Catechin-incorporated dental copolymers inhibit growth of Streptococcus mutans

Abstract

Objective:To test the inhibitory growth activity of green tea catechin incorporated into dental resins compared to resins containing the broad-spectrum antimicrobial compound chlorhexidine against Streptococcus mutans in vitro.Material and Methods:The minimum inhibitory concentrations (MICs) of epigallocatechin-gallate (EGCg) and chlorhexidine (CHX) were determined according to the microdilution method. Resin discs (5 mm x 3 mm) were prepared from Bis-GMA/TEGDMA (R1) and Bis-GMA/CH3Bis-GMA (R2) comonomers (n=9) containing: a) no drug, b) EGCg, c) CHX. Two concentrations of each drug (0.5x MIC and 1x MIC) were incorporated into the resin discs. Samples were individually immersed in a bacterial culture and incubated for 24 h at 37º C under constant agitation. Cell viability was assessed by counting the number of colonies on replica agar plates. Statistical analysis was performed using one-way ANOVA, Tukey and Student t-tests (α=0.05).Results:Both resins containing EGCg and CHX showed a significant inhibition of bacterial growth at both concentrations tested (p<0.05). A significantly higher inhibition was observed in response to resins containing CHX at 0.5x MIC and 1x MIC, and EGCg at 1x MIC when compared to EGCg at 0.5x MIC. Also, EGCg at 0.5x MIC in R1 had a significantly higher growth inhibition than in R2.Conclusions:Both EGCg and CHX retained their antibacterial activity when incorporated into the resin matrix. EGCg at 1x MIC in R1 and R2 resins significantly reduced S. mutans survival at a level similar to CHX. The data generated from this study will provide advances in the field of bioactive dental materials with the potential of improving the lifespan of resin-based restorations.

Antibacterial Efficacy of Epigallocatechin-3-gallate against Streptococcus mutans: A Systematic Review / Eficacia antibacteriana de la epigalocatequina-3-galato contra el Streptococcus mutans: Revisión Sistemática

“…CHX presents antimicrobial efficacy in vitro and in vivo (mouthwash) against salivary microbiota cariogenic and has a role in preventing the development of gingivitis [22]. Some studies compared EGCG with CHX [17,23,24,25,26]. e results of the comparison between CHX and EGCG showed that both substances were effective in inhibiting microbial the growth and biolm formation.…”

Characterization of an experimental resin composite organic matrix based on a tri-functional methacrylate monomer

“…These findings are supported by previous studies and highlight the influence of polar, hydrophilic -OH groups on absorption. Sideridou et al 11) showed that poly-Bis-GMA presented higher water absorption than did poly-Bis-EMA and claimed that this was due to the higher cohesive energy density for hydrogen bonding of -OH (2,980 J/cm 3 ) when compared to the -O-group present in Bis-EMA (881 J/cm 3 ). Additionally, when analyzing three resin composites, Örtengren et al 27) showed that the absorption of composites with Bis-GMA and TEGDMA in their matrixes was comparatively higher than a composite with Bis-EMA, i.e.…”

“…After every 24 h period, the discs were weighed (AUW 220, Shimadzu, Tokyo, Japan) until a constant mass (m 1) was attained, i.e., disc mass variation was less than ±0.01 mg in any 24 h period. The thickness and the diameter of the discs were measured using a digital caliper (MPI/E-101, Mitutoyo, Tokyo, Japan), and the volume (V ) was calculated in mm 3 . The discs were then individually placed in plastic vials and immersed in 20 mL of distilled water at 37±1ºC.…”

“…Since the development of the resin composites by Rafael BOWEN in the late 1950s, many efforts have been made to improve the behavior of this class of restorative materials [1][2][3] . In general, resin composites comprise three phases: a organic matrix formed by a mix of bi-functional methacrylate monomers, inorganic filler particles and a silane-coupling agent that chemically bonds the organic matrix to the filler particles.…”

Formulação à base de Epigalocatequina-3-galato, derivada do chá verde, desenvolvida para uso endodôntico: estudo físico-químico e biológico

“…Estudos mostram que essa substância desempenha efeito anticariogênico decorrente de sua efetiva ação antimicrobiana contra S. mutans e S. sobrinus (Sakanaka et al, 1989;Yoshino et al, 1996;Ferrazzano et al, 2011;Tamura et al, 2011;Xu et al, 2012;Shumi et al, 2014;Anita et al, 2015;Sarin et al, 2015), por impedir a aderência bacteriana ao dente por meio da inibição da enzima glicosiltransferase e da amilase bacteriana (Hamilton-Miller, 2001;Subramaniam et al, 2012;Jung et al, 2012;Cai et al, 2013). Devido à sua comprovada eficácia contra os micro-organismos e na inibição de metaloproteinases, esse extrato tem sido incorporado em materiais odontológicos, tais como, adesivos dentinários (Du et al, 2012), resinas compostas Mankovskaia et al, 2013) e cimentos de ionômero de vidro (Hu et al, 2013). Além disso, seu uso tem sido sugerido na redução da erosão e abrasão dentária (Magalhães et al, 2009;Kato et al, 2010), em solução para bochechos (Hirasawa et al, 2006;Jenabian et al, 2012;Neturi et al, 2014;Sarin et al, 2015), em dentifrícios (Maruyama et al, 2011;Hrishi et al, 2015) e como meio para a conservação de dentes avulsionados após traumatismos, mantendo a vitalidade das células do ligamento periodontal (Hwang et al, 2011;Jung et al, 2011;Poi et al, 2013;Ghasempour et al, 2015).…”