Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in vivo assessment of immunomodulatory effect in mice

Abstract

Beta-glucan (BG) is a conserved cell wall components of bacteria, fungi, and yeast. BG is an immunomodulator and stimulates the host immune system. This study was performed to screen Saccharomyces cerevisiae strain with high BG, extraction of BG using different chemical extraction methods, composition analysis of BG, and evaluation of the immunomodulatory effect of high-quality BG using mice model. Ten yeast strains were screened for high BG content using total glucan extraction kit and were subjected to FT-IR analysis. The kit based extraction revealed that HII31 showed a high content of total glucan and BG. HII31 cells were subjected to four different acid/base extractions, which indicated that combination of a strong base (NaDH) and weak acid (CH 3 CDDH) extraction recovered high BG and a high ratio of polysaccharide, protein, and lipid. Further, the immunomodulatory effect of the selected BG was evaluated using mice, which suggested that low dose of HII31-BG induces the expression of selected pro-inflammatory (IL-17, IFN-γ) and anti-inflammatory cytokine (IL-10) significantly, whereas relatively high dose was required to alter the IL-6 and TGF-β expression. Dverall, the present study revealed that BG extracted from HII31 cells alters the expression of studied cytokines, which can be used as a potent immunomodulator in pharmaceutical products.Keywords: beta-glucan; Saccharomyces cerevisiae; immunomodulation; FTIR; cytokines.Practical Application: Identification of optimum extraction procedure for β-glucan (BG) from S. cerevisiae HII31 and revealed that BG can be used as a potent immune modulator in pharmaceutical products.

Valorization of Deproteinated Potato Juice Water into β-Glucan Preparation of C. utilis Origin: Comparative Study of Preparations Obtained by Two Isolation Methods

“…The spectra were also related to spectrum registered for β(1,3)/(1,6)-glucan standard of Sacharomyces cerevisiae origin purchased from Sigma company. All similarities and differences were further related to spectral data provided in the literature [2,24,34,42,45,49,50]. Figure 1 presents three spectra i.e.…”

“…Yeast species and culture parameters have considerable impact on the efficiency of yeast β-glucan purification due to differences in cell wall structure and consequently its susceptibility to disruption [22,[43][44][45]. There is no data available in respect of effectiveness of those procedures in relation to yeast other than brewer's and baker's species.…”

The Utilization of Vegetable and Fruit Wastes for Saccharomyces cerevisieae Cell Wall Based β-Glucan Production with Antioxidant Activity

“…Components that play an important role in the formation of ß-glucan are also glucose. Therefore, the highest ß-glucan mass is produced by using banana waste fermentation medium because the highest glucose content is found in banana waste so that it can maximize the ß-glucan production process (Pengkumsri et al, 2017;Pyar and Peh, 2018). The metabolism of the formation of ß-glucan is in the presence of glucose which is converted to glucose-6-phosphate wherein the presence of the enzyme phosphoglucomutase is obtained by glucose-1-phosphate and is broken down into UDP-Glucose which is the constituent component of the cell wall of S. cerevisiae (Orlean, 2012).…”

“…One source of ß-glucan is the cell wall of microorganism. One of microorganisms that have the potential strains producing ß glucan is Saccharomyces cerevisiae which is non -pathogenic and non -toxic, therefore these microorganisms are often used for fermentation of food products (Pengkumsri et al, 2017). ß-glucan and S. cerevisiae are categorized as Generally Recognized As Safe (GRAS) by the FDA (Food and Drug Administration), meaning that it has no toxicity or side effects (Leentjens et al, 2014).…”

Evaluation of Bioactivities of Morinda Tinctoria Leaves Extract for Pharmacological Applications.

“…A single-beam FTIR spectrometer (FT-IR 8400S, Shimadzu) was used to analyze the extract by following the standard KBr method [12]. An average of 32 scans was figured with a spectral range of 400-4000 cm…”