GC-MS Analysis and Bioactivity of Streptomyces sp. nkm1 Volatile Metabolites against some Phytopathogenic Fungi

Abstract

The main concern of today's agricultural production around the world is a cleaner and greener approach to crop production. Streptomyces species with distinct characteristics can be found in soil. The current research focuses on Streptomyces species isolated from marine sediment samples. To evaluate the morphology and biochemical properties of the isolated Streptomyces strain ukm1, it was grown in Starch Casein Nitrate (SCN) agar medium. Morphological, biochemical, and 16s rRNA analysis were performed on the strain. Streptomyces sp. was identified based on these findings. The production of bioactive metabolites by strain nkm1 was carried out in five different fermentative media. After 10 days of incubation, M6 medium was found to be efficient and was extracted with ethyl acetate. The MIC values revealed that the ethyl acetate extract inhibited the growth of plant pathogenic fungi such as Phytophthora palmivora (31.25 g/ml), Aspergillus flavus (15.62 g/ml), Rhizoctonia solani (31.25 g/ml), and Cladosporium herbarum (31.25 g/ml). The GC-MS analysis of the ethyl acetate extract revealed 26 components, the major volatile bioactive compound being nonadecane.

Rice Weevil (Sitophilus oryzae L.) Gut Bacteria Inhibit Growth of Aspergillus flavus and Degrade Aflatoxin B1

“…However, VOCs from B. subtilis RWBG1 contained an antifungal compound, decane, 2,3,5,8-tetramethyl-(C 14 H 30 ; MW 198.3880; syn: 2,3,5,8-Tetramethyl-decane) in addition to the above compounds. Sholkamy et al [ 94 ] reported that volatile metabolites from Streptomyces sp. nkm1 inhibited the growth of plant pathogenic fungi, including A. flavus , and the bioactive fraction contained decane, 2,3,5,8-tetramethyl- as one of its components.…”

Antimicrobial Activity of Actinomycetes Isolated from Soils in the Brazilian Amazon

“…Among the promising sources of new antibiotics, the greatest importance is in natural microbial products, especially actinomycetes, as they have a long history in the production of bioactive metabolites [2,40,45]. Since the beginning of antibiotic production in 1940, antibiotics have played an important role in antagonizing human pathogens, producing more than 50% of known antibiotics, mainly by species of the genus Streptomyces [3,41,43,44].…”