Sanitizer resistance of biofilm-forming Salmonellaisolated from meat products

Milan, Camile; Agostinetto, A.; Conceição, R.C.S.; González, H.; Timm, Cláudio Dias

doi:10.1590/1678-7298

Cited by 7 publications

(11 citation statements)

References 6 publications

(2 reference statements)

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“…Estes resultados mostram que a maior população bacteriana que permaneceu após a ação dos sanitizantes sobre os isolados formadores de biofilme em relação ao não formador se deveu à maior população inicial encontrada nos biofilmes e não a diferenças de resistência bacteriana ou de acesso do sanitizantes às células bacterianas. Resultados semelhantes foram obtidos em outro trabalho [16], cujos autores chegaram a conclusões análogas às obtidas no nosso estudo, embora tenham testado os sanitizantes sobre biofilmes formados por isolados de Salmonella isolados de produtos cárneos.…”

Section: Discussionunclassified

“…Os isolados considerados formadores de biofilme nas placas de microtitulação foram testados quanto à capacidade de formar biofilme em diferentes superfícies, utilizando técnica já estabelecida [16], com pequenas modificações. Foram utilizadas placas de superfícies planas com 4 cm² de polietileno de alta densidade, aço inoxidável e vidro estéreis.…”

Section: Verificação Da Formação De Biofilme Em Diferentes Superfíciesunclassified

“…Utilizando o método previamente descrito [16], após a última lavagem, as placas com biofilme foram imersas em frascos contendo sanitizantes, onde permaneceram durante 10 min. Alcançado o tempo de contato estabelecido, as placas foram imersas em solução neutralizante (0,1 M Na 2 S 2 O 3 ) por 30 s. Após lavagem com PBS, foi passada uma zaragatoa estéril na superfície de cada placa e realizada contagem em ágar Baird-Parker.…”

Section: Sensibilidade Dos Isolados Em Biofilme a Sanitizantesunclassified

“…A ação de sanitizantes pode ser menos efetiva em bactérias formadoras de biofilmes, sendo incapaz de remover todos os microrganismos do biofilme, fato que pode levar a problemas de higiene e saúde pública, além de perdas econômicas [6,13,16].…”

Section: Introductionunclassified

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Biofilm Formation by Coagulase-Positive Staphylococcus aureus Isolated from Mozzarella Cheese Elaborated with Buffalo Milk and its Effect on Sensitivity to Sanitizers

Friedriczewski

Gandra

Conceição

et al. 2018

Acta Scientiae. Vet.

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Background:The buffalo milk mozzarella cheese is a new product in the market, with high consumer acceptance and excellent prospects for trade. The cheese is rich in nutrients, which favors the proliferation of microorganisms that can cause food-borne diseases in the consumer. Staphylococcus aureus can cause gastro-enteritis in humans by the production of enterotoxins in food. One problem that may hinder the elimination of undesirable microorganisms in the food industry is the formation of biofilms. The objective of this study was to determine the effect of biofilm formation by Staphylococcus aureus isolated from buffalo mozzarella cheese on sensitivity to sanitizers. Materials, Methods & Results: Fifty samples of buffalo mozzarella cheese were analyzed to investigate the presence of S. aureus. The isolates were obtained through microbiological analysis and identified by PCR. The similarity of the strains was compared through rep-PCR. The distinct strains were tested for biofilm formation in microtiter plates. Soy Tripticase Broth (TSB) was placed in each well of the microtiter plate and overnight cultures of each strain was added. Wells without bacterial culture were used as controls. A villous cap was then placed on the plate and incubated for 48 h at 37°C. During incubation, the biofilms formed on the surface of the villi of the caps. For quantification of biofilm formation, material that remained attached to the cap was stained with crystal violet, the stained biofilm was extracted and the OD570 of each well was measured. Each strain was classified as non-biofilm forming, weak forming, moderately formed or formative strong. Strong forming and non-biofilm forming strains were tested on high density polyethylene, stainless steel and glass surfaces. Plates of 4 cm² of the different materials were placed in TSB where the culture of each isolate was inoculated separately. At each 48 h incubation the plates were washed to remove unbound cells and re-inserted into TSB without the inoculum. After five replicates of the procedure, sterile swabs were passed over the entire surface of each plate for counting in Baird-Parker agar. They were also tested for sensitivity to sodium hypochlorite and iodine after biofilm formation. The biofilm plates were immersed in flasks containing sanitizers, where they remained for 10 min. At the established contact time, the plates were immersed in neutralizing solution for 30 s. After washing with PBS, a sterile swab was passed on the surface of each plate and counts on Baird-Parker agar were performed. The bands profiles obtained on rep-PCR were identical when compared to isolates from the same sample, indicating that each sample was contaminated with only one S. aureus strain. From the twenty S. aureus strain identified, two isolates were classified as strong biofilm formers, seven as moderate formers, ten weak formers and one as non-biofilm builder. The two strong forming strains produced biofilm on the three surfaces tested. The application of sodium hypochlorite and iodine...

