Effect of enhanced masticatory force on OPG, RANKL and MGF in alveolar bone of ovariectomized rats

Abstract

Menopause induces oral bone loss, leading to various oral diseases. Mastication importantly affects bone metabolism in the jawbone. Objective: To analyze the effect of enhanced masticatory force on osteoprotegerin (OPG), receptor activator of nuclear factor kappa B ligand (RANKL), and mechanogrowth factor (MGF) in alveolar bone of ovariectomized rats and to study the mechanics mechanism of the alveolar bone of ovariectomized rats response to enhanced masticatory force. Methodology: Thirty Sprague Dawley rats were randomly divided into three groups: sham-operation group (fat around the removed ovary + normal hard diet), model group (ovariectomy + normal hard diet), and experimental group (ovariectomy + high hard diet). It was a 2-month experiment. Enzyme-linked immunosorbent assay (ELISA) detected serum estradiol (E2), osteocalcin (BGP) and alkaline phosphatase (ALP) in rats. Bone histomorphometric indices in the third molar region of maxilla were detected by micro-CT; protein expressions of OPG, RANKL, and MGF in the third molar region of maxilla was detected by Western blot; and gene expression of OPG, RANKL, and MGF in the third molar region of maxilla was detected by Quantitative Real-Time PCR. Results: Comparing with model group, serum E2 in experimental group increased but not significantly, serum BGP and serum ALP in experimental group decreased but not significantly, OPG in experimental group in alveolar bone increased significantly, RANKL in experimental group in alveolar bone decreased significantly, RANKL/OPG ratio in experimental group decreased significantly, MGF in experimental group in alveolar bone increased significantly, bone volume to total volume fraction increased significantly in experimental group, trabecular thickness increased significantly in experimental group, and trabecular separation decreased significantly in experimental group. Conclusion: Enhanced masticatory force affected the expression of OPG, RANKL, and MGF in alveolar bone of ovariectomized rats, improved the quality of jaw bone of ovariectomized rats, and delayed oral bone loss by ovariectomy.

The anti-osteoporotic effect of Moringa oleifera leaves extract on glucocorticoids-induced jawbone osteoporosis in Albino rats

“…The RT-PCR analysis for the RANKL gene expression showed a significant decrease, while the OPG gene expression showed a remarkable increase in the MO treated group compared to osteoporotic group. In addition, gene expression levels of RANKL and OPG in MO treated group were close to those of healthy rats as stated by Meijie et al and Ma et al [33,34]. Adhikary et al had supporting results where dietary flavonoid kaempferol inhibited glucocorticoid induced bone loss.…”

Total Flavonoids of Rhizoma Drynariae Ameliorate Bone Growth in Experimentally Induced Tibial Dyschondroplasia in Chickens via Regulation of OPG/RANKL Axis

“…It is then transferred into the nucleus as transcriptional enhancers by activating runt-related transcription factor-2 (Runx2) and promoting osteoblast differentiation and maturation ( Nuschke et al, 2014 ; Pierrefite-Carle et al, 2015 ). In recent years, osteoprotegerin (OPG)/receptor activator of nuclear factor kappa-B ligand (RANKL) axis has been extensively studied that the high expression of OPG and low expression of RANKL can effectively promote the expression of osteoblasts and accelerate the recovery of bone in through ( Ibáñez, et al, 2019 ; Amin, et al, 2020 ; Ma, et al, 2020 ), which may be through the combination of OPG and the key factor RANKL in the process of osteoclast differentiation to inhibit the combination of RANKL and RANK, thereby blocking the differentiation of osteoclast precursors into osteoclasts ( Udagawa et al, 2021 ). However, the underlying mechanism of OPG/RANKL axis to regulate tibial development in TD broilers remains largely unknown.…”

High-Throughput Metabolomics Discovers Metabolic Biomarkers and Pathways to Evaluating the Efficacy and Exploring Potential Mechanisms of Osthole Against Osteoporosis Based on UPLC/Q-TOF-MS Coupled With Multivariate Data Analysis

“…The oviduct was ligated by a silk thread, and the other side is operated in the same way. The muscle and skin layers were sutured with medical biological thread, and then disinfected with iodophor (Kim et al, 2019;Maiz et al, 2019;Zongmin et al, 2020). Rats in the SHA group were performed a sham surger under the same protocol.…”