Proximate analysis, mineral composition, phytochemical contents, antioxidant and antimicrobial activities and GC-MS investigation of various solvent extracts of cactus cladode

Abstract

Cladodes Powder Extracts (CPE) are known for their medicinal properties such as being an auxiliary treatment for obesity and gastrointestinal or cardiovascular disorders as well as lowering cholesterol levels. The presented results proved that CPE was found to be a rich source of carbohydrate, ash, moisture and minerals namely Magnesium, Calcium and Zinc which highlighted its good nutritional value. Moreover, the solvent extracts displayed appreciable level of polyphenols, flavonoids and tannins contents compounds which may explain their higher and varied antioxidant and antimicrobial properties. The time-kill assay revealed that the bactericidal concentration required for cladode to kill M. luteus should be less than 3.12 mg/mL (2MIC). Interestingly, GC-MS analyses of ethyl acetate CPE demonstrate the presence of six compounds. The overall data emphasize the nutritional potential of CP as a promising natural preservative and a substitute to the synthetic counterparts and therefore to be consumed in a healthy diet.

Evaluation of Chemical Composition, Antibacterial, Antifungal, and Cytotoxic Activity of Laurus nobilis L Grown in Saudi Arabia

“…Therefore, the extraction yields increased with increasing polarity of the solvents, indicating that both, the extracting efficiency and extractable bioactive compounds favoured solvents with high polarity. Our findings are in agreement with previous reports which show that the extraction yields increased with increasing polarity of solvents 18,19 . It is known that plants possess a wide variety of bioactive compounds, which exhibit different solubility properties with different solvents, hence screening with different solvent helps identify the most appropriate solvent for biological studies 19,20 .…”

“…According to Bakari et al, phenolic compounds are more soluble in polar organic solvents than in non-polar solvents. They reported acetone as an efficient solvent to extract total phenols and flavonoid compounds 19 . Similarly, methanol and ethanol were reported as better solvents in extracting various phenolic compounds 27 .…”

Phytochemicals, antioxidant and antimicrobial potentials and LC-MS analysis of hydroalcoholic extracts of leaves and flowers of Erodium glaucophyllum collected from Tunisian Sahara

“…The Kill-time assay is used to determine the bactericidal or bacteriostatic activity of antimicrobials. Time-kill curve analyses were performed using the same method as described by our laboratory (Bakari et al, 2016). Each experiment is carried out three times.…”

Antimicrobial, Proximate and Phytochemical Evaluation of Garlic (Allium sativum L) (Ex-Lugu Cultivars)

“…23 Previous studies have shown that p-cymene, a constituent of essential oils found in garlic, have antimicrobial effect on E. coli. 20 Thymol was also present at 3.801% and 9.822% in EA and ME, respectively. The antimicrobial effect of different concentrations of ME and conventional antibiotics against selected clinical isolates are shown in Figure 3.…”

“…The protein and carbohydrate contents were high which indicated that the Ex-Lugu garlic is a good source of nutrient for growth as well as for nerve tissue regulation. 20 Figures 1 and 2 show the Gas chromatograms of ME and EA, respectively. EA had 18 compounds.…”