Associative bacterial diversity of pangolão, a stressresilient tropical grass

Abstract

Some forage species, such as pangolão grass (Digitaria eriantha Steud. cv. Survenola), are resilient in tropical semiarid regions. A possible reason for this is the presence of endophytic and rhizospheric microorganisms. Thus, this study evaluated the diversity of associative bacteria in pangolão grass. Bacteria associated with the roots, culm, leaves, and rhizospheric soil were isolated and characterized in three municipalities of Pernambuco, Brazil. An initial phenotypic characterization was followed by a genotypic assessment by based repetitive extragenic palindromic-polymerase chain reaction (BOX-PCR) and partial sequencing of the 16S rRNA gene. We obtained 325 phenotypically-characterized isolates grouped into 243 strains with 100% similarity by BOX-PCR. The most diverse sampling environment was Araripina, and all factors affected bacterial diversity. There were 135 groups with 90% similarity, that were represented by a single strain each for sequencing. Among the sequenced strains, 118 showed 96.84-99.9% similarity with previously described strains, whereas 17 could not be identified. The following 18 genera were identified from three phyla, five classes, seven orders, and 13 families: Achromobacter,