Cytomolecular characterization of cultivars and landraces of wheat tolerant and sensitive to aluminum toxicity

Oliveira, Érica Cristina de; Pinto-Maglio, Cecília Alzira Ferreira

doi:10.1590/1678-4499.2016.278

Cited by 3 publications

(5 citation statements)

References 37 publications

(37 reference statements)

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“…Wheat (Triticum aestivum) is one of the most important crops in the maintaining of the security of the food supply and is the second most consumed cereal worldwide (Galetto et al, 2017;Franco et al, 2018;Oliveira and Pinto-Maglio, 2017). Drought is one of the main constraints affecting wheat production, and is found in virtually all climatic regions, providing a huge challenge to local farming in many countries worldwide (Lobell et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Association mapping of drought tolerance indices in wheat: QTL-rich regions on chromosome 4A

Ballesta

Mora

Pozo

2020

Sci. agric. (Piracicaba, Braz.)

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Drought is likely the main abiotic stress that affects wheat yield. The identification of drought-tolerant genotypes represents an effective way of dealing with the continuous decrease in water resources as well as the increase in world population. The aim of this study was to identify single nucleotide polymorphisms (SNP) associated with drought tolerance indices in wheat by using a genome-wide association study (GWAS) under fully irrigated and rain-fed conditions. The drought tolerance indices (i.e., Stress Susceptibility Index, Stress Tolerance Index, Tolerance Index and Yield Stability Index) were calculated based on grain yield, 1,000-kernel weight and kernels per spike. The association panel was genotyped using genotyping-bysequencing (GBS). A total of 175 SNPs exhibited statistical evidence of association with at least one drought tolerance index, explaining up to 6 % of the phenotypic variation. Forty-five SNPs were associated with more than one tolerance index (up to 4 agronomic traits). Most associations were located on chromosome 4A, supporting the hypothesis that this chromosome has a key role in drought tolerance which should be exploited for wheat improvement. In addition, statistical analysis detected SNPs associated with tolerance indices in both growing seasons, providing information about genetic regions with stable effects under different environmental conditions. This GWAS experiment serves as one of the few studies on association mapping for drought tolerance indices in wheat, which could increase the efficiency of rain-fed and irrigated crop production.

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Section: Introductionmentioning

confidence: 99%

Association mapping of drought tolerance indices in wheat: QTL-rich regions on chromosome 4A

Ballesta

Mora

Pozo

2020

Sci. agric. (Piracicaba, Braz.)

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“…Mitotic analysis of durum wheat genotypes (Beni suef-1, Beni suef-3 and Sohag-1) allowed to observe complements with 2n = 4x = 28 chromosomes and with karyotype formula of 26m + 2sm. The bread wheat cultivar (Sids-8, Sids-12, Sids-13 and Giza-171) presented 2n = 6x = 42 chromosomes and karyotype formula of 34m + 8sm (de Oliveira and Pinto-Maglio, 2017). Chromosomal complements with 2n = 28 and 2n = 42 chromosomes have been recorded already in durum wheat and common wheat separately by different writers, for example, Abd Abd El-Twab (2006), Schubert, (2007, Endo et al (2014), and Pang, et al, (2014).…”

Section: Discussionmentioning

confidence: 99%

Cytomolecular Genetic Diversity Assessments of Two Wheat Species Grows in Egypt.

Bekhit

Salim

2019

Journal of Agricultural Chemistry and Biotechnology

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Genetic diversity among three genotypes of tetraploid wheat and four of hexaploid wheat, it was estimated using inter simple sequence repeats (ISSR) and cytogenetic studies. Three genotypes of Triticum dicoccum (Sohag-1, Beni-suef-1 and Beni-suef-3 belonging to Tetraploid wheat and four hexaploid genotypes; Sids-8, Sids-12, Sids-13 and Giza-171 (Triticum aestivum)) sampled from upper and lower Egypt regions; the three durum wheat cultivars and the four hexaploid wheat were assessed. Genetic diversity within wheat genotypes was evaluated using 10 ISSR primers. Of the approximately 431 detected ISSR markers, 117 (27%) were polymorphic with 27 bands per utilized primer pair. Cluster analysis of seven genotypes belonging to the two species by UPGMA cluster analysis based on Jaccard's similarity estimates for ISSR data separated all genotypes into two major clusters depend nearly on their genome makeup. The first one include wheat species possesses AB genomes, while second cluster included wheat genotypes ABD genomes. The genetic similarity coefficients ranged from 0.05 between Beni-suef 3 (Triticum dicoccum) and Sids-8 and Sids-12 of T. aestivum. Concerning chromosome morphology analysis of the three durum wheat genotypes of, the highest value for chromosome length was observed in Sohag-1 (14.84 μm for chromosome 2 A) and the smaller value in Beni-suef 3(5.16 μm for the chromosome 7B). The CI values (centromere index) obtained for durum wheat ranged from 0.48 for 5B to 0.97 for 7A. Thus, according to the CI values for karyotypes of Sohag 1, all chromosomes are metacentric except 5B chromosome. The CI values (centromere index) obtained for durum wheat ranged from 0.50 for 5B to 1.00 for 7A

