, VEGF‐R2, and CD‐31, markers associated with angiogenesis (Chen et al, ; Coates, ; Di Domenico et al, ; Esteves et al, ; Hankenson et al, ; Street et al, ; Tera, Nascimento, do Prado, Santamaria, & Jardini, ).…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of rhBMP‐2 association to autogenous, allogeneic, and heterologous bone grafts

Gonzaga

Kotake

Figueiredo

et al. 2019

Microscopy Res & Technique

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Proteins with osteoinductive potential, especially recombinant human bone morphogenetic protein (rhBMP)‐2, have large effects on cell growth and their differentiation. The aim of this study was to assess repair of bone defects in rat calvaria with different types of grafts associated with rhBMP‐2, through immunohistochemistry and micro computed tomography (CT) analyses. A total of 35 male Wistar rats were selected, each weighing ~250 g, with a waiting period of 6 weeks from the creation of the defect to the sacrifice, and divided into five groups (n = 7): autograft plus 5 μg rhBMP‐2 (AuG/BMP‐2); allograft plus 5 μg rhBMP‐2 (AlG/BMP‐2); xenograft (heterologous) plus 5 μg rhBMP‐2 (XeG/BMP‐2); 5 μg rhBMP‐2 (BMP‐2) and the control group (n = 7). The micro CT reveal that all groups associating different bone grafts with BMP‐2 showed increased bone formation compared to the control. The immunostaining show that osteocalcin and bone sialoprotein were higher in groups with BMP‐2 than control group; BMP was high expressed in AuG/BMP‐2, AlG/BMP‐2, and BMP‐2; vascular endothelial growth factor (VEGF) was more expressed in groups with BMP‐2; VEGF‐R2 was low to moderate in AuG/BMP‐2, XeG/BMP‐2, and BMP‐2, predominantly moderate in AlG/BMP‐2 and low in the control; CD‐31 was predominantly moderate in AuG/BMP‐2, AlG/BMP‐2, and XeG/BMP‐2, low to moderate in BMP‐2 and low in the control. The results revealed that rhBMP‐2 improved bone repair when administered alone, or when associated with different bone grafts.

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Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats

Cited by 21 publications

References 30 publications

Evaluation of the effects of bone morphogenetic protein-2 on the healing of bone calvarial defects in ovariectomized rats

Evaluation of the effects of bone morphogenetic protein-2 on the healing of bone calvarial defects in ovariectomized rats

Calvarial bone defects in ovariectomised rats treated with mesenchymal stem cells and demineralised freeze-dried bone allografts

Effectiveness of rhBMP‐2 association to autogenous, allogeneic, and heterologous bone grafts

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