GC-MS Analysis and Bioactivity of Streptomyces sp. nkm1 Volatile Metabolites against some Phytopathogenic Fungi

Sholkamy, Essam Nageh; Palsamy, Sasikumar; Raja, S.; Alarjani, Khaloud Mohammed; Alharbi, Reem Mohammed; Abdel-Raouf, Neveen; Alsamhary, Khawla; Duraipandiyan, V.; Fredimoses, Mangaladoss; Apu, Ehsanul Hoque; Habila, Mohamed A.

doi:10.1590/1678-4324-2023220626

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…However, VOCs from B. subtilis RWBG1 contained an antifungal compound, decane, 2,3,5,8-tetramethyl-(C 14 H 30 ; MW 198.3880; syn: 2,3,5,8-Tetramethyl-decane) in addition to the above compounds. Sholkamy et al [94] reported that volatile metabolites from Streptomyces sp. nkm1 inhibited the growth of plant pathogenic fungi, including A. flavus, and the bioactive fraction contained decane, 2,3,5,8-tetramethyl-as one of its components.…”

Section: Discussionmentioning

confidence: 99%

Rice Weevil (Sitophilus oryzae L.) Gut Bacteria Inhibit Growth of Aspergillus flavus and Degrade Aflatoxin B1

Al-Saadi,

Al-Sadi,

Al-Wahaibi

et al. 2024

JoF

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In this study, bacteria residing in the gut of the rice weevils (Sitophilus oryzae L.) (Coleoptera: Curculionidae) feeding on aflatoxin-contaminated corn kernels were isolated and evaluated for their ability to suppress Aspergillus flavus and to remove/degrade aflatoxin B1 (AFB1). Four morphologically distinct S. oryzae gut-associated bacterial isolates were isolated and identified as Bacillus subtilis (RWGB1), Bacillus oceanisediminis (RWGB2), Bacillus firmus (RWGB3), and Pseudomonas aeruginosa (RWGB4) based on 16S rRNA gene sequence analysis. These bacterial isolates inhibited A. flavus growth in the dual culture assay and induced morphological deformities in the fungal hyphae, as confirmed by scanning electron microscopy. All four bacterial isolates were capable of removing AFB1 from the nutrient broth medium. In addition, culture supernatants of these bacterial isolates degraded AFB1, and the degradation of toxin molecules was confirmed by liquid chromatography-mass spectrometry. The bacterial isolates, B. subtilis RWGB1, B. oceanisediminis RWGB2, and P. aeruginosa RWGB4, were capable of producing antifungal volatile organic compounds that inhibited A. flavus growth. These results suggest that the bacterial isolates from S. oryzae gut have the potential to bind and/or degrade AFB1. Further research on their application in the food and feed industries could enhance the safety of food and feed production.

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Section: Discussionmentioning

confidence: 99%

Rice Weevil (Sitophilus oryzae L.) Gut Bacteria Inhibit Growth of Aspergillus flavus and Degrade Aflatoxin B1

Al-Saadi,

Al-Sadi,

Al-Wahaibi

et al. 2024

JoF

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“…Among the promising sources of new antibiotics, the greatest importance is in natural microbial products, especially actinomycetes, as they have a long history in the production of bioactive metabolites [2,40,45]. Since the beginning of antibiotic production in 1940, antibiotics have played an important role in antagonizing human pathogens, producing more than 50% of known antibiotics, mainly by species of the genus Streptomyces [3,41,43,44].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Actinomycetes Isolated from Soils in the Brazilian Amazon

Oliveira,

Diniz,

Peters

et al. 2024

Braz. arch. biol. technol.

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The objective of this work was to evaluate the antimicrobial activity of actinomycetes isolated from soils in the Brazilian Amazon. Soil samples were collected for the isolation of actinomycetes. The actinomycetes were grown in submerged culture and the metabolites extracted in ethyl acetate. The well diffusion method was used to verify the antimicrobial activity of isolated actinomycetes against test microorganisms. The microdilution method was used to determine the minimum inhibitory concentration (MIC) of the extracts against the test microorganisms. Bioautography was used to verify the bioactive compound of the extracts. The biomass and active metabolites were determined. 219 actinomycetes were isolated from Amazonian soils, organized into 153 morphospecies. The most frequent genus were Streptomyces (48.85%) and Nocardia (20.09%). Of the 153 extracts analyzed, 12 (7.84%) had antimicrobial activity, 9 against Gram positive bacteria and 5 against Candida albicans. The MICs of the extracts with activity ranged from 15 to 250 ug/mL. The bioautography method showed that the extracts from Streptomyces 3323 HEX, Streptomyces 3098 DCM, Streptomyces 3333 DCM and Streptomyces 3333 EtOAc generally had an active substance, and that they are different from commercial antimicrobials produced by HIGHLIGHTS• The genus Streptomyces is the most frequent actinomycete in samples of only Amazonia.• The bioautography method reveal that actinomycete extracts possess an active substance against human pathogens.• Active metabolites of actinomycetes are correlated to the stationary phase of biomass production.• Actinomycetes from the Amazon show potential to produce new antibiotics.

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GC-MS Analysis and Bioactivity of Streptomyces sp. nkm1 Volatile Metabolites against some Phytopathogenic Fungi

Cited by 2 publications

References 37 publications

Rice Weevil (Sitophilus oryzae L.) Gut Bacteria Inhibit Growth of Aspergillus flavus and Degrade Aflatoxin B1

Rice Weevil (Sitophilus oryzae L.) Gut Bacteria Inhibit Growth of Aspergillus flavus and Degrade Aflatoxin B1

Antimicrobial Activity of Actinomycetes Isolated from Soils in the Brazilian Amazon

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