Use of microsatellites for evaluation of genetic diversity in cherry tomato

Ceballos-Aguirre, Nelson; López, Walter Ricardo; Orozco-Cárdenas, Martha L.; Coronado, Ana Cruz Morillo; Cabrera, Franco Alirio Vallejo

doi:10.1590/1678-4499.116

Cited by 9 publications

(5 citation statements)

References 23 publications

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“…The PIC values ranged from 0.0 to 0.68 and the mean value was 0.44. Microsatellite markers used to study genetic variability in tomato populations found a mean PIC of 0.63, with a maximum value of 0.81 and a minimum of 0.17 (Aguirre et al, 2017). Moreover, the lower mean value reported in the present study is due to the greater amount of polymorphic markers used in comparison to the others studies, which explains the mean value of lower PIC.…”

Section: Resultscontrasting

confidence: 65%

Genetic Dissimilarity in Conyza sumatrensis Revealed by Simple Sequence Repeat (SSR) Markers

et al. 2020

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In view of the rapid evolution of Conyza sumatrensis populations resistant to glyphosate, it is necessary to understand the genetic diversity aimed to improve strategies for managing this weed. We investigated the genetic dissimilarity among 15 biotypes of C. sumatrensis from different geographic regions using microsatellite loci. The biotypes, were cultivated in a greenhouse to obtain vegetal material for DNA extraction. Nineteen microsatellite markers (SSR), were developed for C. sumatrensis biotypes. The genetic dissimilarity was estimated by the Jaccard coefficient (JC) and the biotypes grouped by the UPGMA method. The results demonstrated a high dissimilarity (JC = 7.14 to 82.62) of the analyzed material, with the biotypes forming five groups, being one group formed just by the susceptible biotype and in the others grouped by biotypes from distinct locations in the same group The high genetic diversity of C. sumatrensis indicates that the biotypes may show different responses to different management strategies, and that the mechanisms of resistance to herbicides and characteristics of evolution of populations due to adaptability may be some of the factors involved in the genetic variability of the species.

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Section: Resultscontrasting

confidence: 65%

Genetic Dissimilarity in Conyza sumatrensis Revealed by Simple Sequence Repeat (SSR) Markers

et al. 2020

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“…Thus, the markers developed within this study and their relationship to the various parameters considered provide opportunities to select parents for crossbreeding in tomato breeding programmes, which is an important measure to generate new Bolivian cultivars [ 42 ]. The present study also contributed novel knowledge on the genetic diversity and population structure of Bolivian tomatoes, as previous studies have only included Bolivian accessions of cherry tomatoes [ 43 ] and its wild relatives S. lycopersicum var. ceraciforme and S. neorickii [ 9 ].…”

Section: Discussionmentioning

confidence: 92%

Simple Sequence Repeat Markers Reveal Genetic Diversity and Population Structure of Bolivian Wild and Cultivated Tomatoes (Solanum lycopersicum L.)

Gutierrez

Johansson

Prieto–Linde

et al. 2022

Genes

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The western part of South America is a centre of diversity for tomatoes, but genetic diversity studies are lacking for parts of that region, including Bolivia. We used 11 simple sequence repeat (SSR) markers (including seven novel markers) to evaluate genetic diversity and population structure of 28 accessions (four modern cultivars, four advanced lines, nine landraces, 11 wild populations), and to compare their genetic variation against phenotypic traits, geographical origin and altitude. In total, 33 alleles were detected across all loci, with 2–5 alleles per locus. The top three informative SSRs were SLM6-11, LE20592 and TomSatX11-1, with polymorphism information content (PIC) of 0.65, 0.55 and 0.49, respectively. The genetic diversity of Bolivian tomatoes was low, as shown by mean expected heterozygosity (He) of 0.07. Analysis of molecular variance (AMOVA) revealed that 77.3% of the total variation was due to variation between accessions. Significant genetic differentiation was found for geographical origin, cultivation status, fruit shape, fruit size and growth type, each explaining 16–23% of the total variation. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) tree and principal coordinate analysis (PCoA) scatter plot both revealed differentiation between accessions with determinate flowers and accessions with indeterminate flowers, regardless of cultivation status. The genetic profiles of the accessions suggest that the Bolivian tomato gene pool comprises both strictly self-pollinating and open-pollinating genotypes.

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“…With all SSR markers was obtained a lower number of detectable bands, sometimes weak, or no bands for the last three plants (number 8, 9 and 10) acclimatized to ex vitro conditions (Figure 4). SSRs have been the most widely used markers for genotyping plants over the past 25 years because they are highly informative, codominant, multi-allele genetic markers that are experimentally reproducible and transferable among related species (Gheorghe et al, 2010;Shirasawa et al, 2014;Aguirre et al, 2017;Al-Shammari and Hamdi 2021). However, when using this type of molecular marker to distinguish between various genotypes, we must consider that SSRs have a high mutation rate.…”

Section: Morphological Aspects Comparisons Between Regenerated and Do...mentioning

confidence: 99%

Androgenic ability and plant regeneration potential in some tomato varieties

Bădulescu

Dumitru

Manolescu

et al. 2022

Not Bot Horti Agrobo

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Aiming to evaluate the in vitro regeneration potential, five varieties of tomatoes (Solanum lycopersicum) were studied for their response in anther culture. Anther explants at an early stage of microspore development were inoculated onto three culture media. The first differentiation processes were recorded during the first three weeks of culture, in darkness. The statistical analysis of the data recorded during the anther culture showed significant differences between genotypes regarding their specific response to culture conditions and the significant influence of the initiation medium composition in triggering the differentiation processes. Under the tested conditions were induced: the embryogenic potential in three genotypes (ʻȘtefănești 22ʼ, ʻCostate 21ʼ and ʻChihlimbarʼ) and the indirect organogenesis in two genotypes (ʻArgeș 20ʼ and ʻCostate 21ʼ). Morphological characteristics of anther-derived plants from ʻArgeș 20ʼ variety, grown in greenhouse conditions (growth rate, features of leaf, flower, and fruit), as well as analyses with nine SSR markers (banding patterns, the coefficient of genetic similarity, and the polymorphism information content) in DNA samples from each regenerant and the donor variety, provided clear evidence of the occurrence of spontaneous genetic variation during in vitro anther culture, and of the existence of somaclonal variation in regenerated plants. The amplified products obtained with SSR primers revealed a total number of scorable bands of 160 and a mean percentage of polymorphic bands of 21.09%. Two out of the nine tested primers, SSR63 and SLM6-7 proved to be efficient in detecting genetic differences not only among regenerants but also between them and the donor genotype.

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Use of microsatellites for evaluation of genetic diversity in cherry tomato

Cited by 9 publications

References 23 publications

Genetic Dissimilarity in Conyza sumatrensis Revealed by Simple Sequence Repeat (SSR) Markers

Genetic Dissimilarity in Conyza sumatrensis Revealed by Simple Sequence Repeat (SSR) Markers

Simple Sequence Repeat Markers Reveal Genetic Diversity and Population Structure of Bolivian Wild and Cultivated Tomatoes (Solanum lycopersicum L.)

Androgenic ability and plant regeneration potential in some tomato varieties

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