Identification of histone methylation modifiers and their expression patterns during somatic embryogenesis in Hevea brasiliensis

Abstract

Histone methylation plays a crucial role in various biological processes, from heterochromatin formation to transcriptional regulation. Currently, no information is available regarding histone methylation modifiers in the important rubber-producing plant Hevea brasiliensis. Here, we identified 47 histone methyltransferase (HMT) genes and 25 histone demethylase (HDM) genes as possible members of the histone methylation modifiers in the rubber tree genome. According to the structural features of HMT and HDM, the HbHMTs were classified into two groups (HbPRMs and HbSDGs), the HbHDMs have two groups (HbLSDs and HbJMJs). Expression patterns were analyzed in five different tissues and at different phases of somatic embryogenesis.

Differential Expression of lncRNAs and miRNAs Between Self-Rooting Juvenile and Donor Clones Unveils Novel Insight Into the Molecular Regulation of Rubber Biosynthesis in Hevea brasiliensis

“…The reactive oxygen species (ROS) production and ROS-scavenging in laticifers affect latex coagulation ( Zhang et al, 2019b ). In addition, transcription factors are actively implicated in the regulation of latex regeneration ( Wang et al, 2013b ; Li et al, 2016b , 2020a ; Zhang et al, 2019b ; Qu et al, 2020 ). In the present study, DELs acting in the cis-regulation were found.…”

“…XM_021836818.1 encoding DRM2 and XM_021803144.1 encoding histone deacetylase 9 are targeted by XR_002493724.1 and hbr-miR476. Histone deacetylase and DRM2 play essential roles in epigenetic modifications ( Pandey et al, 2002 ; Tian et al, 2005 ; Ashapkin et al, 2016 ; Zhang et al, 2018 ), and epigenetic modifications may lead to gene differential expression between self-rooting JCs and DCs ( Li et al, 2016a , 2017 , 2020a ), indicating that XR_002493724.1 has essential role in differential gene expression between SRJCs and DCs as hbr-miR476 target. However, the functions of these lncRNAs need further verification and study.…”

“…These genes and proteins might participate in natural rubber biosynthesis and reactive oxygen metabolism, which may provide remarkable functions in the high-yielding rubber in SRJCs ( Li et al, 2011a , b , 2014b , 2016a ). Notably, some findings showed that the differential gene expression between SRJCs and DCs related to the modification of DNA methylation, histone methylation, and acetylation ( Li et al, 2016a , 2017 , 2020a ).…”

Identification and Expression Analysis of Histone Modification Gene (HM) Family During Somatic Embryogenesis of Oil Palm

“…Somatic embryogenesis is an important tissue culture approach for plant regeneration and it incites various epigenetic changes such as histone modi cations (methylation, demethylation, acetylation, and deacetylation [24,25]. During the somatic embryogenesis process, various histone modi cations are potentially regulated [24,26]. The histone modi cations activity leads to the altered gene expression of somatic embryogenesis process [24].…”

“…During the somatic embryogenesis process, various histone modi cations are potentially regulated [24,26]. The histone modi cations activity leads to the altered gene expression of somatic embryogenesis process [24]. These histone modi cation changes regulated by various genes during somatic embryos potentially affect the development of somatic embryos.…”