Effect of a calcium hydroxide-based intracanal medicament containing N-2-methyl pyrrolidone as a vehicle against Enterococcus faecalis biofilm

Kim, Taegun; Kim, Mi-Ah; Hwang, Yun-Chan; Rosa, Vinícius; Fabbro, Massimo Del; Min, Kyung-San

doi:10.1590/1678-7757-2019-0516

Cited by 8 publications

(8 citation statements)

References 20 publications

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“…A flexible and tapered plastic needle is attached to the syringe to allow the paste to easily flow into the space. In previous in vitro studies comparing the removal efficiency of Calcipex [ 25 , 26 ], additional instrumentation or agitation was not applied after injecting of Calcipex paste. We confirmed the filling quality of the intracanal medication using micro-CT.…”

Section: Discussionmentioning

confidence: 99%

Sonic irrigation for removal of calcium hydroxide in the apical root canal: A micro-CT and light-coupled tracking analysis

2022

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Objective This study aimed to evaluate the efficacy of three sonic irrigation systems for removal of calcium hydroxide dressing from the apical root canal. Materials and methods A total of 96 single-rooted teeth in three categories of root canal curvatures (straight: 0–5°, moderate: 6–20°, and severe: > 20°) were allocated to four groups: conventional needle irrigation, EndoActivator, EQ-S, and Vibringe. The root canals were instrumented using Protaper NEXT and filled with calcium hydroxide. After removal of calcium hydroxide, the remaining volume of calcium hydroxide was measured by micro-CT analysis. Data were compared among root canal curvatures and irrigation systems using the Kruskal-Wallis test and Mann-Whitney test (p < .05). The oscillating range of each irrigation system was measured using light-coupled motion tracking. Results The volumes of calcium hydroxide remaining in the canals with severe curvature were significantly higher than in those of straight curvature (p < .05). In the canals of moderate or severe curvature, EQ-S showed the highest removal percentage, followed by EndoActivator, Vibringe, and needle irrigation (p < .05). Light-coupled tracking showed the largest oscillating range in EQ-S (p < .05). Conclusions Sonically activated irrigation systems with a flexible tip can be beneficial for calcium hydroxide intracanal dressing removal in the curved apical canals.

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Section: Discussionmentioning

confidence: 99%

Sonic irrigation for removal of calcium hydroxide in the apical root canal: A micro-CT and light-coupled tracking analysis

2022

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“…Furthermore, bovine root canals have wide surfaces; therefore, a standardized sample can be easily obtained. Therefore, bovine teeth have been widely used as specimens for endodontic microbiological studies [ 19 , 21 , 38 , 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

Combined Effect of Melittin and DNase on Enterococcus faecalis Biofilms and Its Susceptibility to Sodium Hypochlorite

et al. 2020

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Biofilm communities are tolerant to antimicrobials and difficult to eradicate. This study aimed to investigate the effect of melittin, an antimicrobial peptide, either alone or in combination with deoxyribonuclease (DNase), an inhibitor of extracellular deoxyribonucleic acid (eDNA), against Enterococcus faecalis (E. faecalis) biofilms, and biofilm susceptibility to sodium hypochlorite (NaOCl). Biofilms of E. faecalis were developed in root canals of bovine teeth. The biofilms were treated with distilled water (control), melittin, DNase, or DNase+melittin. The antibiofilm effects of the treatments were analyzed using colony forming unit (CFU) assay, crystal violet staining, confocal laser scanning microscopy (CLSM), and field emission scanning electron microscope (FE-SEM). The susceptibility of DNase+melittin-treated biofilms to NaOCl (0%, 2.5% and 5%) was investigated by the CFU assay. The data were statistically analyzed using one-way analysis of variance, followed by Tukey’s test. A p-value of <0.05 was considered significant. Specimens treated with DNase+melittin showed a more significant decrease in the CFUs, eDNA level, and biofilm formation rate than those treated only with melittin or DNase (p < 0.05). CLSM analysis showed DNase+melittin treatment significantly reduced the volume of biofilms and extracellular polymeric substance compared to either treatment alone (p < 0.05). FE-SEM images showed a high degree of biofilm disruption in specimens that received DNase+melittin. 2.5% NaOCl in specimens pretreated with DNase+melittin showed higher antibacterial activity than those treated only with 5% NaOCl (p < 0.05). This study highlighted that DNase improved the antibiofilm effects of melittin. Moreover, DNase+melittin treatment increased the susceptibility of biofilms to NaOCl. Thus, the complex could be a clinical strategy for safer use of NaOCl by reducing the concentration.

