Osteopontin expression in reactive lesions of gingiva

Abstract

Reactive proliferations of the gingiva comprise lesions such as pyogenic granuloma (PG), inflammatory fibroepithelial hyperplasia (IFH), peripheral ossifying fibroma (POF), and peripheral giant cell lesion. Osteopontin (OPN) has a dual role, it promotes mineralization when it is bound to solid substrate, and on the other hand, it inhibits mineralization when it is seen in association with solution.Objectives The study aimed to evaluate the expression of osteopontin in normal gingival tissue and different types of focal reactive proliferations of gingival tissue, and its role in the development of calcification within it.Material and Methods The presence and distribution of osteopontin was assessed using immunohistochemistry in five cases of normal gingival tissue and 30 cases of focal reactive proliferations of gingiva.Results There was no expression of osteopontin in normal subjects. Few cases of pyogenic granuloma, inflammatory fibroepithelial hyperplasia, and all the cases of peripheral ossifying fibroma showed positivity for osteopontin in the inflammatory cells, stromal cells, extracellular matrix, and in the calcifications.Conclusion The expression of osteopontin in all the cases of peripheral ossifying fibroma speculates that the majority of the cases of peripheral ossifying fibroma originate from the periodontal ligament cells. The treatment modalities for peripheral ossifying fibroma should differ from other focal reactive proliferations of gingiva.

Human gingiva transcriptome during wound healing

“…), reactive gingival lesions (Elanagai et al. ), and other oral mucosal diseases (Danielsson et al. ).…”

Effects of deferoxamine on the osteogenic differentiation of human periodontal ligament cells

“…OPN produced by osteoblasts at various stages of differentiation is one of the vital non-collagenous bone matrix proteins. High OPN calcium-binding potential can promote calcium adhesion, thus influencing mineralization events (42). Col-1, an early marker of osteogenic differentiation, is a major extracellular matrix component of periodontal tissues that promotes osteoblast differentiation and mineralization (43).…”

Diagnostic and Biological Significance of Immunohistochemical Expression of Osteopontin and Ki67 in Fibro-osseous lesions of jaws

“…Also, formation and structure of the hard tissue in OF share more similarities with normal bone and cementum, and this could be another probable reason for higher expression of OPN in OF. Moreover, some studies demonstrated higher expression of OPN in peripheral OF compared to other reactive lesions which are not related initially to periodontal ligament [37,38]. Also, it could explain its overexpression in FD of the jaws compared to osteofibrous dysplasia, a FOS of long bones.…”