Transcriptome sequencing and screening of genes related to glucose availability in Schizosaccharomyces pombe by RNA-seq analysis

Abstract

While calorie restriction is the most used experimental intervention to increase lifespan in numerous model organisms, increasing evidence suggests that excess glucose leads to decreased lifespan in various organisms. To fully understand the molecular basis of the pro-aging effect of glucose, it is still important to discover genetic interactions, gene expression patterns, and molecular responses depending on glucose availability. Here, we compared the gene expression profiles in Schizosaccharomyces pombe mid-log-phase cells grown in three different Synthetic Dextrose media with 3%, 5%, and 8% glucose, using the RNA sequencing method. Expression patterns of genes that function in carbohydrate metabolism were downregulated as expected, and these genes were downregulated in line with the increase in glucose content. Significant and consistent changes in the expression were observed such as genes that encoding retrotransposable elements, heat shock proteins, glutathione S-transferase, cell agglutination protein, and conserved fungal proteins. We group some genes that function together in the transcription process and mitotic regulation, which have recently been associated with glucose availability. Our results shed light on the relationship between excess glucose, diverse cellular processes, and aging.

Oyster Mushroom (Pleurotus Ostreatus) Ethanolic Extract Protects Yeast Cells From Hyper- And Hypo-Glycemic Stress

“…In addition, glucose regulates the expression of genes predominantly those that encode proteins of the glycolytic and respiratory pathways, stress response, utilization of glucose or alternative carbon sources, or those that influence cell growth (Yilmazer et al, 2021). Manipulating the glucose concentration in the diet or growth media has multiple implications (Tarhan & Çakır, 2021). Elevated glucose shortened lifespan in Caenorhabditis elegans through downregulation of AMPK, FOXO, and glyoxalase, while in Saccharomyces cerevisiae reduction of glucose levels from 2% to 0.1% induced transient cell cycle delay followed by cell adaptation to low-glucose and prolonged yeast lifespan up to 50% (Lee et al, 2009;Saitoh & Yanagida, 2014;Jiang et al, 2000).…”