Occurence and antimicrobial resistance of Arcobacter species in food and slaughterhouse samples

Elmali, Mehmet; Can, Hayriye Yeşim

doi:10.1590/1678-457x.19516

Cited by 25 publications

(22 citation statements)

References 25 publications

(37 reference statements)

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“…To determine minimal bactericidal concentration (MBC), 10 μl of broth from three replicate of all wells without microbial growth was combined in a single sample spotted on MHIIA (Liofilchem, Italy) and incubated as described above up to 72 h. Since no breakpoint values are available for Arcobacter spp., classification of strains as susceptible (S), resistant (R), or intermediate (I) was defined according to zone diameter and MIC interpretive standards for Staphylococcus spp. (erythromycin, penicillin, and vancomycin) and Enterobacteriaceae (ampicillin, gentamicin, cefotaxime, ciprofloxacin, tetracycline, chloramphenicol, nalidixic acid, kanamycin, and streptomycin) (CLSI, 2015), as also reported by Elmali and Can (2017).…”

Section: Methodsmentioning

confidence: 58%

“…The antimicrobial susceptibility of Arcobacter spp. isolated from various ecological niches has been investigated by several authors (Kabeya et al, 2004; Kayman et al, 2012; Ferreira et al, 2013, 2017; Scanlon et al, 2013; Collado et al, 2014; Rahimi, 2014; Yesilmen et al, 2014; Zacharow et al, 2015b; Aski et al, 2016; Van den Abeele et al, 2016; Elmali and Can, 2017; Rathlavath et al, 2017; Šilha et al, 2017; Soma et al, 2017; Vicente-Martins et al, 2018), but the lack of standardized protocols and interpretive criteria for the antimicrobial susceptibility testing (AST) of Arcobacter spp. is the major limitation for a comparable and univocal evaluation of antimicrobial resistance and susceptibility for these microorganisms (Ferreira et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

“…Both strains are resistant to all the ß-lactam antibiotics tested (ampicillin, penicillin, and cefotaxime) (Table 6). The resistance of A. buzleri isolates to β-lactams is widespread in seafood and water sources (Collado et al, 2014; Rathlavath et al, 2017; Šilha et al, 2017) as well as in other environments (Kayman et al, 2012; Ferreira et al, 2013; Rahimi, 2014; Yesilmen et al, 2014; Zacharow et al, 2015b; Aski et al, 2016; Van den Abeele et al, 2016; Elmali and Can, 2017; Vicente-Martins et al, 2018). Rathlavath et al (2017) found that on 40 A. butzleri isolated from shellfish, 100% resulted resistant to cefotaxime and 70% to ampicillin, while among 81 A. butzleri isolated from fish, 98.7% was resistant to cefotaxime and 72.8% to ampicillin.…”

Section: Resultsmentioning

confidence: 99%

“…High percentage of resistance toward the nalidixic acid was reported for 77.5 and 83.4% of A. butzleri isolated from shellfish by Rathlavath et al (2017) and Collado et al (2014), respectively, for 71.6% of A. butzleri isolates from fish (Rathlavath et al, 2017) and for 57.6 and 88.9% of A. butzleri isolates from water sources in two different studies (Rathlavath et al, 2017; Šilha et al, 2017). One hundred percent of A. butzleri isolated from other various sources, namely, retail food products, porcine, slaughterhouse, and dairy plant samples, by different authors, were resistant to nalidixic acid (Scanlon et al, 2013; Elmali and Can, 2017; Ferreira et al, 2017; Vicente-Martins et al, 2018). By contrast, high percentage of susceptibility toward nalidixic acid, ranging from 50 to 77.8%, was reported by Yesilmen et al (2014) and Kayman et al (2012) for A. butzleri isolated from milk and dairy products and human gastroenteritis stool samples, respectively.…”

Section: Resultsmentioning

confidence: 99%

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Genomic Characterization of Arcobacter butzleri Isolated From Shellfish: Novel Insight Into Antibiotic Resistance and Virulence Determinants

et al. 2019

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Arcobacter (A.) butzleri is an emerging pathogenic microorganism, whose taxonomy has been recently suggested to be emended to the Aliarcobacter (Al.) butzleri comb. nov. Despite extensive taxonomic analysis, only few fragmented studies have investigated the occurrence and the prevalence of virulence and antibiotic resistance determinants of this species in strains isolated from shellfish. Herein we report for the first time the whole genome sequencing and genomic characterization of two A. butzleri strains isolated from shellfish, with particular reference to the antibiotic, heavy metals and virulence determinants. This study supported the taxonomic assignment of these strains to the Al. butzleri species, and allowed us to identify antibiotic and metal resistance along with virulence determinants, also additional to those previously reported for the only two A. butzleri strains from different environments genomically characterized. Moreover, both strains showed resistance to β-lactams, vanocomycin, tetracycline and erythromycin and susceptibility to aminoglycosides and ciprofloxacin. Beside enlarging the availability of genomic data to perform comparative studies aimed at correlating phenotypic differences associated with ecological niche and geographic distribution with the genetic diversity of A. butzleri spp., this study reports the endowment of antibiotic and heavy metal resistance and virulence determinants of these shellfish-isolated strains. This leads to hypothesize a relatively high virulence of A. butzleri isolated from shellfish and prompt the need for a wider genomic analysis and for in vitro and in vivo studies of more strains isolated from this and other ecological niches, to unravel the mechanism of pathogenicity of this species, and the potential risk associated to their consumption.

