Use of the ACP® and BTS extenders for cooling at 15°C white-lipped peccary (Tayassu pecari) semen

Barros, Celso Henrique Souza Costa; Machado, William Morais; Vieira, Renan Luiz Albuquerque; Allaman, Ivan Bezerra; Nogueira-Filho, Sérgio Luiz Gama; Bittencourt, Rodrigo Freitas; Snoeck, P.P.N.

doi:10.1590/1678-5150-pvb-6053

Cited by 2 publications

(6 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1 Os escores variaram entre 0 (ausência da expressão comportamental) a 2 (apresentação máxima da expressão comportamental). 2 Não houve diferença estatística significativa entre os protocolos depois de aplicada a correção de Bonferroni para comparações múltiplas (P < 0,003).…”

Section: Resultsunclassified

“…As diferenças em volume e concentração espermática podem estar relacionadas com as diferenças comportamentais das espécies. Na natureza, queixadas vivem em grupos de centenas de indivíduos, enquanto caititus vivem em grupos bem menores, com 5 a 15 indivíduos e no máximo 50 animais [3]. Em cativeiro, queixadas apresentam hierarquia de dominância linear, enquanto não se consegue organizar os caititus de forma hierárquica [4].…”

Section: Discussionunclassified

“…Adicionalmente, foi feita a higienização do prepúcio com solução fisiológica (NaCl 0,9%) e seco com papel toalha. Logo após, foi feita a limpeza do reto do animal por meio da remoção do conteúdo fecal, para permitir melhor introdução da sonda e contato com a mucosa retal, a qual foi lubrificada com carboximetilcelulose (Carbogel ® ) 3 . Depois de anestesiados, 5 min antes da coleta, foram administrados 5 UI de ocitocina (Ocitocina Forte UCB ® ) 4 por via intravenosa (IV).…”

Section: Protocolos Anestésicosunclassified

“…Existem relatos, contudo, sobre a baixa fertilidade de machos de queixadas que dificultaria sua reprodução em cativeiro [25]. Adicionalmente, devido à hierarquia linear, o macho dominante possui acesso prioritário às fêmeas receptivas [16], acarretando diminuição na variabilidade genética [2,13]. A inseminação artificial pode se tornar uma ferramenta para evitar a perda da diversidade genética em cativeiro [20][21][22].…”

Section: Introductionunclassified

“…A inseminação artificial pode se tornar uma ferramenta para evitar a perda da diversidade genética em cativeiro [20][21][22]. Estudos recentes comprovaram a viabilidade da conservação do sêmen de queixadas [2,3]. Ainda são necessárias informações sobre métodos seguros de anestesia e coleta de sêmen nesta espécie.…”

Section: Introductionunclassified

See 4 more Smart Citations

Chemical Restraining and Electroejaculation Protocols for Semen Collection in White-Lipped Peccary (Tayassu pecari)

Vieira

Barros

Costa

et al. 2021

Acta Scientiae. Vet.

Self Cite

View full text Add to dashboard Cite

Background: In order to reverse the White-lipped peccary decline, besides protecting its habitat and controlling hunting, it is necessary a captive breeding program. There are reports, however, on the low fertility of white-lipped peccary, making it difficult its reproduction in captivity, making artificial insemination one of the main tools to prevent the loss of genetic diversity of species kept in captivity. Information on safe methods of anesthesia and the collection of semen should be investigated. Therefore, we aimed to compare the effects of the anesthetic protocols acepromazine/ketamine and xylazine/ketamine, as well as electroejaculation protocols, for semen collection in white-lipped peccary (Tayassu pecari).Materials, Methods & Results: Twelve adult male white-lipped peccaries were submitted both to the xylazine/ketamine and acepromazine/ketamine anesthetic protocols. The anesthetic induction time and duration, the degree of muscle relaxation, the time for anesthetic recovery and the quality of the animals’ recovery were evaluated. Additionally, the quality of the sedation was evaluated based on the animal’s behavior. We also evaluated the effect of drugs on erectile functions as well as the efficiency of three electroejaculation protocols with increasing or fixed voltages (2 to 4 V; 5 to 12 V; 12 V). The acepromazine/ketamine combination promotes shorter induction time, duration and recovery from anesthesia than the xylazine/ketamine association. There were no differences, however, between the tested anesthetic protocols in relation to heart rate, respiratory rate and temperature. Ejaculate was obtained from only 2 animals when using the xylazine/ketamine protocol and adoption of stimuli between 5 and 12 V, with 10 stimuli at each voltage. In turn, ejaculate was obtained from four animals submitted to the acepromazine/ketamine protocol, three of them with the adoption of stimuli between 5 and 12 V and one with the adoption of fixed 12 V stimuli, with 45 stimuli at this single voltage.Discussion: The animals presented less deep anesthesia and, consequently, worse indicators of well-being during and after the collection procedures when submitted to the xylazine/ketamine protocol compared to the acepromazine/ketamine protocol. When submitted to the acepromazine/ketamine protocol, the animals allowed the observer to approach and handle them, facilitating handling and collection of semen, in addition to promoting better indicators of animal welfare. Also, with this aforementioned protocol, the animals showed better anesthetic return. For both anesthetic protocols, the protocol of increasing stimuli from 5 to 12 V, with 10 stimuli at each voltage, resulted in penile erection and in obtaining ejaculate in a greater number of animals in relation to the other electroejaculation protocols. In turn, the use of the growing protocol 2 V to 4 V did not even cause an erection in any of the 12 animals. From the ejaculates collected from the white-lipped peccary, volumes (0.2 to 1.0 mL) and average sperm concentration (379.1 x 106 sperm/mL) were comparatively higher than those from Pecari tajacu. The white-lipped peccary (Tayassu pecari) is considered an aggressive animal, and this characteristic can explain the relatively low success in obtaining ejaculates, as aggressiveness is directly related to stress, which is an antagonist of ejaculation. Thus, we proposed to test chemical restraint with the aid of a blowgun in future studies.

show abstract

Section: Resultsunclassified

Section: Discussionunclassified

Section: Protocolos Anestésicosunclassified

Section: Introductionunclassified