Use of DNA barcode in the identification of fish eggs in tributaries of the Paranapanema River basin

Abstract

Fish eggs are often excluded from identification analysis since at this stage of development there are few morphological characters. The correct identification of eggs can provide important information about spawning areas of species. The current work aimed to identify fish eggs in the Tibagi and Cinzas Rivers using the DNA barcode to obtain information on richness and diversity, adding to the existing data in the area. Of the 928 sequences analyzed using the BOLD Systems database, 99.78% were able to be identified at a specific level, demonstrating a high success rate for egg identification. The samples resulted in 25 species, 11 families, and 2 orders. Of the 25 species found, more than half (60%) present reproductive migration behavior, indicating that the tributaries of the Capivara reservoir are being used as a migratory route by these species. Eggs of rare and endangered species were found, indicating these tributaries as spawning grounds for these species. The results demonstrate the importance of identifying fish eggs in reservoir-influenced environments to recognize breeding areas of native and endangered species, as well as the importance of the Tibagi and Cinzas Rivers for the maintenance of native fish species in the Paranapanema River.

Broodstock development, final oocyte maturation and breeding of the newly described sparid fish, Fanged seabream, Sparidentex jamalensis Amir, Siddiqui & Masroor, 2014

“…The specimens were morphologically identical with the characteristics laid down for the species Sparidentex jamalensis as per Amir et al (2014). DNA barcoding (Hebert et al, 2003) is an efficient tool to distinguish cryptic fish species (Jacobina et al, 2020) at different stages of life, including eggs and larvae (Lima et al, 2020). S. jamalensis has been invariably confused with S. hasta till description (Amir et al, 2014) and the barcodes generated from this study confirmed the identity of the species as Sparidentex jamalensis .…”

Isolation and characterization of microsatellite loci in Pimelodus microstoma (Siluriformes: Pimelodidae)

“…Considering the five species of the family used for the transferability test, Pimelodus maculatus was the most successful in amplification, with eight out of ten polymorphic loci, success may correspond to the close phylogenetic relationship, being of the same genus that have a low genetic distance between species (D = 6.24 ± 1.08) (Lima et al., 2020). The locus Pmic04 was monomorphic for the main species of the study and for four others of the same family but was polymorphic when tested in the species Sorubim lima , showing three alleles in three individuals.…”