ELISA of amyloid A in paired bronchoalveolar lavage fluid and serum samples of healthy horses

Abstract

Pulmonary disorders are common in horses, and treatment efficiency depends on an adequate diagnosis. Amyloid A is the most sensitive indicator of pathology in horses. The objective of this study was to establish the concentration of amyloid A of bronchoalveolar lavage fluid (BALF) in healthy horses. Health condition of horses was considered normal based on physical examination, complete blood count, biochemical parameters, and BALF cytology. Blood and BALF were collected from thirty adult female horses. Amyloid A concentrations in serum and BALF were measured using commercial ELISA tests. Amyloid A was detected in serum (mean ± SD = 3.71±2.51) and BALF (mean ± SD = 0.000745±0.000785) of all horses. In conclusion, SAA can also be measured in bronchoalveolar fluid, affording early detection of respiratory infections or inflammatory conditions.

Coleta e avaliação das secreções de vias aéreas posteriores em equinos: Revisão

“…Estudos recentes estão buscando descrever a detecção da AAS no fluido de lavagem broncoalveolar mediante teste ELISA. Já a HP em equinos pode ser usada como marcador de hemólise durante o exercício (Di-Filippo et al, 2020).…”

“…O diagnóstico presuntivo de inflamações das vias aéreas inferiores em equinos depende da associação de histórico, exame clínico e exames complementares (Di-Filippo et al, 2020), portanto, as intervenções realizadas por veterinários devem estar sempre amparadas pela boa realização das técnicas.…”