Characterization of white spot lesions formed on human enamel under microcosm biofilm for different experimental periods

Abstract

Characterization of white spot lesions formed on human enamel under microcosm biofilm for different experimental periodsThe initial characteristics of white spot lesion (WSLs), such as the degree of integrated mineral loss (ΔZ), depth and pattern of mineral distribution, have an impact on further demineralization and remineralization. However, these lesion parameters have not been evaluated in WSLs produced from microcosm biofilms. Objective: This study characterized artificial white spot lesions produced on human enamel under microcosm biofilm for different experimental periods. Methodology: In total, 100 human enamel specimens (4x4mm) were assigned to 5 distinct groups (n=20/group) differing according to the period of biofilm formation (2, 4, 6, 8 or 10 days). Microcosm biofilm was produced on the specimens from a mixture of human and McBain saliva at the first 8h. Enamel samples were then exposed to McBain saliva containing 0.2% sucrose. WSLs formed were characterized by quantitative light-induced fluorescence (QLF) and transverse microradiography (TMR). Data were analyzed by ANOVA/Tukey or Kruskal-Wallis/Dunn tests (p<0.05). Results: A clear timeresponse pattern was observed for both analyses, but TMR was able to better discriminate among the lesions. Regarding QLF analysis, median (95%CI; %) changes in fluorescence ∆Z were -7.

Magnetic‐based photosensitizer to improve the efficiency of antimicrobial photodynamic therapy against mature dental caries‐related biofilms

“…The biofilm age also presents relevance for caries progression. 26 Teeth covered with mature biofilms are exposed to lactic acid, causing the calcium phosphate of the tooth to dissolve, a process called demineralization, in which lesions and cavities in the teeth are created. 27 Mature biofilms are more acidogenic and less susceptible to destruction and decomposition.…”

Comparison of biofilm models for producing artificial active white spot lesions

“…In this context, in vitro models that employ cariogenic biofilms for long periods of time and under controlled laboratorial environment to create artificial lesions have been prioritized. 7 - 9 These biofilm models are an excellent alternative for mimicking the natural condition of the oral cavity, studying biofilm formation, creating caries lesions, and developing and testing new therapies to arrest caries lesion progression. 10 - 12 On the other hand, in vitro biofilm models also use a single strain, mainly S. mutans, within a pure culture system that leads to a mono-species biofilm.…”

“… 13 In vivo cariogenic biofilms are composed of numerous strains that cannot be readily replicated in vitro ; however, multi-species microbial and microcosms approaches have been proposed for the development of artificial caries lesions. 7 , 9 , 10 In this context, despite these more complex biofilm models producing artificial lesions similar to the natural ones, differences in protocols can impact the outcomes of studies evaluating lesion progression or arrest.…”