Sugarcane pre-sprouted seedlings produced with beneficial bacteria and arbuscular mycorrhizal fungi

Abstract

The demand for the increased productivity of sugarcane crops has required changes in its production chain, such as the use of pre-sprouted seedlings (PSS) of sugarcane into the chain. In this system, the inoculation of beneficial microorganisms, such as plant growthpromoting bacteria (PGPB) and arbuscular mycorrhizal fungi (AMF), can improve seedling development. The objective was to evaluate the beneficial effect of PGPB and AMF inoculation on sugarcane PSS production system. Experiments were carried out in greenhouse using a commercial substrate with different levels of fertilization to evaluate the plant biomass and nutritional status. The bacteria strains are able to produce indole acetic acid and to amplify the nifH gene. The coinoculation of strains IAC-BeCa-095 with AMF (Glomus macrocarpum and Glomus etunicatum) improved the plant shoot biomass (25%) on the fertilized substrate. The strains IAC-BeCa-088 (Burkholderia caribensis), IAC-RBca5 (Pseudomonas sp.) and IAC-RBca10 (Bacillus sp.) without AMF and fertilization improved the shoot biomass by up to 35%. Coinoculation with strain IAC-BeCa-095 (Kosakonia radicincitans) and AMF improved the shoot and root biomass by up to 27 and 75%, respectively, in the conventionally fertilized substrate, demonstrating a synergistic effect of these microorganism consortia. The use of beneficial microorganisms may be a viable practice in the production of PSS sugarcane. Moreover, this study is the first to demonstrate the synergistic effect of endophytic bacteria (K. radicincitans) or rhizobacteria (Bacillus sp.) with AMF and Pseudomonas sp. or B. caribensis, without AMF inoculum on the production of sugarcane PSS to improve plant growth and plant nutrition.

Plant Growth-Promoting Bacteria Enhance Survival, Growth, and Nutritional Content of Sugarcane Propagated through Pre-Sprouted Seedlings under Water Deficit

“…In the absence of inoculation, PSS survival of 82.4% corroborates with the results found by Almeida Neto et al [67]. Although no other studies evaluate PSS survival in the field under the use of microorganisms, studies reported the PGPB action in improving rooting and nutritional aspects [37,[68][69][70][71].…”

Asterarcys Quadricellulare Triggers Bioactive Amines Promoting Sugarcane Sprout and Growth

“…Apical propagules have a higher sprouting rate than basal propagules due to their physiological age and greater metabolic activity (Baracat et al 2017;Figueiredo et al 2020). The vegetal growth in sugarcane can be improved with the application of organic fertilizers (Gazola et al 2017); isolated plant growth-promoting bacteria (PGPB) (Almeida et al 2024), combined with humic acids (Oliveira et al 2018), in association with arbuscular mycorrhizal fungi (Rossetto et al 2021); or through microalga biomass (Mógor et al 2022). Microalgae brings advantages to the economy and environment sustainability, producing a variety of agricultural products (Kapoore et al 2021;Parmar & Srivatsan 2023).…”