Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

Abstract

Use of acute-phase proteins (APPs) for assessment of health and disease in animals has increased greatly within the last decade. The objective was to determine the normal concentration of APPs in the serum and cerebrospinal fluid (CSF) of healthy cattle by polyacrylamide gel electrophoresis. Fifty crossbred animals (350±70kg of BW and 18±1.2 months of age), 25 heifers and 25 steers were used. CSF samples were collected from atlanto-occipital (AO) site and blood samples were obtained from the jugular vein. CSF and serum protein electrophoresis were performed by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Thirty-seven proteins with molecular weights ranging from 7 and 37kDa were identified in CSF of all animals. These eight were nominally identified with immunoglobulin A and G, celuloplasmin, transferrin, albumin, α1-antitripsin, acidic glycoprotein, and haptoglobin. All protein fractions in CSF did not differ between heifers and steers. In sera, 34 proteins with molecular weights between 7 and 244kDa were identified in heifers and steers. Similar proteins were nominally identified in the sera, but only the CSF presented α1-antitripsin. The serum values of acidic glycoprotein and immunoglobulin G were significantly higher in steers compared with heifers. In conclusion, measurement of CSF acute phase protein concentrations can be useful in diagnosing and monitoring the progression of bovine neurological diseases, perhaps even to guide therapeutic procedures. The CSF electrophoretic profile of healthy cattle does not change depending on gender.

Choroid Plexus Blood-CSF Barrier: Major Player in Brain Disease Modeling and Neuromedicine

“…This transcriptome alteration centers around the so-called acute-phase response to peripheral inflammation. CP is ideally situated, with the help of CSF which carries the secreted proteins 37 , to pass immunosignaling molecules into the ventricle-brain axis.…”

Abnormalities of Serum Protein Electrophoresis in Cattle and Comparison between Assays for Markers of Inflammation and Markers of Liver Alteration

“…This anomaly reported in humans, has not been previously revealed in ruminants. Only the CSF in cattle (the cerebrospinal fluid) presented α1-antitripsin (Fagliari et al 2007;Sampaio et al 2015;Di Filippo 2018).…”

Expression of Acute Phase Protein, Glial Fibrillary Acidic Protein, Epithelial Cadherin, and S100 Protein in Brain Tissues from Natural Cases of Ovine Encephalitic Listeriosis

“…Extensive production of hepatic plasma proteins is a common systemic response in several diseases, and these proteins are collectively known as acute-phase proteins (APPs) (Lee et al, 2015). In an earlier report, the estimation of APPs in serum and cerebrospinal fluid (CSF), particularly amyloid A and haptoglobin, has been used as a diagnostic tool in several neurological disorders (Filippo et al, 2018;Nirala et al, 2020). However, its efficacy in the diagnosis of listeriosis has not been confirmed.…”