Association of long non-coding RNA MIAT and MALAT1 expression profiles in peripheral blood of coronary artery disease patients with previous cardiac events

Abstract

Long non-coding RNAs (lncRNAs) are implicated in various cellular and pathological processes. Two lncRNAs, myocardial infarction-associated transcript (MIAT) and metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), may be involved in the pathogenesis of coronary artery disease (CAD). Here, we aimed to determine the relative circulating levels of MIAT and MALAT1 in 110 stable CAD patients and 117 controls and to correlate their levels with the clinical and laboratory data. Peripheral blood expression levels were quantified by Real-Time qPCR. The median MIAT expression level in CAD patients was significantly 12-fold higher than controls (p<0.001). Otherwise, the median MALAT1 expression level was comparable in patient and control groups. Both lncRNAs showed significantly higher relative expression levels in patients with positive history of previous cardiac ischemic events, and MIAT showed significantly higher expression in diabetic CAD patients. The area under the curve of MIAT (0.888 ± 0.02 with sensitivity 95.5% and specificity 72.7%), was significantly larger than that of MALAT1 (0.601 ± 0.04 with sensitivity 50% and specificity 63.6%) for detecting the presence of significant CAD. The current findings suggest that lncRNA MIAT could have a diagnostic significance in CAD patients. MALAT1 levels, however, are not sufficiently reliable to have much clinical use in our cases.

Mechanism of long non‑coding RNA metastasis‑associated lung adenocarcinoma transcript 1 in lipid metabolism and inflammation in heart failure

“…Previous findings have shown that lncRNAs play an important role in regulating mammalian cardiogenesis (32). A study has revealed the association of MALAT1 expression profiles in peripheral blood of coronary artery disease patients with previous cardiac events (33). In this study, we demonstrated that lncRNA MALAT1 was highly expressed in HF patients and exhibited great value for HF diagnosis.…”

Circulating long non-coding RNAs NKILA, NEAT1, MALAT1, and MIAT expression and their association in type 2 diabetes mellitus

“…41 MIAT is particularly involved in diabetic retinopathy and numerous other microvascular problems. 42 A study by Toraih et al in 2019 43 revealed a significantly higher expression of circulating MIAT in patients with diabetes with coronary heart disease (CAD). Higher expression levels of MIAT and MALAT1 were related to hypertension and premature CAD, and both lncRNAs displayed greater relative expression in patients with a progressive history of prior cardiac ischaemic events.…”

lncRNA MIAT increases cell viability, migration, EMT and ECM production in age‑related cataracts by regulating the miR‑181a/CTGF/ERK signaling pathway

“…MIAT dysregulation has been identified in a number of different diseases. For example, previous studies investigating coronary artery disease indicated that MIAT expression was significantly elevated in the serum of patients with coronary artery disease ( 16 , 17 ). Furthermore, MIAT upregulation has been reported in ischemic stroke ( 18 ).…”

“…Moreover, certain lncRNAs have been reported to be associated with oxidative stress during cataract ( 13-15 ). LncRNA myocardial infarction associated transcript (MIAT) aberrant expression has been identified in a number of diseases, including coronary artery disease ( 16 , 17 ), ischemic stroke ( 18 ) and ARC ( 19 ). However, the biological mechanism underlying MIAT during ARC is not completely understood.…”