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“…The solvent NMP, along with the presence of calcium ions, appears to play a crucial role in this reactivity. Solutions of calcium ions in N-methyl-2-pyrrolidine have shown unusual reactivity in many areas, including the synthesis of thermally stable polyamides (Mallakpour & Kolahdoozan, 2008;Faghihi, 2009;Faghihi et al, 2010;Dewilde et al, 2016), the synthesis and structural studies of functional coordination polymers from calcium carboxylates based on cluster-and rod-like building blocks (Kang et al, 2014), dental applications using calcium hydroxide paste along with NMP (Lim et al, 2017;Kim et al, 2020), the formulation of solid self-nanoemulsifying drug-delivery systems (Agrawal et al, 2015), and in lyotropic liquid crystalline behavior of poly(2-cyano-p-phenylene terephthalamide) in N-methyl-2-pyrrolidone/calcium chloride solutions (Jung et al, 2016).…”

Section: Chemical Contextmentioning

confidence: 99%

Synthesis, structure, and theoretical studies of a calcium complex of a unique dianion derived from 1-methylpyrrolidin-2-one

Butcher¹,

Purdy²,

Brown³

et al. 2021

Acta Cryst E

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The title compound, catena-poly[[tetrakis(1-methylpyrrolidin-2-one-κO)calcium(II)]-μ-(E)-1,1′-dimethyl-2,2′-dioxo-1,1′,2,2′-tetrahydro-[3,3′-bipyrrolylidene]-5,5′-bis(thiolato)-κ2 O:O′], [Ca(C10H8N2O2S2)(C5H9NO)4] n , 1, crystallizes in the triclinic space group P\overline{1}. The crystal studied was twinned by non-merohedry via two different twofold operations, about the normals to (001) and (1\overline{1}0), giving four twin domains with refined occupancies of 0.412 (4), 0.366 (4), 0.055 (1), 0.167 (4). The Ca atoms are located on centers of inversion. Each Ca is surrounded by four 1-methylpyrrolidin-2-one (NMP) ligands and coordinated through one of the two O atoms to two (E)-1,1′-dimethyl-2,2′-dioxo-1,1′,2,2′-tetrahydro-[3,3′-bipyrrolylidene]-5,5′-bis(thiolate), [C10H8N2O2S2]2−, dianions (abbreviation: DMTBT). This dianion thus facilitates the formation of a 1-D polymer, which propagates in the [011] direction. These ribbons are linked by intermolecular C—H...S interactions. Each Ca atom is in an octahedral CaO6 six-coordinate environment with Ca—O bond lengths ranging from 2.308 (6) to 2.341 (6) Å, cis bond angles ranging from 88.2 (2) to 91.8 (2)° and the trans angles all 180° due to the Ca atoms being located on centers of inversion. Theoretical calculations were carried out using density functional theory (DFT) and the results showed that although the central DMTBT dianion is planar there is likely some resonance across the central bond between both azapentyl rings, but this is not sufficient to establish a ring current. The calculated UV–vis spectrum shows a peak at 625 nm, which accounts for the deep blue–purple color of solutions of the complex.

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Effect of a calcium hydroxide-based intracanal medicament containing N-2-methyl pyrrolidone as a vehicle against Enterococcus faecalis biofilm

Cited by 8 publications

References 20 publications

Sonic irrigation for removal of calcium hydroxide in the apical root canal: A micro-CT and light-coupled tracking analysis

Sonic irrigation for removal of calcium hydroxide in the apical root canal: A micro-CT and light-coupled tracking analysis

Combined Effect of Melittin and DNase on Enterococcus faecalis Biofilms and Its Susceptibility to Sodium Hypochlorite

Synthesis, structure, and theoretical studies of a calcium complex of a unique dianion derived from 1-methylpyrrolidin-2-one

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