Identification of Candida species isolated from cows suffering mastitis in four Brazilian states

Sartori, Luciana; Santos, R. C. dos; Marin, José Moacir

doi:10.1590/1678-7576

Cited by 14 publications

(15 citation statements)

References 9 publications

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“…In this study, Candida species of yeasts were isolated in the 23.44% (60/256) of all samples analyzed. This rate is a marginally higher than the rates reported in Turkey were 12.7% [ 9 ], 17.7% [ 10 ]; the rates reported in Brazil were 17.3% [ 4 ], 12.8% [ 11 ]; and the rate reported in Slovenia was 7.5% [ 12 ]. C. krusei was the predominant species isolated in this study, which was 23.33% (14/60) of the Candida isolates from those with clinical mastitis.…”

Section: Discussionmentioning

confidence: 72%

“…However, there is some discrepancy among rates that were also reported by others. Pengov et al reported a 34% [ 12 ], De&Marin et al reported a 44.5% [ 4 ], and Sartori et al reported a 34.6% [ 11 ] of C. krusei isolates from cows with mastitis. Their data reported a higher frequency of C. krusei infection than findings in this report.…”

Section: Discussionmentioning

confidence: 99%

“…In animals, C. parapsilosis are also of great importance in veterinary medicine because of their ability to infect many animal species, including cows [ 14 , 17 , 18 ]. Indeed, many studies have described the isolation of C. parapsilosis from milk samples with a frequency varied from 1.7% to up to 25.4%, depending on the sanitary condition and environmental factors [ 10 , 11 , 15 , 19 ].…”

Section: Discussionmentioning

confidence: 99%

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Epidemiological investigation of non-albicans Candida species recovered from mycotic mastitis of cows in Yinchuan, Ningxia of China

Wang

Luo

et al. 2018

BMC Vet Res

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BackgroundCandida spp. is the vital pathogen involved in mycotic mastitis of cows. However the epidemiology and infection of Candida species in mycotic mastitis of cow in Ningxia province of China has not been explored. In the present study, the epidemiology, antimicrobial susceptibility and virulence-related genes of non-albicans Candida (NAC) species were investigated.MethodsA total of 482 milk samples from cows with clinical mastitis in four herds of Yinchuan, Ningxia were collected and used for the isolation and identification of mastic pathogens by phenotypic and molecular characteristics, and matrix-assisted laser desorption ionization-time of flight mass spectrometry. The antimicrobial susceptibility to antifungal agents was also determined by a disk diffusion assay. The presence of virulence-related genes was determined by polymerase chain reaction (PCR).ResultsA total of 60 isolates from nine different Candida species were identified from 256 (60/256, 23.44%) milk samples. The most frequently identified species in cows with clinical mastitis groups were Candida krusei (n = 14) and Candida parapsilosis (n = 6). Others include Candida lipolytica, Candida lusitaniae, Cryptococcus neoformans. But no Candida albicans was identified in this study. Interestingly, All C. krusei isolates (14/14) were resistant to fluconazole, fluorocytosine, itraconazole and ketoconazole, 2 out of 14 C. krusei were resistant to amphotericin, and 8 out of the 14 were resistant to nystatin. Similarly, all six C. parapsilosis isolates were resistant to fluorocytosine, but susceptible to fluconazole, ketoconazole and nystatin; two of the six were resistant amphotericin and itraconazole. Molecularly, all of the C. parapsilosis isolates carried eight virulence-related genes, FKS1, FKS2, FKS3, SAP1, SAP2, CDR1, ERG11 and MDR1. All of the C. krusei isolates contained three virulence-related genes, ERG11, ABC2 and FKS1.ConclusionThese data suggested that Candida species other than C. albicans played a pathogenic role in mycotic mastitis of cows in Yinchuan, Ningxia of China. The high incidence of drug-resistant genes in C. parapsilosis and C. krusei also highlighted a great concern in public and animal health in this region.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Epidemiological investigation of non-albicans Candida species recovered from mycotic mastitis of cows in Yinchuan, Ningxia of China

