Análise histomorfométrica do útero na espécie canina do nascimento aos seis meses de idade

Ramos, José Leonardo Gualberto; Ramos, Cristina Leite Francisco Gualberto; Cunha, Isabel Candia Nunes da; Carvalho, Eulógio Carlos Queiróz de; Shimoda, Eduardo; Luz, Marcelo Rezende

doi:10.1590/1678-7373

Cited by 2 publications

(2 citation statements)

References 14 publications

(10 reference statements)

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“…The female reproductive organs are complete at birth, except the uterus, which differentiates into three histological structures in the postnatal period: the endometrium, myometrium, and perimeter (Holst, 2019). The bitch's uterus develops continuously from birth to 180 days of life (Ramos et al, 2015), developing until the stage of the beginning of the estrous cycle. At each estrous cycle, the uterus prepares to harbor the conceptus through the action of ovarian hormones (Holst, 2019;Vermeirsch et al, 2000), which estrogen stimulates intense mitosis of endometrial cells in the proliferative phase, and progesterone stimulates hypertrophy and hyperplasia of them in the secretory phase (Aplin et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Histomorphometry and uterine proteomics during the normal reproductive cycle in bitches

Freitas

Vasconcelos

Moura

et al. 2021

RSD

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We aimed to evaluate the histomorphometry and proteomic profile of the canine uterus during all stages of the reproductive cycle. Eighteen healthy female dogs had their estrous cycle identified by clinical evaluation, vaginal cytology, and serum progesterone levels, which were allocated to the proestrus (n=5), estrus (n=5), diestrus (n=5), and anestrus (n=3) groups. All were submitted to elective ovariosalpingohysterectomy, and the uteri were collected for histomorphometric measurement (Image J software). For proteomic analysis, fragments of the uterine horns were subjected to protein measurement (Bradford method) and extraction by 2D electrophoresis (PDquest software). The results showed that the diestrus promoted greater values of thickness in the uterine structures (μm): uterine wall (2,223.8±229.8), endometrium (819.7±109.1), and myometrium (1,392.6±294.2). Uterus showed a protein profile with good reproducibility per phase (pI: 3.5–9.0; PM: 24–150 KDa), with 11 spots in all phases. Despite the greatest histomorphometric changes in the diestrus, we observed a greater number of spots in the estrus (253±45), followed by the proestrus (185±21), diestrus (113±39), and anestrus (80±21). This finding showed probable participation of these proteins in the uterine preparation for receiving gametes for fertilization. Our results showed greater uterine thickness in the diestrus, and greater protein secretion in the estrus, contributing to the prospection of identification of proteins responsible for the biological reproduction processes.

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Section: Introductionmentioning

confidence: 99%

Histomorphometry and uterine proteomics during the normal reproductive cycle in bitches

Freitas

Vasconcelos

Moura

et al. 2021

RSD

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“…In pigs, sheep, and cattle, the development of endometrial glands is a postnatal event, and can occur from birth until the 120 th day of life, depending on the species ( Gray et al ., 2001 ). In dogs, glandular development mainly occurs between birth and the 60th day of life ( Cooke et al ., 2012 ; Ramos et al ., 2015 ), however, a single postnatal supraphysiological dose of MPA (35 mg dog sc) was not able to ablate uterine adenogenesis ( Ponchon et al ., 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Partial ablation of endometrial glands in dogs after exposure to progestin during the neonatal period

et al. 2017

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Bitches with uteri devoid of endometrial glands should be sterile, and consequently could contribute to the population control of dogs. Considering that an inadequate exposure of the female reproductive system to steroids can lead to the formation of the uterine gland knock-out (UGKO) phenotype in some species, the aim of this study was to evaluate the effect of serial applications of medroxyprogesterone acetate (MPA) from birth until the age of six months on the development of endometrial glands in bitches. For this purpose, 16 female mongrel dogs from different litters were distributed into either an MPA group (n = 8), animals treated with 10 mg kg sc (Promone-E ® , Pfizer, Brasil) at 3-week intervals, from day one after birth until the age of six months, or a control group (n = 8), composed of animals that only received a 0.9% NaCl solution in place of MPA. At six months of age, ovariohysterectomy was performed and uterine horn samples were collected for histological and immunohistochemical examinations. The bitches from the MPA-treated group presented a 35% decrease in the number of endometrial glands, a larger diameter of the endometrial glands, a greater epithelial height, as well as a greater thickness of the uterine wall, endometrium, and myometrium. However, no significant differences were observed between the two groups in the expression of ER-α, ER-β, and PR on the surface epithelium and endometrial stroma. Therefore, the serial application of MPA from birth until the age of 6 months do not completely ablate the development of the endometrial glands in bitches, but impair it by 35%.

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Análise histomorfométrica do útero na espécie canina do nascimento aos seis meses de idade

Cited by 2 publications

References 14 publications

Histomorphometry and uterine proteomics during the normal reproductive cycle in bitches

Histomorphometry and uterine proteomics during the normal reproductive cycle in bitches

Partial ablation of endometrial glands in dogs after exposure to progestin during the neonatal period

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