Rat subcutaneous tissue response to calcium silicate containing different arsenic concentrations

Abstract

Objective To evaluate the response of rat subcutaneous tissue in implanted polyethylene tubes that were filled with GMTA Angelus and Portland cements containing different arsenic concentrations.Material and Methods Atomic absorption spectrophotometry was utilized to obtain the values of the arsenic concentration in the materials. Thirty-six rats were divided into 3 groups of 12 animals for each experimental period. Each animal received two implants of polyethylene tubes filled with different test cements and the lateral of the tubes was used as a control group. After 15, 30 and 60 days of implantation, the animals were killed and the specimens were prepared for descriptive and morphometric analysis considering: inflammatory cells, collagen fibers, fibroblasts, blood vessels and other components. The results were analyzed utilizing the Kuskal-Wallis test and the Dunn´s Multiple test for comparison (p<0.05).Results The materials showed, according to atomic absorption spectrophotometry, the following doses of arsenic: GMTA Angelus: 5.01 mg/kg, WPC Irajazinho: 0.69 mg/kg, GPC Minetti: 18.46 mg/kg and GPC Votoran: 10.76 mg/kg. In a 60-day periods, all specimens displayed a neoformation of connective tissue with a structure of fibrocellular aspect (capsule). Control groups and MTA Angelus produced the lower amount of inflammatory reaction and GPC Minetti, the highest reaction.Conclusions There was no direct relationship between the concentration of arsenic present in the composition of the materials and the intensity of the inflammatory reactions. Higher values, as 18.46 mg/kg of arsenic in the cement, produce characteristics of severe inflammation reaction at the 60-day period. The best results were found in MTA angelus.

Systemic effect of mineral aggregate-based cements: histopathological analysis in rats

“…In the case of arsenic, the dose considered lethal ranges from 2 to 3 mg/Kg of body weight. Thus, 140 to 210 mg of arsenic would be required to poison an individual weighing 70 Kg 23 . Considering that the quantity of MTA used during a clinical procedure is less than 1.0 g, the quantity of arsenic present in the cement is much lower than the lethal dose 23 .…”

“…Thus, 140 to 210 mg of arsenic would be required to poison an individual weighing 70 Kg 23 . Considering that the quantity of MTA used during a clinical procedure is less than 1.0 g, the quantity of arsenic present in the cement is much lower than the lethal dose 23 . However, from the clinical point of view, despite the low levels of arsenic released by the cements 12 , the quantity necessary to cause significant changes in tissues and vital organs that are at a certain distance from the site of application has not yet been elucidated.…”

‘Effects of novel root repair materials on attachment and morphological behaviour of periodontal ligament fibroblasts: Scanning electron microscopy observation’

“…However, arsenic was detected in the chemical composition of Biodentine, at a high ratio (9.26%), in the present study. Recent animal research evaluating tissue responses to different calcium silicate based materials has revealed that the arsenic concentration did not directly affect the intensity of the inflammatory reactions (Minotti et al, ). Therefore, the high concentration of arsenic in Biodentine does not seem to negatively influence cell adhesion.…”

Analysis of the reaction of subcutaneous tissues in rats and the antimicrobial activity of calcium hydroxide paste used in association with different substances

“…Quantitative analysis using an optical microscope (Aristoplan, Leitz Wetzlar, Germany) with a 100× objective and 8× Zeiss Kpl eyepiece containing a Zeiss II integration grid with 100 points symmetrically distributed over a quadrangular area, determined the volume density occupied by the inflammatory infiltrate (giant cells, polymorphonuclear cells and mononuclear cells), fibroblasts, collagen fibers and blood vessels 19 , 21 .…”