Evolution of mirror-image pain in temporomandibular joint osteoarthritis mouse model

Rotpenpian, Nattapon; Tapechum, Sompol; Vattarakorn, Anchalee; Chindasri, Wongsathit; Care, Chit; Pakaprot, Narawut; Wanasuntronwong, Aree

doi:10.1590/1678-7757-2020-0575

Cited by 11 publications

(20 citation statements)

References 40 publications

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“…This study was approved by the Animal Care and Use Committee, Faculty of Medicine, Siriraj Hospital, Mahidol University (SI-ACUP014/2561) and was performed according to the Animal Research: Reporting In Vivo Experiments (ARRIVE) Guidelines. The sample size was calculated to provide 80% power (1-β) with a 95% confidence interval (α = 0.05) [14]. Thirty adult male ICR mice (6 weeks old, 28-32 g initial body weight) were randomly divided into 5 groups of 6: a Sham treatment group and 4 CFA groups also receiving vehicle, ibuprofen, or one of 2 ECa 233 doses (30 and 100 mg/kg).…”

Section: Study Groups and Experimental Designmentioning

confidence: 99%

“…To induce a condition resembling TMJ-OA, mice were first injected intraperitoneally with sodium pentobarbital (60 mg/kg) for anesthesia and then with 10 µl of pure normal saline (NSS) (Sham group) or NSS containing 50% CFA (1 mg/ml) (F5881; Sigma-Aldrich, St. Louis, MO, USA) into the right TMJ. Injections were conducted once at the beginning of the experiment according to the previous studies [14,17,18]. The anatomical landmark for injection was also described in a previous study [17].…”

Section: Development Of the Tmj-oa Mouse Modelmentioning

confidence: 99%

“…The needle was then moved slowly until it passed under the edge of the arch and entered the TMJ space. After the needle reached the TMJ space, 10 µl of NSS with or without 50% CFA was injected slowly over a period of 5 s under control of a Hamilton syringe [14,17]. The body weight of each mouse was monitored throughout the 28-day experimental period to determine whether TMJ pain alters eating habits or general health.…”

Section: Development Of the Tmj-oa Mouse Modelmentioning

confidence: 99%

“…We therefore speculated that a TMJ-OA model could be established by local exposure to an exogenous proinflammatory agent such as complete Freund's adjuvant (CFA). In fact, previous studies have demonstrated that CFA injection into the TMJ of mice causes inflammation and induces behavioral signs of chronic TMJ-OA-like pain in response to non-noxious stimuli [14,15] possibly by inducing sustained activation of primary pain afferents. Chronic activation of these afferents can result in peripheral sensitization and ultimately in central pain sensitization (allodynia and hyperalgesia) [16].…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of a standardized Centella asiatica (ECa 233) extract against allodynia in a mouse model of temporomandibular osteoarthritis

Rotpenpian¹,

Wanasuntronwong²,

Tapechum³

et al. 2022

ScienceAsia

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Prolonged inflammation causing tissue injury can induce chronic pain hypersensitivity (allodynia and hyperalgesia). Osteoarthritis of the temporomandibular joint (TMJ-OA) is a common cause of chronic allodynia encountered in dental practice, but many currently available treatments induce intolerable side effects. In this study, we investigated the potential efficacy of a standardized Centella asiatica extract (ECa 233) on allodynia in a TMJ-OA mouse model established by an injection of complete Freund's adjuvant (CFA) into the TMJ. After CFA injection, animals daily received oral administration of vehicle, 0.14 g/kg ibuprofen (positive control), 30 mg/kg ECa 233, or 100 mg/kg ECa 233. Behavioral pain responses were examined by air-puff tests before and after CFA injection on days 3, 7, 14, 21, and 28. On day 28, TMJ-OA pathology was assessed by changes in articular cartilage thickness and graded according to the Osteoarthritis Cartilage Histopathology Assessment (OCHA) system. In the CFA + vehicle group, pain response scores increased gradually, reaching statistical significance compared to untreated Sham controls on days 14, 21, and 28. On day 28, OCHA grade score was 3.5 ± 0.35, and articular cartilage thickness was reduced compared to the Sham group. Both ECa 233 doses significantly attenuated pain response scores and also slowed degeneration of the TMJ with efficacy comparable to ibuprofen. We conclude that ECa 233 can protect against mechanical allodynia and cartilage degeneration in a mouse model of TMJ-OA.

