Determination of the effective anticandidal concentration of denture cleanser tablets on some denture base resins

Hayran, Yeliz; Sarıkaya, Işıl; Aydın, Ali; Tekin, Yadel Hazır

doi:10.1590/1678-7757-2017-0077

Cited by 35 publications

(17 citation statements)

References 27 publications

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“…The results of the present study was in agreement with recent studies which reported that, thermoplastic denture base material has lesser biofilm development and candidal count as compared to heat cure acrylic resin (58,59) . They explained this might be caused by the porosity of heat-cured acrylic resin, which easily triggers the piling of food debris and microorganisms inside (59) .…”

Section: Discussionsupporting

confidence: 93%

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An In-Vitro Evaluation of Alternative Disinfection Methods of Acrylic resin and Thermoplastic Resin Denture Base Materials

Kholief

Kabeel

2018

ADJ-for Girls

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Objectives: This study was designed to evaluate the antimicrobial effect of alternative disinfection methods of heat cured acrylic resin and thermoplastic resin denture base materials and to investigate the porosity resulting from disinfection by these methods. Material and Methods: Disc specimens of heat cured acrylic resin and thermoplastic resin were fabricated and divided into four groups for each denture base material according to the disinfection method. The specimens were contaminated in vitro by standardized suspensions of Candida albicans (C. albicans).The following test agents were used: distilled water (as a control group), 5% sodium hypochlorite (NaOCl), 100% white vinegar and 650 W microwave (MW) energy. After the disinfection procedure, the number of viable microbial cells was counted in CFU/ml. Porosities of acrylic resin and thermoplastic resin specimens were evaluated with Scanning Electron Microscope (SEM) after one month of daily exposure to disinfection. Results: ANOVA test showed that, there was a statistical significant difference among the tested treatment agents against C. albicans in each denture base material(P≤ 0.05). Post Hoc test showed a statistical significant difference between the control samples and the other disinfected samples in the microbial count, however, there was no statistical significant difference among the tested methods of disinfection (P>0.05).Regarding porosity area percentage, there was a statistical significant difference among the tested methods of disinfection (P≤ 0.05), where, 100% white vinegar showed the highest porosities followed by NaOCl then the MW disinfection and the least is the control group. Regarding comparison between the two denture base materials, thermoplastic resin showed statistically significant lower microbial adherence as well as lower porosity area percentage than heat cured acrylic resin(P≤ 0.05). Conclusion: All the tested disinfection methods shown to be efficient against C. albicans. Thermoplastic resin demonstrated lower microbial adherence than heat cured acrylic resin and lower porosity area percentages.

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Section: Discussionsupporting

confidence: 93%

“…The surface irregularities on denture base materials may act as a reservoir of infection and increase the possibility of hosting microorganisms even after the cleaning of dentures. Rough and porous surfaces make easier the penetration of bacterial and fungal cells on the denture base resins (36,58,59,63) .…”

Section: Discussionmentioning

confidence: 99%

An In-Vitro Evaluation of Alternative Disinfection Methods of Acrylic resin and Thermoplastic Resin Denture Base Materials

Kholief

Kabeel

2018

ADJ-for Girls

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“…Polidents are available as commercial products, which can easily provide enough cleansing without causing damage to denture resins. 14 According to the manufacturer’s instructions, 5 min of soaking or overnight soaking can kill most microbes. In the present study, we found that the solution of 20 µg/mL AgBr-NP@CTMAB showed similar antifungal activity to inhibit biofilm formation as Polident.…”

Section: Discussionmentioning

confidence: 99%

“…However, this sanitary method is not sufficient to completely eliminate microbial biofilms. 14 The program of denture cleaning has always been an interesting field to researchers, but there is no ideal method to meet the clinical cleaning requirements.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA

Zhang

Liu

Zhang

et al. 2021

IJN

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Objective To synthesize and determine the antifungal activity of AgBr-nanoparticles (NP) @CTMAB (cetyltrimethyl-ammonium bromide) against Candida albicans ( C. albicans ) for use in the field of denture cleaning. Methods The morphology and structure of AgBr-NP@CTMAB were characterized by IR, UV-Vis, XRD and SEM. The antifungal potential of AgBr-NP@CTMAB against C. albicans was determined by colony formation assay and growth curve analysis. PMMA containing AgBr-NP@CTMAB was prepared, and the long-term antifungal efficacy was analyzed. The effect against C. albicans biofilm was analyzed by SEM and OD600 , and the color changes of the specimens were observed by stereomicroscopy after 1 week of incubation. Cytotoxicity to human oral gingival fibroblasts and oral mucosal epithelial cells was detected by Cell Counting Kit-8 (CCK-8) in vitro. Results The compound showed a good crystalline phase, the presence of AgBr nanoparticles and the hybridization of CTMAB+ with AgBr-NPs. AgBr-NP@CTMAB showed significant antifungal activity against C. albicans at concentrations of 10 μg/mL and 20 μg/mL. PMMA specimens containing AgBr-NP@CTMAB showed no long-term antifungal effect against C. albicans biofilm. The clearance rate of C. albicans attached to PMMA was 44.73% after soaking in 10 µg/mL AgBr-NP@CTMAB solution for 30 min and 91.35% for 8 h. There was no significant residual cytotoxicity or visual color change after soaking. Significance AgBr-NP@CTMAB showed promising potential treatment for denture cleaners.

