Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate

Abstract

Purpose To compare, both qualitatively and quantitatively, the inflammatory cells, vascular density and IL-6 immunolabeled cells present in the pulp after pulpotomy with white MTA versus 15.5% ferric sulfate (FS). Methodology Forty-eight mandibular first molars from 24 Wistar rats were divided into MTA or FS groups and subdivided according to the period after pulpotomy procedure (24, 48 and 72 hours). Four teeth (sound and untreated) were used as controls. Histological sections were obtained and assessed through the descriptive analysis of morphological aspects of pulp tissue and the quantification of inflammatory cells, vascular density and interleukin-6 (IL-6) expression. Data were statistically analyzed (p<0.05). Results The number of inflammatory cells was similar in both groups, being predominantly localized at the cervical radicular third. In the MTA group, increased inflammation was observed at 48 hours. Vascular density was similar in both groups and over time, being predominant in the medium radicular third. No correlation was found between the number of inflammatory cells and the vascular density. Pulp tissue was more organized in MTA-treated teeth. In both groups, a weak to moderate IL-6 expression was detected in odontoblasts and inflammatory cells. Comparing both groups, there was a greater IL-6 expression in the cervical radicular third of teeth treated with MTA at 24 hours and in the medium and apical thirds at 72 hours, while in the FS group a greater IL-6 expression was found in the apical third at 24 hours. Conclusion The MTA group presented better histological features and greater IL-6 expression than the FS group. However, no difference was observed between the groups regarding the inflammatory status and vascularization, suggesting the usefulness of FS as a low-cost alternative to MTA.

Dental pulp inflammatory/immune response to a chitosan-enriched fibrin hydrogel in the pulpotomised rat incisor

“…This suggests that most of the inflammatory response observed was related to the mechanical preparation of the tooth and not to the nature of the implanted material. Finally, this upregulation of IL-6 mRNA was comparable to the weak to moderate IL-6 expression localised at the cervical radicular third at 48 h detected in odontoblasts and inflammatory cells after pulpotomy of rat molars with MTA (Lopes et al, 2019).…”

Pulp Tissue Response to Platelets Rich Plasma, Platelets Rich Fibrin and Mineral Trioxide Aggregate as Pulp Capping Materials

“…However, PRF treated teeth exhibit the least count after 7 days. This could be related to the biocompatibility and bioactivity of PRF (12) . MTA had moderate inflammation reaction compared to the other treated material at one week, this moderate inflammation could be due to high alkalinity and this inflammation is a reversible process (13) .…”

Identification of key module and hub genes in pulpitis using weighted gene co-expression network analysis

“…Pulp-capping agents ought to harbor antibacteria, nontoxicity, antiinflammation and excellent sealing abilities, and ought to induce dentin mineralization [ 40 ]. The pulp capping using Mineral Trioxide Aggregate (MTA) could reduce inflammation cells, decrease vascular density, and regulate IL-6 expression in mandibular first molars from Wistar rats [ 41 ]. iRoot BP Plus has shown similar properties and result with MTA in the pulpectomy in dog teeth [ 42 ] and displays a superior clinical handling property compared with MTA.…”