Metabolic effect of low fluoride levels in the islets of NOD mice: integrative morphological, immunohistochemical, and proteomic analyses

Trevizol, Juliana Sanches; Dionízio, Aline; Delgado, Aislan Quintiliano; Ventura, Talita Mendes Oliveira; Ribeiro, Camila Nunes de Morais; Ribeiro, Lucas F.; Buzalaf, Nathalia Rabelo; Cestari, Tânia Mary; Magalhães, Ana Carolina; Suzuki, Maiko; Bosqueiro, José Roberto; Buzalaf, Marília Afonso Rabelo

doi:10.1590/1678-7757-2023-0036

J. Appl. Oral Sci.

2023

DOI: 10.1590/1678-7757-2023-0036

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Metabolic effect of low fluoride levels in the islets of NOD mice: integrative morphological, immunohistochemical, and proteomic analyses

Juliana Sanches Trevizol

Aline Dionízio

Aislan Quintiliano Delgado

et al.

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Cited by 3 publications

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Adaptive responses of the ileum of NOD mice to low‐dose fluoride: A proteomic exploratory study

Trevizol,

Buzalaf,

Dionizio

et al. 2024

Cell Biochemistry & Function

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Fluoride (F) has been employed worldwide to control dental caries. More recently, it has been suggested that the consumption of low doses of F in the drinking water may reduce blood glucose levels, introducing a new perspective for the use of F for the management of blood glucose. However, the exact mechanism by which F affects blood glucose levels remains largely unexplored. Given that the small gut plays a pivotal role in glucose homeostasis, the aim of this study was to investigate the proteomic changes induced by low doses of F in the ileum of female nonobese‐diabetic (NOD) mice. Forty‐two female NOD mice were divided into two groups based on the F concentration in their drinking water for 14 weeks: 0 (control) or 10 mgF/L. At the end of the experimental period, the ileum was collected for proteomic and Western blot analyses. Proteomic analysis indicated an increase in isoforms of actin, gastrotropin, several H2B histones, and enzymes involved in antioxidant processes, as well as a decrease in enzymes essential for energy metabolism. In summary, our data indicates an adaptive response of organism to preserve protein synthesis in the ileum, despite significant alterations in energy metabolism typically induced by F, therefore highlighting the safety of controlled fluoridation in water supplies.

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Adaptive responses of the ileum of NOD mice to low‐dose fluoride: A proteomic exploratory study

Trevizol,

Buzalaf,

Dionizio

et al. 2024

Cell Biochemistry & Function

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The fluorosis conundrum: bridging the gap between science and public health

Kumar,

Chhabra,

Mehra

et al. 2023

Toxicology Mechanisms and Methods

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Optimized protocol for shotgun label-free proteomic analysis of pancreatic islets

SanchesTrevizol,

Dionizio,

Delgado

et al. 2024

Biology Methods and Protocols

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Pancreatic islets are crucial in diabetes research. Consequently, this protocol aims at optimizing both the protein-extraction process and the proteomic analysis via shotgun methods for pancreatic islets. Six protocols were tested, combining three types of chemical extraction with two mechanical-extraction methods. Furthermore, two protocols incorporated a surfactant to enhance enzymatic cleavage. The steps involved extraction and concentration of protein, protein quantification, reduction, alkylation, digestion, purification and desalination, sample concentration to ∼ 1 µL and proteomic analysis using the mass spectrometer. The most effective protocol involves either a milder chemical extraction paired with a more intensive mechanical process, or a more robust chemical extraction paired with a gentle mechanical process, tailored to the samplés characteristics. Additionally, it was observed that the use of a surfactant proved ineffective for these types of samples. Protocol 5 was recently used with success to examine metabolic changes in pancreatic islets of NOD mice exposed to low doses of fluoride ions (F-) and the primary pathways altered by the treatment.

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Metabolic effect of low fluoride levels in the islets of NOD mice: integrative morphological, immunohistochemical, and proteomic analyses

Cited by 3 publications

References 41 publications

Adaptive responses of the ileum of NOD mice to low‐dose fluoride: A proteomic exploratory study

Adaptive responses of the ileum of NOD mice to low‐dose fluoride: A proteomic exploratory study

The fluorosis conundrum: bridging the gap between science and public health

Optimized protocol for shotgun label-free proteomic analysis of pancreatic islets

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