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“…Wicker and Keller (2007) reported that the number of copies of transposable elements was related to the size of the genome of its hosts. Thus, this fact is backed up by the study of karyotyping of five wheat genotypes, which included BH 1146, IAC 1003, Langdon, P19, and P33 (Oliveira and Pinto-Maglio, 2017), where one of the parameters estimated in the characterization of the genotypes was the calculation of the total length of the chromatin (CTC) of the metaphase chromosomes, and was precisely where higher values of CTC were found for the P19 and P3 genotypes (302.30 ± 1.46 and 319.62 ± 1.69, respectively) compared to the CTC of the durum wheat cv IAC 1003 and Langdon (246.30 ± 1.10 and 244.40 ± 1.41, respectively).…”

Section: Fluorescence In Situ Hybridization With the Probe Sequence Omentioning

confidence: 99%

“…This common pattern of signals exhibited both repeatability and stability ( Figure 3). Chromosomes were identified based on the karyotype available in Oliveira and Pinto-Maglio (2017). From this observation, we may conclude that these two signals, related to the ALMT1 sequence and located on the short arm of the chromosomes mentioned above, were transferred from the cv BH 1146 to the P19 and P33 genotypes and that they are in fact linked to resistance to aluminum toxicity since they were identified in the three genotypes resistant to aluminum toxicity (BH 1146, P19, and P33) but were absent from the Anahuac and IAC 1003 cultivars (sensitive to Al 3+ ).…”

Section: Fluorescence In Situ Hybridization With the Probe Sequence Omentioning

confidence: 99%

“…From this knowledge of chromosome characterization (Oliveira and Pinto-Maglio, 2017) and FISH mapping of the ALMT1 gene, introduction of only the chromosome, or chromosome segment carrier of the factor that confers resistance to aluminum toxicity to durum wheat cultivars sensitive to Al 3+ , becomes a possiblity. This transfer can be accomplished through introgression lines, without changing the other positive agronomic traits of the durum wheat cultivars that are sensitive to Al 3+ , which results in savings on the number of backcrosses necessary after hybridization.…”

Section: Fluorescence In Situ Hybridization With the Probe Sequence Omentioning

confidence: 99%

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Cytogenetic mapping of the ALMT (aluminum-activated malate transporter) gene in wheat genotypes

Oliveira

Pinto-Maglio

2020

Sci. agric. (Piracicaba, Braz.)

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Presence of the ALMT1 (aluminum-activated malate transporter) gene confers resistance to aluminum toxicity in Triticum aestivum (common wheat). No resistant cultivars of Triticum turgidum ssp. Durum Desf. (durum wheat) have been registered in Brazil. The aim of this study was to map the ALMT1 through application of the FISH (fluorescence in situ hybridization) technique in five wheat genotypes, common and durum, from the Active Germplasm Bank (AGB) of the Instituto Agronômico (IAC): BH 1146, P19, P33, Anahuac, and IAC 1003. FISH-ALMT1 signals were registered in Anahuac (sensitive) chromosomes and in BH 1146, P19, and P33 (resistant) chromosomes. In the three resistant genotypes, a characteristic double FISH signal was found, located in different chromosomes of the complements: in BH 1146 in chromosome 5D, in P19 in 3B, and in P33 in 6B. This FISH-ALMT1 mapping allows for introgression of aluminum resistance in sensitive cultivars through breeding programs using introgression lines containing these carrier chromosomes.

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Cytomolecular characterization of cultivars and landraces of wheat tolerant and sensitive to aluminum toxicity

Cited by 3 publications

References 37 publications

Association mapping of drought tolerance indices in wheat: QTL-rich regions on chromosome 4A

Association mapping of drought tolerance indices in wheat: QTL-rich regions on chromosome 4A

Cytomolecular Genetic Diversity Assessments of Two Wheat Species Grows in Egypt.

Cytogenetic mapping of the ALMT (aluminum-activated malate transporter) gene in wheat genotypes

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