Root canal dressings for revascularization influence in vitro mineralization of apical papilla cells

Abstract

Endodontic revascularization is based on cell recruitment into the necrotic root canal of immature teeth after chemical disinfection. The clinical outcome depends on the ability of surviving cells from the apical tissue to differentiate and promote hard tissue deposition inside the dentinal walls. Objective To investigate the effect of calcium hydroxide (CH) and modified triple antibiotic paste (mTAP – ciprofloxacin, metronidazole and cefaclor) on the viability and mineralization potential of apical papilla cells (APC) in vitro . Material and Methods APC cultures were kept in contact with CH or mTAP (250-1000 µg/mL) for 5 days, after which cell viability was assessed using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Next, APCs were subjected to CH or mTAP at 250 µg/mL for 5 days before inducing the differentiation assay. After 14 and 21 days, calcium deposition was assessed by the Alizarin Red S staining method, followed by elution and quantification using spectrophotometry. Data were analyzed using ANOVA followed by Tukey post hoc test. Results CH induced cell proliferation, whereas mTAP showed significant cytotoxicity at all concentrations tested. APC treated with CH demonstrated improved mineralization capacity at 14 days, while, for mTAP, significant reduction on the mineralization rate was observed for both experimental periods (14 and 21 days). Conclusion Our findings showed that CH induces cell proliferation and improves early mineralization, whereas mTAP was found cytotoxic and reduced the mineralization potential in vitro of APCs.

Effectiveness of Autologous Platelet Concentrates in Management of Young Immature Necrotic Permanent Teeth—A Systematic Review and Meta-Analysis

“…Moreover, EDTA liberates the growth factors present in dentin that positively affect stem cell adhesion, migration, and differentiation [ 49 ]. TAP, especially in high concentrations, has cytotoxic effects on stem cells and reduces mineralization [ 50 ], and, when minocycline is included, can cause significant tooth discoloration [ 51 ]. Heterogeneity was also present in the protocol to obtain the autologous platelet concentrates.…”

Clinical Outcome and Comparison of Regenerative and Apexification Intervention in Young Immature Necrotic Teeth—A Systematic Review and Meta-Analysis

“…The most preferred ICM used in RET is a triple antibiotic paste containing minocycline, ciprofloxacin and metronidazole, followed by calcium hydroxide paste [63]. AAE recommends 0.1 mg of TAP, but at high concentrations, it has a cytotoxic effect on stem cells and reduces mineralization [64] and when minocycline is included, it can cause significant tooth discoloration [65]. Overall, it can be concluded that RET is a successful intervention for the management of immature necrotic permanent teeth with high to moderate certainty.…”

Hidróxido de Cálcio na modulação de células da papila apical e ligamento periodontal in vitro

“…As citocinas, como o TNF-α, estimulam a osteoclastogênese de forma independente, enquanto outras citocinas promovem a expressão do RANKL, que leva à diferenciação de osteoclastos e a exercer sua função (Graves et al, 2011). Buck et al (2001) O Ca(OH)2 tem a capacidade de aumentar a taxa de mineralização in vitro (Rahhal et al, 2019). O aumento na mineralização celular por Ca(OH)2 pode ser devido à modulação da liberação do fator de crescimento (Jaunberzins et al, 2000) e em células da polpa dentária in vitro, os materiais contendo cálcio aumentaram a produção de TGF-β1 (Rashid et.…”