MicroRNA-296-5p is differentially expressed in individuals with and without HIV-1 infection

Cárdenas-Bedoya, Jhonathan; Marquez-Pedroza, Jazmin; Morán-Moguel, María Cristina; Escoto-Delgadillo, Martha; Vázquez-Valls, Eduardo; González-Enríquez, Gracia Viviana; Pérez-Ríos, Alma Minerva; Torres-Mendoza, Blanca Miriam

doi:10.1590/1678-4685-gmb-2020-0017

Cited by 7 publications

(3 citation statements)

References 24 publications

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“…Recent evidences indicate that miRNA-296-5p regulates liver PIN1 and PLK1 genes that encode peptidylprolyl Cis/Trans isomerase NIMA-Interacting-1 and polo-like kinase 1 respectively, both involved in HIV-1 replication cycle stages (18,19), suggesting that miRNA-296-5p may have a regulatory activity on virus replication in HIV pathogenesis and contributing to immune activation. These indicators could be potential biomarkers for disease progression and, in agreement with previous observations (48), good targets for therapies aimed at modulating the immune response in HIV infection. All explain herein, reinforce the idea that those distinct miRNAs could be considered regulators of cholesterol metabolism, hepatic lipoprotein synthesis, potential liver damage biomarkers and therapeutic targets for the treatment of CVD.…”

Section: Discussionsupporting

confidence: 88%

miRNA Profile Based on ART Delay in Vertically Infected HIV-1 Youths Is Associated With Inflammatory Biomarkers and Activation and Maturation Immune Levels

et al. 2022

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Early antiretroviral treatment (ART) in vertically acquired HIV-1-infection is associated with a rapid viral suppression, small HIV-1 reservoir, reduced morbimortality and preserved immune functions. We investigated the miRNA profile from vertically acquired HIV-1-infected young adults based on ART initiation delay and its association with the immune system activation. Using a microRNA panel and multiparametric flow cytometry, miRNome profile obtained from peripheral blood mononuclear cells and its association with adaptive and innate immune components were studied on vertically HIV-1-infected young adults who started ART early (EARLY, 0-53 weeks after birth) and later (LATE, 120-300 weeks). miR-1248 and miR-155-5p, were significantly upregulated in EARLY group compared with LATE group, while miR-501-3p, miR-548d-5p, miR-18a-3p and miR-296-5p were significantly downregulated in EARLY treated group of patients. Strong correlations were obtained between miRNAs levels and soluble biochemical biomarkers and immunological parameters including CD4 T-cell count and maturation by CD69 expression on CD4 T-cells and activation by HLA-DR on CD16high NK cell subsets for miR-1248 and miR-155-5p. In this preliminary study, a distinct miRNA signature discriminates early treated HIV-1-infected young adults. The role of those miRNAs target genes in the modulation of HIV-1 replication and latency may reveal new host signaling pathways that could be manipulated in antiviral strategies. Correlations between miRNAs levels and inflammatory and immunological markers highlight those miRNAs as potential biomarkers for immune inflammation and activation in HIV-1-infected young adults who initiated a late ART.

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Section: Discussionsupporting

confidence: 88%

miRNA Profile Based on ART Delay in Vertically Infected HIV-1 Youths Is Associated With Inflammatory Biomarkers and Activation and Maturation Immune Levels

et al. 2022

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“…Extracellular vesicles have roles in viral pathogenesis [8-10] and, much like retroviruses, shuttle molecules between cells, including microRNAs (miRNAs) [11,12] and small nuclear RNAs (snRNAs) [13][14][15]. We and others have reported correlations of miRNA expression with HIV infection, virus replication, and central nervous system (CNS) diseases using HIV-infected CD4 þ cell lines [16,17], total plasma [18][19][20][21], and extracellular vesicles [22][23][24][25][26]. However, our previous work identified relatively few robust miRNA differences in total plasma [18], and a preinfection time point was required to establish informative changes in abundance.…”

Section: Introductionmentioning

confidence: 99%

Longitudinal characterization of circulating extracellular vesicles and small RNA during simian immunodeficiency virus infection and antiretroviral therapy

