Suture and venous traction test analysis in dogs fixed in alcohol and preserved in saline solution

Pelógia, Marina Emanoella Seruti; Cerqueira, Eduardo S.F.; Silveira, Camila Pinho Balthazar da; Rolim, Glauco S.; Fechis, Alisson Diego Senna; Rocha, Thiago André Salvitti de Sá; Laus, José Luiz; Oliveira, Fabrício Singaretti de

doi:10.1590/1678-5150-pvb-5447

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…The mean values for maximum rupture force of the fresh or preserved pericardium in glycerine varied between 50N and 60N, significantly below the 130N required for fresh skin rupture or 110N to 177N for dogs kept for up to 4 months in ethyl alcohol tanks (Rocha et al., 2018). However, it was much more significant than 19.98N to cause a rupture in dogs' external jugular veins or the 21N to 26N necessary to rupture the dogs' jugular veins in ethyl alcohol tanks for up to 4 months (Pelogia et al., 2018). The values were also higher than the maximum rupture force in common carotid arteries of fresh dogs (25.77 ± 15.43N) or kept in ethyl alcohol tanks (16.44 ± 31.79N) (Cerqueira et al., 2017).…”

Section: Discussionmentioning

confidence: 99%

Biomechanics of the fresh and conserved bovine pericardium

Soares

Oliveira

Queiroz

et al. 2021

Anat Histol Embryol

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Summary The use of biological membranes in surgeries is a reality for years, and one of the most used is the bovine pericardium, so the purpose of this research is to describe the bovine pericardium's biomechanics by comparing two directions of a test, one parallel to the longitudinal heart axis and one perpendicular. 20 adult bovine pericardium were tested for the maximum rupture force and rupture elongation, collecting four samples of each pericardium direction. In phase 2, eight pericardia were conserved for 4 months in a 98% glycerine solution, and the solution in which they were submerged was microbiologically analysed monthly. The Mann–Whitney test was used; there was a very significant difference between the perpendicular and parallel groups (p = .0001). The T test showed no significant difference for the rupture elongation (p = .0938). In pericardium preserved in glycerine, the outliers were removed regarding the maximum rupture force, and a Boxcox transformation was performed (λ = 0.25). Outliers were removed for the rupture elongation, and Bartlett's test (p = .7836), and Cramer–Von Mises (p = .5033) were performed and then, the analysis of variance (p < .0001), followed by the Tukey test at 5%. In the microbiological analysis, there was no presence of microorganisms during conservation. The research has shown that the pericardium collection direction influences its resistance and it can be stored in glycerine for 4 months without losing biomechanical characteristics.

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Section: Discussionmentioning

confidence: 99%

Biomechanics of the fresh and conserved bovine pericardium

Soares

Oliveira

Queiroz

et al. 2021

Anat Histol Embryol

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A new model for thoracic radiology teaching and research in cadavers of embalmed dogs

Kihara,

Zero,

Queiroz

et al. 2024

Cienc. Rural

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Nowadays, alternative methods that do not use formaldehyde to preserve cadavers must be used due to this substance’s toxicity. Synthetic models are a training option for teaching, but cost makes their use unviable in most underdeveloped countries. The present study’s main objective was to develop a model for education and researching thorax radiology in cadavers of chemically prepared dogs. Megaesophagus, pleural effusion, pneumothorax, and bronchography, as well as pulmonary insufflation, were simulated in 32 dogs cadavers, which received 150 mL/kg of pure ethyl alcohol solution with 5% glycerin followed by injection of 120 mL/kg of a solution containing 20% sodium chloride, 1% sodium nitrite and 1% sodium nitrate; they were kept refrigerated between 2 to 6 °C, for 30, 60, 90 or 120 days (G30, G60, G90, G120). There was no contamination, putrid odor, or subcutaneous emphysema. The pulmonary insufflation was kept, and the color and the consistency were similar to a fresh corpse after 120 days of conservation. It was possible to perform radiographic procedures, and almost all affections could be greatly mimicked. Megaesophagus and bronchography were easily simulated. Pneumothorax was the most challenging condition to be reproduced, especially in cadavers with some liquid in the thorax. The alcoholic and curing salt solutions are an embalming alternative with low financial and environmental costs and proved to preserve corpses.

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Suture and venous traction test analysis in dogs fixed in alcohol and preserved in saline solution

Cited by 2 publications

References 8 publications

Biomechanics of the fresh and conserved bovine pericardium

Biomechanics of the fresh and conserved bovine pericardium

A new model for thoracic radiology teaching and research in cadavers of embalmed dogs

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