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“…[1][2][3][4] In addition, the majority of complete denture users have advanced age, physical and mental disabilities, systemic diseases, immunosuppression, and impaired manual dexterity for a proper hygiene, [1][2][3] thus increasing the risk of local problems, such as prosthetic stomatitis, 5,6 and systemic infections due to inhalation and ingestion of microorganisms that detach from the mucosa and the denture base. 7 The methods recommended for biofilm removal from the denture can damage the acrylic resin. 8 A suggested alternative was the addition of biocidal agents to the acrylic resin, such as silver nanoparticles, quaternary ammonium, nanosilicon dioxide, nanotitanium dioxide, 2-tert-butylaminoethyl methacrylate.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and Elemental Release of Dental Acrylic Resin Modified with Silver and Vanadium Based Antimicrobial Nanomaterial

Castro¹,

Teixeira²,

Alves³

et al. 2021

J. Health Scie.

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The acrylic resin used for the prosthesis base accumulates biofilm, causing diseases such as stomatitis. The addition of some nanoparticles promotes antimicrobial action. This study incorporated the nanostructured silver vanadate decorated with silver nanoparticles (AgVO3) to the acrylic resin by two methods and evaluated the cytotoxicity for human gingival fibroblasts (HGF) and the released silver and vanadium ions. The concentrations of 0.5, 1, 2.5, and 5% of AgVO3 was incorporated by vacuum spatulation and polymeric film. The vacuum spatulation was performed for 60 s using the Turbomix equipment, and the polymeric film was obtained from the polymer solubilization in chloroform, the film was subjected to a cryogenic grinding, and the powder obtained was manually mixed at the monomer. HGF cell viability was assessed after 24 hours, 7 and 14 days by the MTT assay. The release of silver (Ag) and vanadium (V) ions were quantified by inductively coupled plasma mass spectrometry after 30 days. Kruskal-Wallis and Dunn’s test were applied (α = 0.05). The HGF viability was inversely proportional to the incubation time. Both incorporation techniques and the negative and positive control groups presented significant statistical differences (p<0.05). The experimental groups presented no statistical difference compared to the negative control (p>0.05), except the vacuum spatulation group with 5% of AgVO3 that showed greater viability than the negative control (p=0.013) in 24 hours. The release of Ag and V ions was proportional to the concentration of AgVO3 The 5% group presented a significant difference compared to the other groups (p<0.05). In conclusion, the acrylic resin with and without the AgVO3 incorporation had a small cytotoxic potential for HGF in 24 hours, with a lower viability in longer contact times; the release of Ag and V ions was proportional to the concentration of AgVO3, not influencing cell viability. Keywords: Acrylic Resins. Cell Survival. Nanotechnology. Ions. ResumoA resina acrílica utilizada para a base da prótese acumula biofilme, causando doenças como a estomatite. A adição de algumas nanopartículas promove ação antimicrobiana. Este estudo incorporou o vanadato de prata nanoestruturado decorado com nanopartículas de prata (AgVO3) à resina acrílica por dois métodos e avaliou a citotoxicidade para fibroblastos gengivais humanos (HGF) e os íons prata e vanádio liberados. As concentrações de 0,5%, 1%, 2,5% e 5% de AgVO3 foram incorporadas por espatulação a vácuo e filme polimérico. A espatulação a vácuo foi realizada por 60 s no equipamento Turbomix, e o filme polimérico foi obtido a partir da solubilização do polímero em clorofórmio, o filme foi submetido a uma moagem criogênica e o pó obtido foi misturado manualmente ao monômero. A viabilidade celular de HGF foi avaliada após 24 horas, 7 e 14 dias pelo ensaio de MTT. A liberação de íons prata (Ag) e vanádio (V) foi quantificada por espectrometria de massa com plasma indutivamente acoplado após 30 dias. Os testes de Kruskal-Wallis e Dunn foram aplicados (α=0,05). A viabilidade de HGF foi inversamente proporcional ao tempo de incubação. As técnicas de incorporação e os grupos controle negativo e positivo apresentaram diferença estatisticamente significante (p<0,05). Os grupos experimentais não apresentaram diferença estatística em relação ao controle negativo (p>0,05), exceto o grupo de espatulação a vácuo com 5% de AgVO3 que apresentou maior viabilidade que o controle negativo (p = 0,013) em 24 horas. A liberação de íons Ag e V foi proporcional à concentração de AgVO3. O grupo 5% apresentou diferença significativa em relação aos demais grupos (p <0,05). Em conclusão, a resina acrílica com e sem a incorporação de AgVO3 apresentou um pequeno potencial citotóxico para o HGF em 24 horas, com menor viabilidade nos tempos de maior contato, e a liberação de íons Ag e V foi proporcional à concentração de AgVO3, não influenciando na viabilidade celular. Palavras-chave: Resinas Acrílicas. Sobrevivência Celular. Nanotecnologia. Íons.

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Determination of the effective anticandidal concentration of denture cleanser tablets on some denture base resins

Cited by 35 publications

References 27 publications

An In-Vitro Evaluation of Alternative Disinfection Methods of Acrylic resin and Thermoplastic Resin Denture Base Materials

An In-Vitro Evaluation of Alternative Disinfection Methods of Acrylic resin and Thermoplastic Resin Denture Base Materials

Synthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA

Cytotoxicity and Elemental Release of Dental Acrylic Resin Modified with Silver and Vanadium Based Antimicrobial Nanomaterial

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