Rhizobium rhizogenes-mediated transformation of Rhodiola rosea leaf explants

Martinez, Mariana Vaquero; Barba‐Espín, Gregorio; Favero, Bruno Trevenzoli; Lütken, Henrik

doi:10.1590/1678-4499.20190428

Cited by 3 publications

(2 citation statements)

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“…First, the R. rhizogenes transformation, an important bio‐sustainable alternative to chemical growth retardants, has been widely used for the generation of compact ornamental plants since rol genes trigger plant dwarfism with varying degrees (Christensen et al 2008, Zhu et al 2015, Hegelund et al 2018). Secondly, the interest has also been focused on plant metabolic engineering since R. rhizogenes and single rol genes are powerful inducers of bioactive molecules, such as secondary metabolites and recombinant proteins (Bulgakov 2008, Shkryl et al 2008, Bulgakov et al 2013, Martínez et al 2020). However, increased attention has been recently paid to the potential use of R. rhizogenes strains or single rol genes to confer stress resilience to plants, e.g., drought resistance (Arshad et al 2014, Bulgakov et al 2018, Veremeichik et al 2022).…”

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confidence: 99%

“…Secondly, the interest has also been focused on plant metabolic engineering since R. rhizogenes and single rol genes are powerful inducers of bioactive molecules, such as secondary metabolites and recombinant proteins (Bulgakov 2008, Bulgakov et al 2013, Martínez et al 2020. However, increased attention has been recently paid to the potential use of R. rhizogenes strains or single rol genes to confer stress resilience to plants, e.g., drought resistance (Arshad et al 2014, Bulgakov et al 2018).…”

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confidence: 99%

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Rhizobium rhizogenes rolC gene promotes leaf senescence and enhances osmotic stress resistance in Arabidopsis: the positive role of abscisic acid

Chen,

Favero,

Nardy

et al. 2024

Physiologia Plantarum

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The root oncogenic loci (rol) C oncogene has been identified as a key player during the plant‐Rhizobium rhizogenes interaction and is considered to confer resistance to plant abiotic stresses, yet the underlying mechanisms remain largely elusive. In this study, Arabidopsis plants constitutively overexpressing rolC were produced. Biometric analysis showed that rolC induced diverse phenotypic modifications, including dwarfism, increased number of stem branches (1.3 times more than WT), weak root growth, early flowering (1.6 days earlier than WT) and premature leaf senescence. In addition, senescence stimulus (exogenous ethylene: 10 or 100 μL L−1) and 10% (w/v) polyethylene glycol 6000 (PEG) treatments demonstrated that rolC mediated leaf abscisic acid (ABA) enhancement in Arabidopsis, and this might be involved in rolC‐induced premature leaf senescence and resistance enhancement to osmotic stress. It is concluded that rolC‐induced premature leaf senescence may be involved in an abiotic stress escape mechanism in Arabidopsis, which is closely related to the increase of endogenous ABA levels. These findings provide new insights into the role of rolC in plant‐bacterial interaction and uncover the potential of biotechnological application associated with R. rhizogenes/rol genes in plant drought defense.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Rhizobium rhizogenes rolC gene promotes leaf senescence and enhances osmotic stress resistance in Arabidopsis: the positive role of abscisic acid

Chen,

Favero,

Nardy

et al. 2024

Physiologia Plantarum

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In vitro DIRECT SHOOT REGENERATION FROM Rhodiola rosea L. LEAF EXPLANTS

Matvieieva

2023

Biotechnol. acta

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Wild plant species are of great interest as a source of pharmacologically valuable compounds but a great number of them are endemic and/or endangered ones. Modern plant biotechnology can provide reliable methods for their utilization without disturbing natural populations. In vitro culture methods for Rhodiola species are being intensively developed to include them into various biotechnological programmes. Aim. Development of a protocol for direct Rhodiola rosea L. plant regeneration from leaf explants. Methods. The leaves of R. rosea aseptically growing plants were used as the explants. Several variants of Murashige and Skoog (1962) agar-solidified culture medium supplemented with different combinations of auxins (1-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D)) and cytokinins (kinetin and 6-benzylaminopurine (BAP)) were estimated as potential regeneration-inducing media. Regeneration frequency was calculated as the percentage of leaves that produced shoots. Results. The use of MS medium supplemented with 2.5 mg/l BAP and 1.0 mg/l 2,4-D allowed inducing shoot formation with 100% frequency. An increase in the 2,4-D content up to 2.5 mg/l and decrease in BAP content to 1.0 mg/l resulted in decreasing of the regeneration frequency to 62.5%. Regeneration frequency was 25% and 62%, respectively, on the media containing 1.0 mg/l kinetin + 2.5 mg/l 2,4-D and 2.5 mg/l kinetin + 1.0 mg/l 2,4-D. Conclusions. R. rosea leaf explants have demonstrated high regeneration capacity with using the studied combinations of plant growth regulators. MS medium supplemented with 2.5 mg/l BAP and 1.0 mg/l 2,4-D allowed inducing shoot regeneration in leaf explants with the frequency of 100%. The frequency of regeneration was lower in the case of substitution of BAP for kinetin. The other types of morphogenesis (formation of adventitious roots and/or callus) were also observed.

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