Classification of lymphoma in cats and its relationship with the detection of feline leukemia vírus proviral DNA

Abstract

In this retrospective and prospective study, histopathological and immunohistochemical analyses of 62 cases of lymphomas in cats were performed to classify the anatomic forms and subtypes, according to the WHO guidelines, and correlate it to FeLV proviral DNA detected using PCR. The most common anatomical form was gastrointestinal (40.3%, 25/62), followed by multicentric (29%, 18/62), mediastinal (17.7%, 11/62) and extranodal (12,9%, 8/62). Among the lymphoma subtypes, diffuse large B-cell lymphoma (DLBCL) (30.6%, 19/62) was the most commonly diagnosed followed by peripheral T-cell lymphoma (PTCL) (29%, 18/62) and enteropathy associated T-cell lymphoma type 2 (14.5%, 9/62). DNA extraction from paraffin-embedded neoplastic tissue was obtained in 28 cases and FeLV proviral DNA was detected by PCR, in 23 of these. Of the cases presenting with FeLV proviral DNA, nine (32%) were of the multicentric form, five (22%) of the mediastinal and extranodal forms and four (17%) of the gastrointestinal form. The most frequent subtypes with FeLV proviral DNA, independent of the anatomical form, were DLBCL (39.1%, 9/23) and PTCL (34.7%, 8/23). The presence of the FeLV proviral DNA in 23 cats of this study, probably had association with the multicentric form of lymphoma and higher occurrence in the DLBCL and PTCL subtypes.

Pathohistological features of mediastinal lymphoma in domestic cats

“…Cats are usually negative for the leukemia virus. In all other cases, 30-50% of animals retain a positive FeLV status (Little et al, 2007;Ota-Kuroki et al, 2014;Silva et al, 2022).…”

Aspectos Epidemilógicos, Clínicos E Terapêuticos De Felinos Com Linfoma E Positivos Para Leucemia Viral Felina (Felv) Atendidos Em Um Hospital Veterinário Universitário

“…O tratamento instituído para o câncer caracterizado como linfoma constitui-se de quimioterapia antineoplásica normalmente com associação de fármacos, os protocolos de eleição são: COP (ciclofosfamida, vincristina e prednisolona), LOPH (prednisolona, lomustina, vincristina e doxorrubicina) e CHOP (ciclofosfamida, vincristina, prednisolona, doxorrubicina e Laspariginase), sendo que a frequência das sessões irá depender do protocolo escolhido, sinais laboratoriais de agravamento da enfermidade e evolução clínica do paciente, porém normalmente se mantém sessões semanais que podem ter duração de meses ou anos (Silva, 2022).…”