Genotoxic and mutagenic effects of polluted surface water in the midwestern region of Brazil using animal and plant bioassays

Abstract

This study aimed to evaluate DNA damage in animal and plant cells exposed to water from the Água Boa stream (Dourados, Mato Grosso do Sul, Brazil) by using bioassays, and to identify the chemical compounds in the water to determine the water quality in the area. Through the cytotoxicity bioassay with Allium cepa, using micronucleus test, and comet assay, using Astyanax altiparanae fish, the results indicated that biological samples were genetically altered. Micronuclei were observed in erythrocytes of A. altiparanae after exposure to water from locations close to industrial waste discharge. The highest DNA damage observed with the comet assay in fish occurred with the exposure to water from locations where the presence of metals (Cu, Pb, Cd, Ni) was high, indicating the possibility of genotoxic effects of these compounds. Thus, these results reinforce the importance of conducting genotoxicity tests for developing management plans to improve water quality, and indicate the need for waste management before domestic and industrial effluents are released into the rivers and streams.

Assessment of Cytological Effects of Food Preservative Potassium Metabisulphite to <i>Allium cepa</i>

“…A micronucleus may arise from a whole lagging chromosome or an acentric chromosome fragment detaching from a chromosome after breakage which does not integrate in the daughter nuclei [39]. Pollution of the environment with various chemical American Journal of Plant Sciences agents increases the frequency of micronucleus formation in both plants and animal cells, increasing the ecotoxicological risk [40] [41]. In accordance with Gömürgen (2005), such genetic damages like micronuleus may have clastogenic effect of their inducers [23].…”

Avaliação ambiental da qualidade limnologica e de sedimentos em córrego do Centro Oeste do Brasil

“…A cidade de Dourados é a maior entre aquelas que ocorrem total ou parcialmente dentro desta bacia, com aproximadamente 215 mil habitantes e a base da economia da região é a agricultura e a pecuária (Gonçalves et al, 2010;Paula et al, 2013). A bacia do rio Dourados é caracterizada nas suas proximidades por várias atividades agroindustriais, que acabam gerando contaminantes que são escoados para o ambiente aquático (Dourado et al, 2016).…”

Treated wastewater impact on rural green farm life cycle, Egypt

“…In this study cytotoxicity for water quality testing decides if STWW is safe for farm irrigation. Factors as root growth and shape, occurrences of mitosis and irregular cell division can be used to assessment TREATED WASTEWATER IMPACT ON RURAL GREEN FARM LIFE CYCLE, EGYPT the mutagenicity, genotoxicity and cytotoxicity of ecological contaminant [35] . Cytotoxicity of any compound could be determined by its capability to hinder or improve cell proliferation.…”