Non-cross-linked collagen type I/III materials enhance cell proliferation: in vitro and in vivo evidence

Abstract

ObjectiveTo analyze Mucograft®(MG), a recently introduced collagen matrix, in vitro and in vivo, and compare it with BioGide®(BG), a well-established collagen membrane, as control. Material and MethodsA detailed analysis of the materials surface and ultra-structure was performed. Cellular growth patterns and proliferation rates of human fibroblasts on MG and BG were analyzed in vitro. In addition, the early tissue reaction of CD-1 mouse to these materials was analyzed by means of histological and histomorphometrical analysis. ResultsMG showed a three-fold higher thickness both in dry and wet conditions, when compared to BG. The spongy surface of BG significantly differed from that of MG. Cells showed a characteristic proliferation pattern on the different materials in vitro. Fibroblasts tended to proliferate on the compact layers of both collagens, with the highest values on the compact side of BG. In vivo, at day three both materials demonstrated good tissue integration, with a mononuclear cell sheet of fibroblasts on all surfaces, however, without penetrating into the materials.ConclusionsThe findings of this study showed that MG and BG facilitate cell proliferation on both of their surfaces in vitro. In vivo, these two materials induce a comparable early tissue reaction, while serving as cell occlusive barriers.

Long‐term outcomes after vestibuloplasty with a porcine collagen matrix (Mucograft®) versus the free gingival graft: a comparative prospective clinical trial

“…Due to its excellent hydrophilicity, the matrix hydrates very quickly with the patient's blood after placement in the defect. In vivo tests demonstrate good tissue integration, with a mononuclear cell sheet of fibroblasts on the surface after 3 days, promoting further soft tissue healing (Willershausen et al 2014).…”

“…In vivo tests demonstrate good tissue integration, with a mononuclear cell sheet of fibroblasts on the surface after 3 days, promoting further soft tissue healing (Willershausen et al. ).…”

In vitro observation of macrophage polarization and gingival fibroblast behavior on three‐dimensional xenogeneic collagen matrixes

“…A direct culture test of the CMs showed that ncl_CM resulted in reduced cell viability compared to the control and cl_CM (Figure c). Previous in vitro studies reported that ncl_CM showed noncytotoxic properties in human gingival fibroblasts (Lima et al, ; Willershausen et al, ) when compared with those of polystyrene (Lima et al, ) or a collagen barrier membrane (BioGide®, Geistlich) (Willershausen et al, ). Our results showed reduced viability of cells cultured on ncl_CM, which might be because different sizes of CM were tested; the size of 3 × 2 mm was used in the study by Lima et al (Lima et al, ), while the size of 5 × 5 mm was used in the present study performed in 96‐well plates.…”

Hyaluronic acid slows down collagen membrane degradation in uncontrolled diabetic rats

“…5 Collagen membrane resorption occurs through a biodegradation process which starts when cells within the surgical site release matrix metalloproteinases (MMPs) into the wound area during healing and continues by fibroblast and blood vessel infiltration and colonization, leading to membrane degradation. [5][6][7] It has been shown that collagen membrane stability plays a crucial role in the success of regenerative procedures. [8][9][10][11] Premature exposure to the oral cavity is leading to early degradation and bacterial colonization compromising the beneficial effects of GTR or GBR procedures.…”

“…Membranes create and maintain the space over the bony defect and act as a barrier against the ingrowth of epithelial and connective tissue cells into the defect . Collagen membrane resorption occurs through a biodegradation process which starts when cells within the surgical site release matrix metalloproteinases (MMPs) into the wound area during healing and continues by fibroblast and blood vessel infiltration and colonization, leading to membrane degradation . It has been shown that collagen membrane stability plays a crucial role in the success of regenerative procedures .…”