Influence of Helicobacter pylori culture supernatant on the ecological balance of a dual-species oral biofilm

Zhang, Wenling; Deng, Xin; Zhou, Xuedong; Hao, Yuqing; Li, Yuqing

doi:10.1590/1678-7757-2017-0113

Cited by 13 publications

(10 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, most severe dental caries were observed in rats that had been infected with both S. mutans and H. pylori. A recent in vitro study revealed that H. pylori could change the balance of oral biofilm induced by oral streptococci 27 ; the authors of that study suggested that H. pylori may affect signalling among oral streptococci involved in biofilm formation, and that H. pylori could create an advantageous environment for S. mutans. Indeed, higher numbers of S. mutans were isolated from excised mandibular bones of rats infected with both S. mutans and H. pylori, compared with mandibular bone from rats infected with S. mutans alone.…”

Section: Discussionmentioning

confidence: 99%

“…A recent study showed that H. pylori was unsuitable for growth assays involving co-cultivation with oral streptococci, due to the stringent growth requirements of H. pylori 27 . In that study, the H. pylori culture supernatant was added to broth cultures of oral streptococci, which allowed analysis of the effect of H. pylori on the growth of oral streptococci.…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial growth of H. pylori was assessed using a previously described method 27 , with some modifications. H. pylori colonies were suspended in brucella broth supplemented with horse serum, then grown to an OD 550 of 0.2.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Contribution of Streptococcus mutans to Helicobacter pylori colonisation in oral cavity and gastric tissue

Nomura

Kadota

Ogaya

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Helicobacter pylori is presumed to infect gastric tissue via the oral cavity in childhood, whereas risk factors for H. pylori infection in the oral cavity are unknown. In this study, we analysed the effects of Streptococcus mutans, a major cariogenic bacterial species, on H. pylori colonisation in the oral cavity, as well as gastric tissue. Rats in the weaning period were infected with S. mutans in the oral cavity, then fed a caries-inducing diet to facilitate S. mutans colonisation. One month after S. mutans infection, rats were infected with H. pylori in the oral cavity; rats were then euthanised at 1 month after H. pylori infection. H. pylori was detected in the oral cavities of rats infected with both S. mutans and H. pylori, but not in rats infected with H. pylori alone. In addition, H. pylori colonisation in the gastric tissue and typical gastrointestinal damage were observed in rats infected with both S. mutans and H. pylori. When H. pylori was co-cultured with in vitro biofilm formed by S. mutans, a large number of H. pylori bacteria invaded the biofilm formed by S. mutans. Our results suggest that S. mutans is involved in the establishment of H. pylori infection. Helicobacter pylori, a helix-shaped gram-negative microaerophilic bacterium, is a major causative agent of gastric cancer and gastric ulcers 1. More than half of the world's population is infected with H. pylori 2 , which is presumably acquired mainly via the oral cavity in childhood 3,4. Molecular biological techniques have reportedly revealed H. pylori in oral specimens 5-7. The presence of H. pylori in the oral cavity has been related to the detection of H. pylori in the gastric tissue 8. However, details regarding risk factors of H. pylori infection in the oral cavity have not been clarified, which may explain the current difficulty in elimination of H. pylori infection. Streptococcus mutans, a gram-positive facultative anaerobe, is a major causative pathogen of dental caries 9. S. mutans is acquired in the oral cavity during early childhood, mainly via mother-to-child transmission 10. The aetiology of dental caries caused by S. mutans was clarified in the early 1960s 11 ; S. mutans metabolises sucrose to form a biofilm on the tooth surface, followed by demineralisation of the tooth. Nevertheless, eradication of S. mutans from the oral cavity and dental caries remains difficult 12. Some epidemiological studies have revealed that patients with dental caries or poor oral hygiene were more likely to harbour H. pylori in oral cavity or gastric tissue 13,14. These findings suggest that the presence of cariogenic bacteria is involved in infection of the oral cavity with H. pylori. To the best of our knowledge, no clear evidence has been obtained regarding the effects of S. mutans on H. pylori infection in an animal model. In the present study, we hypothesised that S. mutans colonisation in the oral cavity may be involved in H. pylori colonisation in both oral cavity and gastric tissue. Therefore, we constructed a rat co-infection model with S. mut...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Contribution of Streptococcus mutans to Helicobacter pylori colonisation in oral cavity and gastric tissue