Wang

Luo

et al. 2018

BMC Vet Res

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“…This organism may be an aetiological agent in cow mastitis (Dworecka-Kaszak et al 2012;Sartori et al 2014). The size and shape of these yeasts are similar to somatic cells and in order to distinguish them from SC we inoculated Candida albicans into distilled water and stained them with EtBr according to Method B.…”

Section: Discussionmentioning

confidence: 99%

Fluorescence microscopy methods for the determination of somatic cell count in raw cow's milk

Zajác¹,

Zubrická²,

Čapla³

et al. 2016

Vet. Med. - Czech

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ABSTRACT:The direct fluorescence microscopy method with ethidium bromide staining can be used for somatic cell counting in raw cow's milk. However, this method has some limitations that may influence the results of the analysis. We therefore aimed at improving the procedure of somatic cell nuclei staining. We tested the hypothesis that ethidium bromide can better penetrate into the DNA of cells with degraded somatic cell walls or into dead cells. Therefore, we increased the temperature of the sample to 100 °C in order to disrupt the somatic cell wall membrane and to improve ethidium bromide penetration to somatic cell nuclei. In all, 90 samples of raw cow's milk were analysed in this experiment. Three parallel measurements were performed using each of the microscopic methods and the routine flow cytometry method. In all, 810 microscopic smears were analysed. The somatic cells were counted using fluorescence microscopic methods and flow cytometry. The increased temperature during the sample preparation improved (P < 0.005) the penetration of ethidium bromide into the somatic cell nuclei. It is concluded that the direct fluorescence microscopy method is suitable for precise laboratory analysis of somatic cell in raw cow's milk.

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“…Bacterial opposition to beta-lactam mechanisms comprises of generation protein which is a low-affinity penicillin-binding as well as secretion of beta-lactamases (Ksouri et al 2015). Antibacterial agents from crops are substantial sources of new antibiotics compounds and efflux pump inhibitors which target bacterial virulence or can be adopted in mixture with other prevailing drugs (Sartori et al 2014). The plants generate a significant constituent of ethno-veterinary remedy that is implemented in the therapy of diverse disorders such as bovine mastitis (Sağlam et al 2017).…”

Section: Introductionmentioning

confidence: 99%

The New Mastitis Agents Emerged in Cattle in Turkey and An Investigation of Their Antimicrobial Susceptibility

Kenar

Aksoy

Köse

2019

Kocatepe Veterinary Journal

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There are new emerging cattle mastitis agents that have become a major burden economically on the dairy industry because of their negatively affects on production and quality in dairy cattle farming in Turkey,. To overcome this emerged problem, antimicrobials are adopted in this sector to prevent and administrate mastitis and other bacterial infections affecting cattle in the country. Nevertheless, the occurrence of antimicrobial resistance (AMR) is increasing in both animal and human contaminants. The occurrence and features of AMR of the emerging cattle mastitis agents in dairy cattle in Turkey, have been manifested. As a result, the goal of this research was to assess secluded emerging cattle mastitis agents in Turkey and to appraise the antimicrobial susceptibility of these pathogens. Sixty one milk samples from cattle wwith mastitis were collected between 2014 and 2018 for assessment of clinical mastitis in diagnostic and analysis laboratory of Faculty of Veterinary Medicine, Afyon Kocatepe University. Twenty five microorganism species arised as cattle mastitis agents were assessed in these milk samples. The outcomes of the present study identified the necessity for advancements in antimicrobial stewardship as well as infection administration plans in Turkish farms to decrease the occurence of AMR. VITEK Compact® 2 systemmethod showed that Streptococcus uberis had the maximum AMR while Globicatella sulfidifaciens had the minimum AMR. The study depicts that the number of cattle mastitis is directly proportional to the size of the herd.

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Identification of Candida species isolated from cows suffering mastitis in four Brazilian states

Abstract: Communication

Cited by 14 publications

References 9 publications

Epidemiological investigation of non-albicans Candida species recovered from mycotic mastitis of cows in Yinchuan, Ningxia of China

Epidemiological investigation of non-albicans Candida species recovered from mycotic mastitis of cows in Yinchuan, Ningxia of China

Fluorescence microscopy methods for the determination of somatic cell count in raw cow's milk

The New Mastitis Agents Emerged in Cattle in Turkey and An Investigation of Their Antimicrobial Susceptibility

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