Huang

Liao

Dang

et al. 2023

Aids

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Objectives:Latent infection by HIV hinders viral eradication despite effective antiretroviral treatment (ART). Among proposed contributors to viral latency are cellular small RNAs that have also been proposed to shuttle between cells in extracellular vesicles. Thus, we profiled extracellular vesicle small RNAs during different infection phases to understand the potential relationship between these extracellular vesicle associated small RNAs and viral infection.Design:A well characterized simian immunodeficiency virus (SIV)/macaque model of HIV was used to profile extracellular vesicle enriched blood plasma fractions harvested during preinfection, acute infection, latent infection/ART treatment, and rebound after ART interruption.Methods:Measurement of extracellular vesicle concentration, size distribution, and morphology was complemented with qPCR array for small RNA expression, followed by individual qPCR validations. Iodixanol density gradients were used to separate extracellular vesicle subtypes and virions.Results:Plasma extracellular vesicle particle counts correlated with viral load and peaked during acute infection. However, SIV gag RNA detection showed that virions did not fully explain this peak. Extracellular vesicle microRNAs miR-181a, miR-342–3p, and miR-29a decreased with SIV infection and remained downregulated in latency. Interestingly, small nuclear RNA U6 had a tight association with viral load peak.Conclusion:This study is the first to monitor how extracellular vesicle concentration and extracellular vesicle small RNA expression change dynamically in acute viral infection, latency, and rebound in a carefully controlled animal model. These changes may also reveal regulatory roles in retroviral infection and latency.

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“…EVs have roles in viral pathogenesis [8][9][10] and, much like retroviruses, shuttle molecules between cells, including microRNAs (miRNAs) 11,12 and small nuclear RNAs (snRNAs) [13][14][15] . We and others have reported correlations of miRNA expression with HIV infection, virus replication, and CNS diseases using HIV-infected CD4+ cell lines 16,17 , total plasma [18][19][20][21] , and EVs [22][23][24][25][26] . However, dynamic changes of EV attributes including miRNAs have not yet been fully characterized at different stages of retroviral infection and treatment.…”

Section: Introductionmentioning

confidence: 99%

Longitudinal characterization of circulating extracellular vesicles and small RNA during simian immunodeficiency virus infection and antiretroviral therapy

Huang

Liao

Dang

et al. 2022

Preprint

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Objectives: Latent infection by human immunodeficiency virus (HIV) hinders viral eradication despite effective antiretroviral treatment (ART), Amongst proposed contributors to viral latency are cellular small RNAs that have also been proposed to shuttle between cells in extracellular vesicles (EVs). Thus, we profiled EV small RNAs during different infection phases to understand the potential relationship between these EV-associated small RNAs and viral infection. Design: A well characterized simian immunodeficiency virus (SIV)/macaque model of HIV was used to profile EV-enriched blood plasma fractions harvested during pre-infection, acute infection, latent infection/ART treatment, and rebound after ART interruption. Methods: Measurement of EV concentration, size distribution, and morphology was complemented with qPCR array for small RNA expression, followed by individual qPCR validations. Iodixanol density gradients were used to separate EV subtypes and virions. Results: Plasma EV particle counts correlated with viral load and peaked during acute infection. However, SIV gag RNA detection showed that virions did not fully explain this peak. EV microRNAs miR-181a, miR-342-3p, and miR-29a decreased with SIV infection and remained downregulated in latency. Interestingly, small nuclear RNA U6 had a tight association with viral load peak. Conclusions: This study is the first to monitor how EV concentration and EV small RNA expression change dynamically in acute viral infection, latency, and rebound in a carefully controlled animal model. These changes may also reveal regulatory roles in retroviral infection and latency.

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MicroRNA-296-5p is differentially expressed in individuals with and without HIV-1 infection

Cited by 7 publications

References 24 publications

miRNA Profile Based on ART Delay in Vertically Infected HIV-1 Youths Is Associated With Inflammatory Biomarkers and Activation and Maturation Immune Levels

miRNA Profile Based on ART Delay in Vertically Infected HIV-1 Youths Is Associated With Inflammatory Biomarkers and Activation and Maturation Immune Levels

Longitudinal characterization of circulating extracellular vesicles and small RNA during simian immunodeficiency virus infection and antiretroviral therapy

Longitudinal characterization of circulating extracellular vesicles and small RNA during simian immunodeficiency virus infection and antiretroviral therapy

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