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Section: Discussionunclassified

Section: Verificação Da Formação De Biofilme Em Diferentes Superfíciesunclassified

Section: Sensibilidade Dos Isolados Em Biofilme a Sanitizantesunclassified

Section: Introductionunclassified

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Biofilm Formation by Coagulase-Positive Staphylococcus aureus Isolated from Mozzarella Cheese Elaborated with Buffalo Milk and its Effect on Sensitivity to Sanitizers

Friedriczewski

Gandra

Conceição

et al. 2018

Acta Scientiae. Vet.

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“…A number of studies mentioned the ability of many bacteria to form biofilm on different surfaces. MILAN et al (2015) evaluated the biofilm-forming ability of two different isolates of Salmonella enterica subsp. enterica on high density polyethylene, stainless steel, and glass surfaces.…”

Section: Introductionmentioning

confidence: 99%

Biofilm formation by Vibrio parahaemolyticus on different surfaces and its resistance to sodium hypochlorite

Rosa

Conceição

et al. 2018

Cienc. Rural

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Vibrio parahaemolyticus is an important pathogen for both fish industry and consumers. It forms biofilm which makes it difficult to eliminate this microorganism using sanitizers. This study aimed to assess biofilm formation on different surfaces and effect of biofilm on resistance to sanitizers. Eight isolates of biofilm-forming V. parahaemolyticus were tested for the ability to form biofilms on a number of surfaces including high density polyethylene, stainless steel, glass, exoskeleton of Farfantepenaeus paulensis (Pink Shrimp), and operculum of Micropogonias furnieri (Whitemouth Croaker). Efficiency of sanitizer sodium hypochlorite against the bacteria was evaluated in the biofilms formed on the surface of the materials used; out the eight strains analyzed four formed biofilm on different surfaces. The present study shows that there are variations between surfaces in terms of biofilm formation, with more than one bacterial strain being able to form biofilm on the surface of the operculum of M. furnieri and on high density polyethylene as well. One isolate formed biofilm on glass, and one isolate formed biofilm on stainless steel. Sanitizers reduced biofilm formation on all surfaces. Based on our findings, we concluded that V. parahaemolyticus isolates have different ability to form biofilm on different surfaces. No isolates formed biofilm on shrimp shells. Results of this study also showed that sodium hypochlorite eat a concentration of 20 parts per million (20ppm) of Cl2, albeit not able to eliminate bacteria reported in biofilms, is still capable of reducing bacterial populations.

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Biosynthesis of gold nanoparticles and inhibition of various stages of bacterial biofilms formed by drug-resistant Aeromonas hydrophila, Escherichia coli, and Klebsiella pneumoniae

James

Kumar

2022

Arch Microbiol

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Sanitizer resistance of biofilm-forming Salmonellaisolated from meat products

Cited by 7 publications

References 6 publications

Biofilm Formation by Coagulase-Positive Staphylococcus aureus Isolated from Mozzarella Cheese Elaborated with Buffalo Milk and its Effect on Sensitivity to Sanitizers

Biofilm Formation by Coagulase-Positive Staphylococcus aureus Isolated from Mozzarella Cheese Elaborated with Buffalo Milk and its Effect on Sensitivity to Sanitizers

Biofilm formation by Vibrio parahaemolyticus on different surfaces and its resistance to sodium hypochlorite

Biosynthesis of gold nanoparticles and inhibition of various stages of bacterial biofilms formed by drug-resistant Aeromonas hydrophila, Escherichia coli, and Klebsiella pneumoniae

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