Guinea Pigs Naturally Infected by Leishmania enriettii: Clinical Analyses, Parasite Isolation and Identification

Soccol, Vanete Thomaz; Castro, Edilene Alcântara de; Reifur, Larissa; Teixeira, Valéria Natascha; Lange, Rogério Ribas; Luz, Edna Dora Martins Newman

doi:10.1590/1678-4324-75years-2021210095

Cited by 2 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the previous papers, the ability of L. enriettii to infect several organs, including spleen, liver, adrenal glands, bone marrow, lymphatic ganglia and even blood, was reported. (14,18,19,23) Here, we reported for the first time the infection in trachea and lungs. By the time abovementioned papers were published, molecular tools were not available, and the techniques were mainly restricted to parasitological and microscopical methods.…”

Section: Discussionmentioning

confidence: 75%

“…(20,21) However, this is different from previous studies with infected field-collected guinea pigs, where the severity of the lesions seemed to have an impact in those animals. (14,22) We suggest that in nature a higher severity of the lesions may provide more infective blood meal to the vector. However, a proven vector for L. enriettii is yet to be determined.…”

Section: Discussionmentioning

confidence: 93%

“…After 12 weeks postinfection (PI) regardless the use of SGEs, the CL lesions healed spontaneously. Early and recent reports (14,18,21,22,23) already demonstrated the ability of L. enriettii to visceralise in guinea pigs after several inoculation routes. It was, however, unknown if this phenomenon is permanent or transient requiring fast transmission to vector(s).…”

mentioning

confidence: 99%

“…It did not detect the parasite in the wild reservoirs reinforcing the role of C. porcellus as a the main, if not the only, domestic host. (14) Several research groups have been studied L. enriettii to understand its biology and some aspects of hostparasite interaction. (15,16) A distinguished feature of this species (unlike other Mundinia members) is its ability to specifically infect guinea pigs.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Leishmania enriettii visceralises in the trachea, lungs, and spleen of Cavia porcellus

Alves-Sobrinho

Pinheiro

Paranaíba

et al. 2022

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

BACKGROUND Leishmania (Mundinia) enriettii is a species commonly found in the guinea pig, Cavia porcellus. Although it is a dermotropic species, there is still an uncertainty regarding its ability to visceralise during Leishmania life cycle.OBJECTIVE Here, we investigated the ability of L. enriettii (strain L88) to visceralise in lungs, trachea, spleen, and liver of C. porcellus, its natural vertebrate host.METHODS Animals were infected sub-cutaneously in the nose and followed for 12 weeks using histological (hematoxilin-eosin) and molecular tools (polymerase chain reaction-restriction fragment length polymorphism -PCR-RFLP). To isolate parasite from C. porcellus, animals were experimentally infected for viscera removal and PCR typing targeting hsp70 gene.FINDINGS Histological analysis revealed intense and diffuse inflammation with the presence of amastigotes in the trachea, lung, and spleen up to 12 weeks post-infection (PI). Molecular analysis of paraffin-embedded tissues detected parasite DNA in the trachea and spleen between the 4th and 8th weeks PI. At the 12th PI, no parasite DNA was detected in any of the organs. To confirm that the spleen could serve as a temporary site for L. enriettii, we performed additional in vivo experiments. During 6th week PI, the parasite was isolated from the spleen confirming previous histopathological and PCR observations. MAIN CONCLUSION Leishmania enriettii (strain L88) was able to visceralise in the trachea, lung, and spleen of C. porcellus.

show abstract

Section: Discussionmentioning

confidence: 75%