Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model

Abstract

Objective In this study, we aimed to histologically and immunologically evaluate the effect of diode laser treatment when applied adjunctive to scaling and root planing (SRP) in an experimental periodontitis model.Materials and methods We used Wistar-Albino rats (n=60) with average weight of 230 g. Experimental periodontitis was induced by ligature at the right and left first mandibular molar teeth in all rats. After 11 days, the ligature was removed and rats were divided into two groups. The control group (n=30) received only SRP treatment, while the laser group (n=30) received a diode laser (GaAlAs, 810 nm, 1 W, 10 J, 20 s) treatment adjunctive to SRP. Ten rats in each group were sacrificed after 7, 15, and 30 days. Histopathological examination was performed in the left mandible of rats. Myeloperoxidase (MPO) was evaluated by western blot in the gingival specimens from the right mandible.Results MPO levels in the laser group were statistically significantly lower compared with the control group (p≤0.05). There was no statistically significance at any time between MPO levels in the control group (p>0.05). MPO levels in the laser group at the 7th day were statistically significantly higher compared to the 15th (p≤0.05) and the 30th day (p≤0.05). Inflammatory cell infiltration decreased over time in both groups and was statistically significantly lower in the laser group than in the control group at all times (p≤0.01).Conclusions Within the limits of this study, we suggest that diode laser application is an adjunctive treatment because it reduced inflammation and MPO when applied in addition to SRP. On the other hand, more studies are needed for the assessment of the effects of diode laser application to periodontal tissues.

Evaluation of long‐term effects of diode laser application in periodontal treatment of poorly controlled type 2 diabetic patients with chronic periodontitis

“…Our results showed that there was no statistically significant difference in PI and CAL, and laboratory findings at 3 and 6 months between the SRP+DL group and the SRP group, but there was a greater reduction in GI and BoP in regions where deep periodontal pockets are located at both T1 and T2 in the SRP+DL group. These findings are consistent with the results of previous studies that reported that DL application could reduce local inflammation and bone resorption markers, increase bone formation markers and stimulate collagen formation . The fact that there were statistically significant differences especially in deep periodontal pockets supports that DL application may increase the healing potential of the periodontal tissues in areas where conventional methods are inadequate for treatment.…”

The Effect of Four Weeks of Low-Level Laser Radiation (660 nm) on Movement Recovery and Fibroblasts Invasion

“…In this study, the radiation of low-level laser (660 nm) started 30 minutes after the induction of injury to prevent the expansion of the injury as much as possible before the beginning of destructive events. Likewise, the improvement of motion observed from the first week of the treatment in this study can be the evidence to the fact that the use of LLL, a well-known therapeutic method to control inflammatory, can be a good alternative in the first line of treatment after SCI (13,(22)(23)(24)(25). LLL reduces the secretion of cytokines and inflammatory factors, such as TNF-α, IL-1β, and IL-6 that were increased due to the inflammation (26).…”

“…As mentioned before, SCI complications are divided into two parts of primary and secondary phases that occur shortly after the injury to the spinal cord and lead to apoptosis and necrosis. The secretion and activation of preinflammatory factors such as IL1, tumor necrosis factoralpha (TNF-α) I, and IL-6 begin to increase the minutes after trauma and following it the equilibrium between the production of ROS and antioxidants becomes unbalanced, and oxidative stress happens that lead to cell death (13). In this regard, the increase of ROS is reported around inflammation (13,21).…”

“…The secretion and activation of preinflammatory factors such as IL1, tumor necrosis factoralpha (TNF-α) I, and IL-6 begin to increase the minutes after trauma and following it the equilibrium between the production of ROS and antioxidants becomes unbalanced, and oxidative stress happens that lead to cell death (13). In this regard, the increase of ROS is reported around inflammation (13,21). Apoptosis begins about an hour after the spinal cord injury in the center of the injury, and expands and continues until two weeks later on both rostral and caudal sides of the spinal cord (22).…”

“…Low-level laser therapy is one of the anti-inflammatory treatments that its usage to treat disease associated with inflammation has increased in recent years (12)(13)(14). Although there is not any complete protocol provided on this topic yet, the conducted researches have indicated that low-level laser is effective in the reduction of post-SCI complications (15)(16)(17).…”