Dealing with iron metabolism in rice: from breeding for stress tolerance to biofortification

Santos, Railson Schreinert dos; Júnior, Artur Teixeira de Araújo; Pegoraro, Camila; Oliveira, Antônio Costa de

doi:10.1590/1678-4685-gmb-2016-0036

Cited by 37 publications

(18 citation statements)

References 158 publications

(221 reference statements)

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“…Iron is a trace element is very important for rice plants for growth and development, especially for grain yield production through efficiency of photosynthesis by maintaining high chlorophyll production. However, when in excess, it becomes a highly toxic element [5,6]. Rice yield loss due to iron toxicity ranges from 10% to 100%, depending on the severity of the toxicity and the tolerance of rice varieties.…”

Section: Introductionmentioning

confidence: 99%

Effects of Iron on the Productivity of Lowland Rice (O. sativa L.) in Segregating Populations

Andrew¹,

Dorcas²,

Olawale³

2020

AJAF

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Rice plants have the tendency of taking up iron in the form of Fe 2+ , which is prevalent in paddy fields under flooded environments. But its deficiency or in excess of Fe 2+ in the soil affect several physiological functions of the plant. The objective of the study was to evaluates the effect of three ferrous sulphate concentration levels on the yield and yield components of lowland segregating rice populations. Three experiments were established in screenhouse concurrently in randomized complete block design in three replications in pots. Treatment comprised of 6 breeding lines each from two rice populations of F2 and F3 generations and two popular checks. Experiment one is the control without FeSO 4 treatment, while experiment two and three are F2 and F3 populations, respectively treated with FeSO 4 solution. Three concentration levels of FeSO 4 solution (600mg/kg of soil, 1200mg/kg of soil, and 1800mg/kg of soil,) were applied into each pots a week before transplanting in the treated experiments. Remarkable reduction in effective tiller number at 1800mg of Fe stress relative to the control was observed of 42.6% and 42.9% in F2 and F3 population, respectively. Significant reduction in grain yield of 33.5% and 36.4% at 1800mg of Fe compared to the control in F2 and F3 populations, respectively. The study showed that at 1200mg of Fe could be optimal for rice crop performance and at 1800mg of Fe becomes toxic to the plant as observed significant reduction in all agronomic traits especially in total grain yield. In F2 and F3 population, UPN 59, UPIA 2 and UPN 95 where the most stable genotypes across iron concentration levels. These genotypes could be used in population development for iron breeding programme.

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Section: Introductionmentioning

confidence: 99%

Effects of Iron on the Productivity of Lowland Rice (O. sativa L.) in Segregating Populations

Andrew¹,

Dorcas²,

Olawale³

2020

AJAF

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“…Efforts have been carried out, for example, in the development of transgenic plants, and different genes were assayed. Even though the strategies using genetic transformation resulted in the increase of Fe content, milling is still responsible for many losses (revised by Santos et al, 2017). Thus, the conventional selection of genotypes with high Fe content followed by hybridization continue to be an interesting alternative.…”

Section: Discussionmentioning

confidence: 99%

Iron biofortification in rice: in search of morphological traits for indirect selection in breeding programs

et al. 2019

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Iron (Fe) deficiency is an important cause of health concern in developing countries, demanding mitigation strategies to fight this disorder. The biofortification of staple foods, such as rice (Oryza sativa L.), could be achieved via the development of improved cultivars. Iron quantification is relatively costly and time-consuming, making its routine use impracticable in breeding programs. Therefore, the identification of traits to be used in indirect selection strategies would be of high interest. This study aimed to find promising traits for use in indirect selection programs aiming Fe biofortification in rice grains. A diverse set of 95 rice genotypes, mostly Brazilian, was grown at Southern Brazil, in 2016/2017, and Fe content in brown and polished grains, as well as other 12 morphological traits, were assayed. Analysis of variance, linear correlation, and path analysis were carried out. Different levels of association between traits were found, being panicles per plant and caryopsis width the most promising for use in indirect selection aiming increased Fe content in both brown and polished rice. However, secondary traits, such as caryopsis length, have also to be considered when performing selection aiming Fe biofortification in the cereal.

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“…These results suggest that transcriptional stability is dependent on tissue, genotype and the experimental conditions to which it has been subjected, and that the use of multiple reference genes is recommended for better normalization. Although studies of gene expression through RT-qPCR have been made with rice under stress by iron [ 13 ], this represents the first effort to investigate the transcriptional behavior of reference genes in rice under iron excess, an important condition of abiotic stress that is still neglected by studies in the field of molecular biology.…”

Section: Discussionmentioning

confidence: 99%

“…Among the most important abiotic stresses in rice is iron toxicity, a root related stress, that is usually associated with flooded soils and consequently with lowland production systems. In the absence of oxygen high concentrations of reduced iron (Fe 2+ ) become available in soil solution and the excessive uptake of this element can damage rice plants in several ways [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Selection and testing of reference genes for accurate RT-qPCR in rice seedlings under iron toxicity

et al. 2018

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Reverse Transcription quantitative PCR (RT-qPCR) is a technique for gene expression profiling with high sensibility and reproducibility. However, to obtain accurate results, it depends on data normalization by using endogenous reference genes whose expression is constitutive or invariable. Although the technique is widely used in plant stress analyzes, the stability of reference genes for iron toxicity in rice (Oryza sativa L.) has not been thoroughly investigated. Here, we tested a set of candidate reference genes for use in rice under this stressful condition. The test was performed using four distinct methods: NormFinder, BestKeeper, geNorm and the comparative ΔCt. To achieve reproducible and reliable results, Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines were followed. Valid reference genes were found for shoot (P2, OsGAPDH and OsNABP), root (OsEF-1a, P8 and OsGAPDH) and root+shoot (OsNABP, OsGAPDH and P8) enabling us to perform further reliable studies for iron toxicity in both indica and japonica subspecies. The importance of the study of other than the traditional endogenous genes for use as normalizers is also shown here.

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Dealing with iron metabolism in rice: from breeding for stress tolerance to biofortification

Cited by 37 publications

References 158 publications

Effects of Iron on the Productivity of Lowland Rice (O. sativa L.) in Segregating Populations

Effects of Iron on the Productivity of Lowland Rice (O. sativa L.) in Segregating Populations

Iron biofortification in rice: in search of morphological traits for indirect selection in breeding programs

Selection and testing of reference genes for accurate RT-qPCR in rice seedlings under iron toxicity

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