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Section: Study Groups and Experimental Designmentioning

confidence: 99%

Section: Development Of the Tmj-oa Mouse Modelmentioning

confidence: 99%

Section: Development Of the Tmj-oa Mouse Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Efficacy of a standardized Centella asiatica (ECa 233) extract against allodynia in a mouse model of temporomandibular osteoarthritis

Rotpenpian¹,

Wanasuntronwong²,

Tapechum³

et al. 2022

ScienceAsia

Self Cite

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show abstract

“…4 It can be triggered in response to light touch/pressure stimuli, which are normally perceived as innocuous. 5 Patients with chronic pain, such as complex regional pain syndrome (CRPS) and atypical facial pain, etc., can develop MIP to the unaffected body side. 6,7 Complex regional pain syndrome type-I (CRPS-I) is a chronic pain condition that usually impacts the extremities of the patients.…”

Section: Introductionmentioning

confidence: 99%

Gene Expression Profiling of Contralateral Dorsal Root Ganglia Associated with Mirror-Image Pain in a Rat Model of Complex Regional Pain Syndrome Type-I

Nie

Liu

Yin

et al. 2021

JPR

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Background: Mirror-image pain (MIP), which develops from the healthy body region contralateral to the actual injured site, is a mysterious pain phenomenon accompanying many chronic pain conditions, such as complex regional pain syndrome (CRPS). However, the pathogenesis of MIP still remains largely unknown. The purpose of this study is to perform an expression profiling to identify genes related to MIP in an animal model of CRPS-I. Methods: We established a rat chronic post-ischemic pain (CPIP) model to mimic human CRPS-I. RNA-sequencing (RNA-Seq), bioinformatics, qPCR, immunostaining, and animal behavioral assays were used to screen potential genes in the contralateral dorsal root ganglia (DRG) that may be involved in MIP. Results: The CPIP model rats developed robust and persistent MIP in contralateral hind paws. Bilateral DRG neurons did not exhibit obvious neuronal damage. RNA-Seq of contralateral DRG from CPIP model rats identified a total 527 differentially expressed genes (DEGs) vs sham rats. The expression changes of several representative DEGs were further verified by qPCR. Bioinformatics analysis indicated that the immune system process, innate immune response, and cell adhesion were among the mostly enriched biological processes, which are important processes involved in pain sensitization, neuroinflammation, and chronic pain. We further identified DEGs potentially involved in pain mechanisms or enriched in small-to medium-sized sensory neurons or TRPV1-lineage nociceptors. By comparing with published datasets summarizing genes enriched in pain mechanisms, we sorted out a core set of genes which might contribute to nociception and the pain mechanism in MIP. Conclusion:We provided by far the first study to profile gene expression changes and pathway analysis of contralateral DRG for the studying of MIP mechanisms. This work may provide novel insights into understanding the mysterious mechanisms underlying MIP.

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Nociceptive receptors are expressed differently in trigeminal nociception after lingual nerve injury and unilateral external carotid artery occlusion in rats

Wanasuntronwong

Punyawattananon

Rotpenpian

et al. 2021

Archives of Oral Biology

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Evolution of mirror-image pain in temporomandibular joint osteoarthritis mouse model

Cited by 11 publications

References 40 publications

Efficacy of a standardized Centella asiatica (ECa 233) extract against allodynia in a mouse model of temporomandibular osteoarthritis

Efficacy of a standardized Centella asiatica (ECa 233) extract against allodynia in a mouse model of temporomandibular osteoarthritis

Gene Expression Profiling of Contralateral Dorsal Root Ganglia Associated with Mirror-Image Pain in a Rat Model of Complex Regional Pain Syndrome Type-I

Nociceptive receptors are expressed differently in trigeminal nociception after lingual nerve injury and unilateral external carotid artery occlusion in rats

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