Nomura

Kadota

Ogaya

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…After filtering, the residual liquid was used for extraction again. The content of soluble sugars was measured with an anthrone‐sulfuric acid method at 630 nm (Zhang et al 2018).…”

Section: Methodsmentioning

confidence: 99%

Phosphate availability regulates flavonoid accumulation associated with photosynthetic carbon partitioning in Cyclocarya paliurus

Zhang

Zhao

et al. 2021

Physiologia Plantarum

View full text Add to dashboard Cite

Cyclocarya paliurus has traditionally been used as medicine and functional food. This study aims at investigating the flavonoid accumulation in C. paliurus dependent on phosphate (Pi) availability and its potential association with internal carbon partitioning. One‐year‐old seedlings of C. paliurus were planted in four different Pi levels. Low Pi resulted in low phosphorus content within plants, while the nitrogen content increased. Further analysis revealed that the surplus carbon pool was greater and was allocated to N‐metabolism and carbohydrate synthesis under low Pi conditions, as shown by the higher levels of free amino acids, starch, and soluble sugars. Low Pi availability also induced higher enzymatic activities of shikimate dehydrogenase (SDH) and flavonoid 3‐hydoxylase (FHT), and higher flavonoid accumulation in leaves. Our results indicated that the surplus carbon induced by low‐Pi levels can increase flavonoid synthesis in seedlings of C. paliurus. In addition, growth and biomass accumulation were increased by the elevated Pi levels. As a result, the highest flavonoid yield per plant was obtained under relative low Pi conditions. This study can provide the basis for developing new agricultural practices to maintain high yield while still keeping the quality of medicinal plants and crops.

show abstract

“…It was shown that the surface topography and roughness have a great influence on the attachment of bacteria to a material surface. Moreover, the attached bacteria and the nucleating biofilm may be inhibited by both surface nanotopography and surface chemistry [ 122 , 123 , 124 ]. Figure 2 shows the general approach and the advantage of polymers with peculiar modifications such as addition of chemical cues that have antibacterial activity.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Nanocomposites Based on Biodegradable Polymers

et al. 2018

View full text Add to dashboard Cite

In the present review paper, our main results on nanocomposites based on biodegradable polymers (on a time scale from 2010 to 2018) are reported. We mainly focused our attention on commercial biodegradable polymers, which we mixed with different nanofillers and/or additives with the final aim of developing new materials with tunable specific properties. A wide list of nanofillers have been considered according to their shape, properties, and functionalization routes, and the results have been discussed looking at their roles on the basis of different adopted processing routes (solvent-based or melt-mixing processes). Two main application fields of nanocomposite based on biodegradable polymers have been considered: the specific interaction with stem cells in the regenerative medicine applications or as antimicrobial materials and the active role of selected nanofillers in food packaging applications have been critically revised, with the main aim of providing an overview of the authors’ contribution to the state of the art in the field of biodegradable polymeric nanocomposites.

show abstract

Influence of Helicobacter pylori culture supernatant on the ecological balance of a dual-species oral biofilm

Cited by 13 publications

References 29 publications

Contribution of Streptococcus mutans to Helicobacter pylori colonisation in oral cavity and gastric tissue

Contribution of Streptococcus mutans to Helicobacter pylori colonisation in oral cavity and gastric tissue

Phosphate availability regulates flavonoid accumulation associated with photosynthetic carbon partitioning in Cyclocarya paliurus

Nanocomposites Based on Biodegradable Polymers

Contact Info

Product

Resources

About