Rice genotypes for drought tolerance: morphological and transcriptional evaluation of auxin-related genes

Madabula, Frederico Pedro; Santos, Railson Schreinert dos; Machado, Nata Dienes; Pegoraro, Camila; Krüger, Mariana Madruga; Maia, Luciano Carlos da; Sousa, Rogério Oliveira de; Oliveira, Antônio Costa de

doi:10.1590/1678-4499.562

Cited by 8 publications

(7 citation statements)

References 31 publications

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“…On another note, Mashkab proved to be tolerant in terms of leaf drying under the stress conditions, as it had only a 6.2% leaf drying percentage (Figure 1A). In general, the plants challenged with water deficit conditions showed reduced shoot growth and the number of roots [59]. Our results for the shoot reduction percentage suggested that the Yasmin cultivar was least affected whereas Furat the most, among the tested cultivars towards drought stress (Figure 2A).…”

Section: Discussionmentioning

confidence: 65%

Evaluation of Iraqi Rice Cultivars for Their Tolerance to Drought Stress

et al. 2020

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Drought stress is a serious problem around the globe and particularly in the Republic of Iraq. Rice is the third most consumed crop for the Iraqi people; however, its cultivation and production is very low due to several challenges including drought. The current study was performed to evaluate five Iraqi rice cultivars along with relevant (drought-tolerant and drought-susceptible) controls under drought stress, either by treatment with 10% PEG (polyethylene glycol) or through water withholding to induce natural drought stress. The phenotypes of all the cultivars were evaluated and the transcriptional responses of key drought-responsive candidate genes, identified through the EST-SSR marker-based approach, were studied. We also studied transcript accumulation of drought-related transcriptional factors, such as OsGRASS23, OsbZIP12, and OsDREB2A. Moreover, the reference cultivars also included a drought-tolerant inter-specific cultivar Nerica 7 (a cross between Oryza sativa ssp. indica X O.glaberrima). Among the cultivars, the more drought-tolerant phenotypic characteristics and higher transcript accumulation of drought-related marker genes OsE647 and OsE1899 and transcriptional factors OsGRASS23, OsbZIP12, and OsDREB2A were observed in four (out of five) significantly drought-tolerant Iraqi cultivars; Mashkab, followed by Furat, Yasmen, and Amber 33. On another note, Amber Barka was found to be significantly drought susceptible. Mashkab and Amber Barka were found to be the most drought-tolerant and-susceptible cultivars, respectively. The identified tolerant cultivars may potentially serve as a genetic source for the incorporation of drought-tolerant phenotypes in rice.

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Section: Discussionmentioning

confidence: 65%

Evaluation of Iraqi Rice Cultivars for Their Tolerance to Drought Stress

et al. 2020

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“…The primers (Table 2) were synthesized for the auxin responsive gene OsIAA1 41 , cytokinin responsive gene RR2 42 , gibberellic acid responsive gene PBZ1 43 , ethylene responsive gene ERF063 44 and ABA responsive gene OsRab16 45 with the Primer quest tool, IDT (https://eu.idtdna.com/Primerquest/Home/Index).…”

Section: Methodsmentioning

confidence: 99%

A comprehensive study of the hormetic influence of biosynthesized AgNPs on regenerating rice calli of indica cv. IR64

Manickavasagam

Pavan

Vasudevan

2019

Sci Rep

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Rice is one of the most widely cultivated crops worldwide; however, it is not amenable to genetic manipulations, owing to its poor response to tissue culture and regeneration in vitro . To improve its response to tissue culture, we evaluated the influence of biosynthesized silver nanoparticles on callus induction, regeneration and rhizogenesis in Indica rice cv. IR64. Silver nanoparticles were biosynthesized by using silver nitrate and Parthenium hysterophorus plant extract, and were characterized by UV-visible spectroscopy, Fourier-transform infrared spectroscopy, Transmission electron microscopy and X-ray diffraction. The biosynthesized silver nanoparticles (PHAgNPs), when supplemented in tissue culture medium, promoted callus induction frequency, callus regeneration and rhizogenesis at concentrations of 10 mg l −1 , 5 mg l −1 and 10 mg l −1 , respectively. Further examination of the endogenous hormonal levels in regenerating calli revealed that AgNPs enhanced regeneration by alleviating abscisic acid and ethylene levels in the plant tissue. The stimulatory influence eliciting the regeneration response was found to be optimal with the supplementation of 5 mg l −1 PHAgNPs in the regeneration medium; the malondialdehyde, proline and hydrogen peroxide levels were also lower than those in the control, thus suggesting improved antioxidant status. Our results indicated that biosynthesized PHAgNPs may have the potential to positively influence tissue culture of recalcitrant varieties.

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“…Over expression of OsDREB1 or Arabidopsis DREB also improved drought and chilling tolerance in rice (Ito et al, 2006). Madabula et al, (2016) were evaluated 4 genes related to auxin response and root modifications (OsGNOM1/ CRL4, OsIAA1, OsCAND1 and OsRAA1). The expression of these genes were analyzed in stressed rice using public available microarray data and then through real-time quantitative polymerase chain reaction (RT-qPCR), in the 6 phenotypically evaluated Brazilian genotypes under standard conditions (absence of stress).Result showed that all genotypes lengthened its roots in response to drought, specially the 2 hybrids.…”

Section: Transgenicsmentioning

confidence: 99%