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Section: Methodsmentioning

confidence: 58%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Genomic Characterization of Arcobacter butzleri Isolated From Shellfish: Novel Insight Into Antibiotic Resistance and Virulence Determinants

et al. 2019

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“…Since no interpretive criteria for antibiotic resistance profiles of Arcobacter sp. were available, the results were compared with Enterobactericeae standards of CLSI () (Elmali and Can ). Isolates showing resistance to three or more than three antibiotics of different classes were considered as MDR arcobacters (Magiorakos et al ).…”

Section: Methodsmentioning

confidence: 99%

Occurrence, virulence gene and antimicrobial susceptibility profiles of Arcobacter sp . isolated from catla ( Catla catla ) in India

Nelapati

Tumati

Thirtham

et al. 2020

Lett Appl Microbiol

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In the present study, a total of 100 catla (Catla catla—major South Asian carp, local name botcha) collected from local fish markets and aquaculture ponds were subjected for isolation and characterization of Arcobacter sp. In all, 21 Arcobacter sp. were isolated, of which 18 (85·7%) were Arcobacter butzleri and three (14%) were A. cryoaerophilus as identified by multiplex PCR. All 18 A. butzleri isolates were positive for mviN, ciaB and tlyA virulence genes, three of A. cryoaerophilus isolates carried mviN gene and none of the isolates were positive for cadF, irgA, cj1349, hecA and hecB genes. All isolates (n = 21) were resistant to penicillin (100%). Meanwhile, 71·43, 23·81, 23·81, 14·29 and 9·52% of the isolates showed resistance towards vancomycin, nalidixic acid, erythromycin, cefixime and kanamycin, respectively. Multidrug resistance was observed in 23·81% of the Arcobacter sp. isolates and none of the isolates were positive for any of the extended spectrum beta‐lactamases either by phenotypic or by molecular identification genes (blaOXA, blaSHV, blaTEM, blaCTX‐M1, blaCTX‐M2 and blaCTX‐M9 groups). The results emphasize the need to implement specific control procedures to reduce the use of antibiotics in aquaculture particularly the ones which are very important in human medicine. Significance and Impact of the Study Arcobacter species are emerging food‐ and water‐borne human pathogens. In this study, Arcobacter butzleri was predominant in fish compared to A. cryoaerohilus and A. skirrowii. Higher incidence of arcobacters in fish market samples suggests cross contamination and unhygienic handling of fish in markets. Virulence genes profile and antibiotics resistance of the Arcobacter sp. isolated in current study indicate pathogenic potential of Arcobacter sp. to humans. Occurrence of multidrug‐resistant Arcobacter sp. in fish is a major concern in food safety. To our knowledge, this is the first report of Arcobacter sp. from freshwater fish, catla (Catla catla) in India.

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Genotyping, antibiotic resistance and prevalence of Arcobacter species in milk and dairy products

Lameei

Rahimi

Shakerian

et al. 2022

Veterinary Medicine & Sci

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Background Arcobacter spp. has been considered an emerging foodborne pathogen and a hazard to human health. The dairy chain has been isolated from different sources; nevertheless, data on Arcobacter occurrence in raw milk and dairy products in Iran are still scant. Objective The present study investigates the prevalence, antimicrobial susceptibility and the presence of virulence genes of Arcobacters species isolated from milk and dairy products. Methods Then, a total of 350 raw milk samples and 400 dairy product samples were collected from dairy supply centers in Isfahan, Iran. Presumptive Arcobacter strains were obtained by enriching samples in Oxoid Arcobacter enrichment broth (AEB) followed by the filtration of enrichment product through 0.45‐μm pore size membrane filters laid onto non‐selective blood at 30°C under microaerophilic conditions. Molecular identification of Arcobacter cryaerophilus and A. butzleri was performed by Polymerase chain reaction (PCR) amplification of the 16S rRNA gene, followed by sequencing. The disc diffusion method was used to determine the antimicrobial susceptibility of isolates. Targeted resistance and virulence genes were detected using multiplex PCR. Results The results show a low recovery rate of Arcobacter spp. in milk. Arcobacters were found in all types of milk, except raw camel milk, but were absent from all dairy products. Arcobacter butzleri was the predominant species in raw milk. Detection of virulence genes shows that all virulence genes targeted were found among A. butzleri, and six (cadF, cj1349, irgA, mviN, pldA, tlyA) were found among A. cryaerophilus. All A. butzleri strains and some A. cryaerophilus strains isolated from milk were resistant to amoxicillin‐clavulanic acid and tetracycline. All A. cryaerophilus isolates from milk were susceptible to gentamycin, streptomycin, erythromycin and ciprofloxacin. The distribution of resistance genes in Arcobacter strains in milk shows that all isolates carried tet(O) and blaOXA‐61 genes. Conclusions In conclusion, the results indicate a low recovery rate of Arcobacter spp. in milk and milk products. However, a significant number of Arcobacter strains with putative virulence genes may be potential pathogens for humans and an overall increase in Arcobacter resistance to first‐line antibiotics. These results highlight the need for regular surveillance of Arcobacter strains in milk and milk products in Iran.

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Occurence and antimicrobial resistance of Arcobacter species in food and slaughterhouse samples

Cited by 25 publications

References 25 publications

Genomic Characterization of Arcobacter butzleri Isolated From Shellfish: Novel Insight Into Antibiotic Resistance and Virulence Determinants

Genomic Characterization of Arcobacter butzleri Isolated From Shellfish: Novel Insight Into Antibiotic Resistance and Virulence Determinants

Occurrence, virulence gene and antimicrobial susceptibility profiles of Arcobacter sp . isolated from catla ( Catla catla ) in India

Genotyping, antibiotic resistance and prevalence of Arcobacter species in milk and dairy products

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